Psychrophilic Lipase from Arctic Bacterium

Ramle, Zakiah; Rahim, Rashidah Abdul

doi:10.21315/tlsr2016.27.3.21

Cited by 10 publications

(6 citation statements)

References 5 publications

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“…Lipase-producing potentiality was investigated based on the size of the clear zone around each colony (Ramle and Abdul Rahim, 2016). Results indicated that all isolates were positive producers of lipase with different efficiency as the lipolytic zone diameter range was 6–20 mm.…”

Section: Resultsmentioning

confidence: 99%

Production of Cold-Active Lipase by Free and Immobilized Marine Bacillus cereus HSS: Application in Wastewater Treatment

2018

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Lipases are enzymes that have the potential to hydrolyze triacylglycerol to free fatty acids and glycerol and have various applications. The aim of the present study was to isolate and screen marine bacteria for lipase production, optimize the production, and treat wastewater. A total of 20 marine bacterial isolates were obtained from the Mediterranean Sea and were screened for lipase production. All isolates were found to have lipolytic ability. The differences between the isolates were studied using RAPD-PCR. The most promising lipase producer (isolate 3) that exhibited the highest lipolytic hydrolysis (20 mm) was identified as Bacillus cereus HSS using 16S rDNA analysis and had the accession number MF581790. Optimization of lipase production was carried out using the Plackett–Burman experimental design with cotton seed oil as the inducer under shaking conditions at 10°C. The most significant factors that affected lipase production were FeSO4, KCl, and oil concentrations. By using the optimized culture conditions, the lipase activity increased by 1.8-fold compared with basal conditions. Immobilization by adsorption of cells on sponge and recycling raised lipase activity by 2.8-fold compared with free cells. The repeated reuse of the immobilized B. cereus HSS maintained reasonable lipase activity. A trial for the economic treatment of oily wastewater was carried out. Removal efficiencies of biological oxygen demand, total suspended solids, and oil and grease were 87.63, 90, and 94.7%, respectively, which is promising for future applications.

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Section: Resultsmentioning

confidence: 99%

Production of Cold-Active Lipase by Free and Immobilized Marine Bacillus cereus HSS: Application in Wastewater Treatment

2018

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“…Psychrotolerants are organisms that can withstand a broader range of temperatures between 0 °C to approximately 36 °C [25]. Some researchers called them facultative psychrophiles or psychrotrophs and their maximal or optimal growth temperature was above 20 ºC [26,27]. Based on the OFAT, all isolated strains showed the fastest growth rate at 20 °C.…”

Section: Discussionmentioning

confidence: 99%

The Optimization of Growth Condition of the Bacteria Producing Cold-Active Proteolytic Enzyme from the Antarctic Region

Latip¹,

Nordin²,

Alias³

et al. 2023

IIUMEJ

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The growth conditions of bacteria producing cold-active protease isolated from an Antarctic sample were screened using one-factor-at-time (OFAT). Then, crude protease of the strain was extracted during the late logarithmic phase for enzymatic assay. A strain that showed the highest enzyme activity was selected for optimization via response surface method (RSM). The parameters studied were incubation temperature (4 – 36 °C), pH media (4 – 10) and NaCl concentration (0 – 8%). Based on the OFAT results, all eight strains showed the highest growth rate at 20 °C, pH 7 and 4% (w/v) NaCl. The assay showed that the crude enzyme extracted from strain SC8 exhibited significantly higher activity (0.20 U and 0.37 U) than the positive control (0.11 U and 0.31 U) at -20 °C and 20 °C. RSM suggested that the optimized setting for growth of SC8 were at 20.5 °C, pH 6.83 and 2.05% (w/v) of NaCl with the results of the bacterial growth rate value was 3.70 ± 0.06 x 106 cells/hr. Optimal growth conditions of SC8 from this study are useful for the large-scale production of cold-active protease in future. ABSTRAK: Keadaan pertumbuhan bakteria yang menghasilkan enzim protease aktif sejuk daripada sampel Antartika disaring menggunakan satu faktor pada masa (OFAT). Kemudian, enzim protease ini diekstrak pada lewat fasa logaritma untuk ujian enzimatik. Strain yang menunjukkan aktiviti enzim tertinggi telah dipilih untuk tujuan pengoptimuman melalui kaedah permukaan tindak balas (RSM). Parameter yang dikaji ialah suhu pengeraman (4 – 36 °C), pH media (4 – 10) dan kepekatan NaCl (0 – 8%). Berdasarkan OFAT, kesemua lapan bakteria menunjukkan kadar pertumbuhan tertinggi pada 20 °C, pH 7 dan 4% NaCl. Hasil ujian enzimatik menunjukkan bahawa enzim protease yang diekstrak daripada SC8 mempamerkan aktiviti yang jauh lebih tinggi (0.20 U dan 0.37 U) daripada kawalan positif (0.11 U dan 0.31 U) pada -20 °C dan 20 °C. RSM mencadangkan tetapan optimum untuk pertumbuhan SC8 adalah pada 20.5 °C, pH 6.83 dan 2.05% NaCl dengan keputusan kadar pertumbuhan bakteria ialah 3.70 ± 0.06 x 106 sel/jam. Keadaan pertumbuhan optimum SC8 daripada kajian ini bermanfaat untuk menghasilkan produk protease aktif sejuk secara besar-besaran pada masa hadapan. The growth conditions of bacteria producing cold-active protease isolated from an Antarctic sample were screened using one-factor-at-time (OFAT). Then, crude protease of the strain was extracted during the late logarithmic phase for enzymatic assay. A strain that showed the highest enzyme activity was selected for optimization via response surface method (RSM). The parameters studied were incubation temperature (4 – 36 °C), pH media (4 – 10) and NaCl concentration (0 – 8%). Based on the OFAT results, all eight strains showed the highest growth rate at 20 °C, pH 7 and 4% (w/v) NaCl. The assay showed that the crude enzyme extracted from strain SC8 exhibited significantly higher activity (0.20 U and 0.37 U) than the positive control (0.11 U and 0.31 U) at -20 °C and 20 °C. RSM suggested that the optimized setting for growth of SC8 were at 20.5 °C, pH 6.83 and 2.05% (w/v) of NaCl with the results of the bacterial growth rate value was 3.70 ± 0.06 x 106 cells/hr. Optimal growth conditions of SC8 from this study are useful for the large-scale production of cold-active protease in future. ABSTRAK: Keadaan pertumbuhan bakteria yang menghasilkan enzim protease aktif sejuk daripada sampel Antartika disaring menggunakan satu faktor pada masa (OFAT). Kemudian, enzim protease ini diekstrak pada lewat fasa logaritma untuk ujian enzimatik. Strain yang menunjukkan aktiviti enzim tertinggi telah dipilih untuk tujuan pengoptimuman melalui kaedah permukaan tindak balas (RSM). Parameter yang dikaji ialah suhu pengeraman (4 – 36 °C), pH media (4 – 10) dan kepekatan NaCl (0 – 8%). Berdasarkan OFAT, kesemua lapan bakteria menunjukkan kadar pertumbuhan tertinggi pada 20 °C, pH 7 dan 4% NaCl. Hasil ujian enzimatik menunjukkan bahawa enzim protease yang diekstrak daripada SC8 mempamerkan aktiviti yang jauh lebih tinggi (0.20 U dan 0.37 U) daripada kawalan positif (0.11 U dan 0.31 U) pada -20 °C dan 20 °C. RSM mencadangkan tetapan optimum untuk pertumbuhan SC8 adalah pada 20.5 °C, pH 6.83 dan 2.05% NaCl dengan keputusan kadar pertumbuhan bakteria ialah 3.70 ± 0.06 x 106 sel/jam. Keadaan pertumbuhan optimum SC8 daripada kajian ini bermanfaat untuk menghasilkan produk protease aktif sejuk secara besar-besaran pada masa hadapan.

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“…The bacterial isolate was obtained from an Artic soil sample, ARC4, collected by the Polar Research Group of Universiti Sains Malaysia (USM) ( 87 ). The sampling took place in August 2011, on the northern coast of Hornsund, Wedel Jarlsberg Land, West Spitsbergen ( o 00′04″N, 15°33′37″E).…”

Section: Methodsmentioning

confidence: 99%

“…The bacterial isolate was cultivated in Luria-Bertani (LB) agar plates at different temperatures: 15°C, 24°C, and 37°C for 48 h to test whether the isolate fit the definition of obligate psychrophile or facultative psychrotroph ( 87 ). Obligate psychrophiles are organisms that prefer temperatures close to 0 for growth ( 70 ).…”

Section: Methodsmentioning

confidence: 99%

Characterization of Sphingobacterium sp. Ab3 Lipase and Its Coexpression with LEA Peptides

Ikeno

Almansoori

et al. 2022

Microbiol Spectr

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Psychrophilic Lipase from Arctic Bacterium

Cited by 10 publications

References 5 publications

Production of Cold-Active Lipase by Free and Immobilized Marine Bacillus cereus HSS: Application in Wastewater Treatment

Production of Cold-Active Lipase by Free and Immobilized Marine Bacillus cereus HSS: Application in Wastewater Treatment

The Optimization of Growth Condition of the Bacteria Producing Cold-Active Proteolytic Enzyme from the Antarctic Region

Characterization of Sphingobacterium sp. Ab3 Lipase and Its Coexpression with LEA Peptides

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