Psychrophilic pseudomonas in antarctic freshwater lake at stornes peninsula, larsemann hills over east Antarctica

Chauhan, Abhishek; Bharti, Pawan Kumar; Goyal, Pankaj; Varma, Ajit; Jindal, Tanu

doi:10.1186/s40064-015-1354-3

Cited by 22 publications

(7 citation statements)

References 13 publications

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“…Studies using a culture-dependent approach showed that Pseudomonas spp. are (sub)dominant among cultivated bacteria in the Arctic and Antarctic (Chauhan et al 2015, Romaniuk et al 2018), which is in line with our data. Pseudomonads well tolerate various environmental conditions due to their high metabolic versatility (Ozen & Ussery 2012).…”

Section: Discussionsupporting

confidence: 92%

Bacteriophages and their microbial hosts in terrestrial biotopes of Antarctica

et al. 2021

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Virus diversity in Antarctic biotopes remains understudied. Here, we describe bacteriophages isolated from terrestrial environments, provide data on their natural bacterial hosts and study phage-host systems. Six bacterial isolates (FCKU 539, FCKU 533, FCKU 534, FCKU 538, FCKU 542 and FCKU 540) were recovered and characterized. Isolated bacteria belonged to Pseudomonas genus (Pseudomonas sp., Pseudomonas fluorescens, Pseudomonas putida) with optimal cultivation temperatures of 16–28°C. These bacteria and previously described Bacillus subtilis FCKU 537 were used for analysing virus-host interactions. Six lytic phages were isolated and named P. fluorescens Antarctic virus 1 (PFAV1), P. fluorescens Antarctic virus 2 (PFAV2), P. fluorescens Antarctic virus 3 (PFAV3), P. putida Antarctic virus 4 (PPAV4), Pseudomonas sp. Antarctic virus 5 (PSAV5) and B. subtilis Antarctic virus 6 (BSAV6) in relation to their natural hosts. According to electron microscopy data, these phages belonged to Caudovirales order. Cross-inoculation demonstrated high specificity of all Antarctic phages, which infected only their initial hosts at moderate temperatures. PFAV2 and PFAV3 phages also infected laboratory Pseudomonas savastanoi and P. fluorescens isolates. This paper adds new data on the occurrence and diversity of viruses and their respective bacterial hosts in soil biotopes of Antarctica.

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Section: Discussionsupporting

confidence: 92%

Bacteriophages and their microbial hosts in terrestrial biotopes of Antarctica

et al. 2021

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“…Moreover, the purchased frozen food was distributed by a single supplier for all farms. Pseudomonadaceae can survive at freezing temperature (Lu et al, 2011; Chauhan et al, 2015). Therefore, our initial hypothesis was that P. aeruginosa could be selected compared to other freezing-sensitive gut microbiota species and replicate in defrosted food.…”

Section: Discussionmentioning

confidence: 99%

The prevalence ofPseudomonas aeruginosaand multidrug resistantPseudomonas aeruginosain healthy captive ophidian

Sala

Ianni

Pelizzone

et al. 2019

PeerJ

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Background Snakes are globally considered as pet animals, and millions of ophidians are bred in captivity. Pseudomonas aeruginosa is a ubiquitous Gram-negative bacterium that can act as an opportunistic pathogen of man and animals and is frequently present in the oral and cloacal microbiota of healthy ophidians. It can cause severe clinical diseases and often shows antibiotic resistance. The aim of this study was to evaluate the prevalence and antibiotic resistance profiles of P. aeruginosa isolated from the cloacal microbiota of a large population sample of healthy captive ophidians and to evaluate the statistical associations with farming conditions. Methods A total of 419 cloacal swabs were collected from snakes belonging to the Boidae (n = 45), Colubridae (n = 48) and Pythonidae (n = 326) families and inoculated onto complete culture media. Food, water and bedding samples were also analyzed. The antimicrobial susceptibility of P. aeruginosa isolates was evaluated through the Kirby-Bauer agar diffusion test. Statistical analyses were performed with the chi-square test. Results The prevalence of P. aeruginosa was 59.9%, and 35.5% of these strains were multidrug resistant (MDR). The prevalence of MDR P. aeruginosa was significantly higher in adult samples than in young samples, and widespread resistance to Cephalosporins, Polymyxins and Sulfonamides was observed. Statistically significant differences in the prevalence of P. aeruginosa were observed depending on the farm size and snake family. Feeding thawed prey was associated with a higher P. aeruginosa and MDR P. aeruginosa prevalence. Moreover, snakes fed home-raised prey had a significantly higher MDR P. aeruginosa prevalence than snakes fed commercially available feed. Less frequent terrarium cleaning was associated with a higher MDR P. aeruginosa prevalence. On the other hand, snake reproductive status was not significantly associated with P. aeruginosa or MDR P. aeruginosa prevalence. All food, water and bedding samples were negative for P. aeruginosa presence. Discussion The overall P. aeruginosa prevalence found in this study was lower than that found by other authors, but a high proportion of the isolates were MDR. This study highlighted the presence of constitutive (such as age and taxonomic family) and managerial (farm size, cleaning cycle frequency and food type) factors associated with P. aeruginosa and/or MDR P. aeruginosa prevalence. Good breeding management and proper antibiotic treatment of P. aeruginosa infections could help reduce the presence of P. aeruginosa and MDR P. aeruginosa in the gut microbiota of snakes and consequently reduce the risk to public health.

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“…Pseudomonas spp. were also found in permanently cold, polar regions, including Arctic and Antarctic marine waters, glaciers and soil [6,7,8,9]. Studies applying a culture-dependent approach revealed that Pseudomonas spp.…”

Section: Introductionmentioning

confidence: 99%

Diversity and Horizontal Transfer of Antarctic Pseudomonas spp. Plasmids

Romaniuk

Styczyński

Decewicz

et al. 2019

Genes

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Pseudomonas spp. are widely distributed in various environments around the world. They are also common in the Antarctic regions. To date, almost 200 plasmids of Pseudomonas spp. have been sequenced, but only 12 of them were isolated from psychrotolerant strains. In this study, 15 novel plasmids of cold-active Pseudomonas spp. originating from the King George Island (Antarctica) were characterized using a combined, structural and functional approach, including thorough genomic analyses, functional analyses of selected genetic modules, and identification of active transposable elements localized within the plasmids and comparative genomics. The analyses performed in this study increased the understanding of the horizontal transfer of plasmids found within Pseudomonas populations inhabiting Antarctic soils. It was shown that the majority of the studied plasmids are narrow-host-range replicons, whose transfer across taxonomic boundaries may be limited. Moreover, structural and functional analyses enabled identification and characterization of various accessory genetic modules, including genes encoding major pilin protein (PilA), that enhance biofilm formation, as well as active transposable elements. Furthermore, comparative genomic analyses revealed that the studied plasmids of Antarctic Pseudomonas spp. are unique, as they are highly dissimilar to the other known plasmids of Pseudomonas spp.

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Psychrophilic pseudomonas in antarctic freshwater lake at stornes peninsula, larsemann hills over east Antarctica

Cited by 22 publications

References 13 publications

Bacteriophages and their microbial hosts in terrestrial biotopes of Antarctica

Bacteriophages and their microbial hosts in terrestrial biotopes of Antarctica

The prevalence ofPseudomonas aeruginosaand multidrug resistantPseudomonas aeruginosain healthy captive ophidian

Diversity and Horizontal Transfer of Antarctic Pseudomonas spp. Plasmids

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