Psychrotrophic yeast Yarrowia lipolytica NCYC 789 mediates the synthesis of antimicrobial silver nanoparticles via cell-associated melanin

Apte, Mugdha; Sambre, Devashree; Gaikawad, Shital; Joshi, Suresh G.; Bankar, Ashok; Kumar, Anuj; Zinjarde, Smita

doi:10.1186/2191-0855-3-32

Cited by 139 publications

(50 citation statements)

References 49 publications

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“…We have got very similar AFM results for the characterization of the silver and ZnO nanoparticles observed in some previous studies, respectively [30,32]. We have got similar functional groups through FTIR analysis in some previous studies for the confirmation of AGNPs and ZnONPs, respectively [25,33]. On the other hand, in a recent study, they have observed DPPH scavenging activity of synthesized silver nanoparticles extracted from leaf samples and they have found highest activity as 4.30 µg/ml [34].…”

Section: Discussionsupporting

confidence: 60%

Green Synthesis and Characterization of Marine Yeast-Mediated Silver and Zinc Oxide Nanoparticles and Assessment of Their Antioxidant Activity

Aswathy

Gabylis

et al. 2017

Asian J Pharm Clin Res

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Objective: The present study focuses on the synthesis of silver and zinc oxide (ZnO) nanoparticles from marine yeasts, isolated from the sediments of the Bay of Bengal, Bakkhali coast, West Bengal, and India. Methods:The marine sediment samples were diluted through serial diution and cultured onto yeast malt agar medium by the spread plate method. The selected yeast isolates were screened for the biosynthesis of silver and ZnO nanoparticles. Characterization of both the nanoparticles was done by applying ultraviolet (UV)-visible spectroscopy, atomic force microscopy, scanning electron microscopy, and Fourier transform infrared spectroscopy.Results: A total of five marine yeasts isolates were able to synthesize silver and ZnO nanoparticles as evidence of the color change. Optical density was measured in UV-spectrometer at different time interval for the conformation of production of nanoparticles. The size of silver nanoparticle was 31.78 nm and ZnO nanoparticle was 86.27 nm. The synthesized nanoparticles are then used for antioxidant assays. Conclusions:We are concluding that marine yeast isolates SAG1 and SAG2 both are potential marine yeast isolates which can synthesize both the silver and ZnO nanoparticles. They also showed good antioxidant activity.

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Section: Discussionsupporting

confidence: 60%

Green Synthesis and Characterization of Marine Yeast-Mediated Silver and Zinc Oxide Nanoparticles and Assessment of Their Antioxidant Activity

Aswathy

Gabylis

et al. 2017

Asian J Pharm Clin Res

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“…However, there are fewer reports on their use for mediating the AgNPs production in comparison to those reporting on the use of bacterial extracts. [36][37][38][39] Pigmented yeasts R. glutinis and R. mucilaginosa isolated in this study were observed to be able to reduce Ag + and to produce homogenous colloidal AgNPs from AgNO 3 (1.0 mmol L -1 ) when this salt is dissolved in their fungal extract. The nanoparticle formation after 168 h was confirmed by its plasmon absorption band, manifesting at a wide range of the UV-Vis absorption spectrum (see Figure S1 in the SI file).…”

Section: Characterization Of Synthetic and Biogenic Agnpsmentioning

confidence: 70%

Silver Nanocoatings at Large Length Scales: Influence of the AgNPs Morphology and Capping Agents on the Coating Chemical Stability and Antimicrobial Effect

Sousa¹,

Noronha²,

Machado³

et al. 2016

J. Braz. Chem. Soc.

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We assessed at multiple length scales (nanometers to millimeters) the nanocoatings of silver nanoparticles (AgNPs) on model SiO 2 /Si substrates. The coatings from biogenic AgNPs (from yeasts Rhodotorula glutinis and Rhodotorula mucilaginosa) were compared to those formed from "synthetic" AgNPs capped with citrate and sodium dodecyl sulfate (SDS). With computational analysis of large-field (LF) X-ray images of the whole substrates (5 × 5 mm), we were able to assess the coatings homogeneity, relative amount of AgNPs, and their distribution as agglomerates. Surprisingly, by analyzing more than 100,000 elements (nanoparticles and agglomerates) in each sample, it was observed that the mentioned features have little dependence on the AgNPs morphology and capping agents. All silver nanocoatings resisted when immersed in phosphatebuffered saline medium by forming agglomerates of up to 10 μm 2 . However, coatings formed with synthetic AgNPs (capped with citrate and SDS) led to a higher antimicrobial efficiency against Staphylococcus aureus.Keywords: nanobiotechnology, image processing, confocal laser scanning microscopy, scanning electron microscopy, large-field X-ray imaging IntroductionSilver nanoparticles (AgNPs) have many technological applications as films and coatings over solid surfaces such as metals, ceramic, glasses and polymers, to prevent growth of bacteria and fungi. [1][2][3][4][5] For the AgNPs to be successfully attached, a suitable match between the solid surface and the nanoparticle physicochemical characteristics must occur. By synthesizing AgNPs with the mediation of bacteria and fungi extracts (biogenic AgNPs or bio-AgNPs), the resulting protein-stabilizing capping agent over the nanoparticle contains a variety of chemical groups bonded to the amino acids fragments. This latter aspect may lead to a myriad of possible chemical interactions (e.g. electrostatic, hydrophobic, hydrogen bonds and van der Waals) between the nanoparticles and solid surfaces. [6][7][8] Essentially, bio-AgNPs are formed from interaction mechanisms ruled by proteins present in the bacterial/ fungal extract or filtrate, which drive the bio-AgNPs' nucleation → growth → colloidal stabilization steps. 2,9,10 At the end of the kinetic process, a protein corona phase on the bio-AgNPs provides a long-term colloidal stability. In contrast, "synthetic" AgNPs are produced from the reduction of Ag + and commonly stabilized by small capping agents of low molecular complexity, such as citrate and sodium dodecyl sulfate (SDS). 11,12 Several insightful studies in the literature [13][14][15] reported the formation of coatings and films of AgNPs (both biogenic and synthetic) and their dependence on the chemical functionalization of the solid surfaces upon which they are attached. However, the characterization of these coatings are limited to small length scales (up to microns), commonly achieved through imaging techniques such as electron microscopy performed at high magnifications.1,2,4,16-25 A complete understanding of the Silver Nanocoating...

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“…These compounds improve the potential for these strains to produce metal nanoparticles as prominent building blocks in nanotechnology (Pimprikar et al, 2009). Some potential strains of Y. lipolytica may be used to generate elemental metal nanoparticles (Agnihotri et al, 2009;Apte et al, 2013). This field of research is still in its infancy.…”

Section: Production Of Nanoproductsmentioning

confidence: 99%

Yarrowia lipolytica as an Oleaginous Cell Factory Platform for Production of Fatty Acid-Based Biofuel and Bioproducts

2014

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Today's biotechnologists seek new biocatalysts to meet the growing demand for the bioproducts. This review critically evaluates the potential use of Y. lipolytica as an oleaginous cell factory platform. This yeast has undergone extensive modifications for converting a wide range of hydrophobic and hydrophilic biomass, including alkane, oil, glycerol, and sugars to fatty acid-based products. This article highlights challenges in the development of this platform and provides an overview of strategies to enhance its potential in the sustainable production of biodiesel, functional dietary lipid compounds, and other value-added oleochemical compounds. Future applications of the recombinant Y. lipolytica platform are also discussed.

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Psychrotrophic yeast Yarrowia lipolytica NCYC 789 mediates the synthesis of antimicrobial silver nanoparticles via cell-associated melanin

Cited by 139 publications

References 49 publications

Green Synthesis and Characterization of Marine Yeast-Mediated Silver and Zinc Oxide Nanoparticles and Assessment of Their Antioxidant Activity

Green Synthesis and Characterization of Marine Yeast-Mediated Silver and Zinc Oxide Nanoparticles and Assessment of Their Antioxidant Activity

Silver Nanocoatings at Large Length Scales: Influence of the AgNPs Morphology and Capping Agents on the Coating Chemical Stability and Antimicrobial Effect

Yarrowia lipolytica as an Oleaginous Cell Factory Platform for Production of Fatty Acid-Based Biofuel and Bioproducts

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