PtAUREO1a and PtAUREO1b knockout mutants of the diatom Phaeodactylum tricornutum are blocked in photoacclimation to blue light

Mann, Marcus; Serif, Manuel; Jakob, Torsten; Kroth, Peter G.; Wilhelm, Christian

doi:10.1016/j.jplph.2017.05.020

Cited by 34 publications

(16 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another study has shown the positive impact of supplementing different light colors on Chlamydomonas reinhardtii culture [13]. Through various studies, it was suggested that different classes of photoreceptors have specific responses to specific light mediated by different light-signaling pathways that are yet to be completely elucidated [2,37,38]. The other physiological phenomenon that can change the culture conditions is the rate of photoinhibition, which seems to be higher in W as compared to R. The energy of each photon is inversely proportional to the wavelength.…”

Section: Growth Curve Analysis Of P Tricornutum During Red Light Shiftmentioning

confidence: 99%

Red Light Variation an Effective Alternative to Regulate Biomass and Lipid Profiles in Phaeodactylum tricornutum

et al. 2020

View full text Add to dashboard Cite

Marine water diatom Phaeodactylum tricornutum is a photosynthetic organism that is known to respond to the changing light environment and adapt to different temperatures to prevent photoinhibition and maintain its metabolic functions. The objective of the present study was to test whether light shift variations in different growth phases impact the growth and lipid metabolism of P. tricornutum. Thus, we investigated R exposure in different growth phases to find the most effective light shift condition. The results showed that substituting white light (W) by red light (R) under autotrophic conditions, a condition called red shift (RS), increased biomass and lipid content compared to levels found under continuous W or R exposure alone. We observed an increase by 2-fold biomass and 2.3-fold lipid content in RS as compared to W. No significant change was observed in the morphology of lipid droplets, but the fatty acid (FA) composition was altered. Specifically, polyunsaturated FAs were increased, whereas monounsaturated FAs decreased in P. tricornutum grown in RS compared to W control. Therefore, we propose that a light shift during the beginning of the stationary phase is a low-cost cultivation strategy to boost the total biomass and lipids in P. tricornutum.Appl. Sci. 2020, 10, 2531 2 of 18 for the high production of desired bioactive molecules. Therefore, light should be provided with specific spectral components, intensity, and duration. Multiple ecophysiological studies have been published reporting on the distinct behaviors of different diatoms and algae in response to variations in light [4,5]. It has been shown that light quality has an impact on chloroplast migration and on the light acclimation reactions of photosynthesis [6,7]. Red light has been linked to stimulating growth, ethylene production [8], lipid accumulation [7], and increasing thylakoid stacking [6]. have suggested the involvement of phytochromes in increasing the intracellular nitrate content on exposure to red light for short periods altering the overall cellular nitrate content. Recent discoveries in genomics have revealed exciting information on phytochromes, blue-light sensing cryptochromes, and aureochromes [10]. In a study by Jungandreas et al. [11], it has been reported that P. tricornutum cells acclimated to both red and blue light have comparable metabolite profiles, but drastic changes in metabolites and carbon partitioning was observed under red to blue light shift. In addition, various interdisciplinary studies have shown that light perceived by the red-light receptor (phytochromes) can regulate nutrient metabolism, cellular events, and signaling cascades [12]. These discoveries are still in progress and much remains unknown, but these studies can be explored for application-based diatom experiments such as developing low-cost cultivation technology or efficient bioreactors.In both culture medium and natural water bodies, light (intensity, distribution, photoperiod) may vary and will not behave similar to the dissolved salts or nu...

show abstract

Section: Growth Curve Analysis Of P Tricornutum During Red Light Shiftmentioning

confidence: 99%

Red Light Variation an Effective Alternative to Regulate Biomass and Lipid Profiles in Phaeodactylum tricornutum

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Since in AUREO1a-silenced strains remnant AUREO1a protein could be present, or might be functionally replaced by one of the other aureochromes, AUREO1a and AUREO1b knock-out mutants of P. tricornutum have been generated by genome editing via TALEN technology (Serif et al, 2017). The results of this study has been published by Mann et al (2017), showing for the first time that AUREO1a-deficient mutants are strongly impaired in acclimation to high BL, as documented by a strong reduction in both photosynthetic and NPQ capacity. In contrast, in RL the differences between the AUREO1a deficient mutant and the wild type cells is smaller, but significant.…”

Section: Phenotypes Of Aureochrome1a and Aureochrome1b Knock-out Mutamentioning

confidence: 99%

An update on aureochromes: Phylogeny – mechanism – function

Kroth

Wilhelm

Kottke

2017

Journal of Plant Physiology

Self Cite

View full text Add to dashboard Cite

Light is important for algae, as it warrants metabolic independence via photosynthesis. In addition to the absorption of light by the photosystems, algae possess a variety of specific photoreceptors that allow the quantification of the light fluxes as well as the assessment of light qualities. About a decade ago, aureochromes have been described in the xanthophyte alga Vaucheria frigida. These proteins represent a new type of blue light photoreceptor as they possess both a light-oxygen-voltage (LOV) domain for light reception as well as a basic region leucine zipper (bZIP) domain for DNA binding, indicating that they represent light-driven transcription factors. Aureochromes so far have been detected only in a single group of algae, photosynthetic stramenopiles, but not in any other prokaryotic or eukaryotic organisms. Recent biophysical work on aureochromes in the absence and the presence of DNA revealed the mechanism of allosteric communication between the sensor and effector domains despite their unusual inversed arrangement. Different molecular models have been proposed to describe the effect of light on DNA binding. Functional characterization of mutants of the diatom Phaeodactylum tricornutum, in which the aureochrome genes have been silenced or deleted, indicate that different aureochromes may have different functions, being involved in central processes like light acclimation and regulation of the cell cycle.

show abstract

“…Yeast two-hybrid screening has demonstrated a strong interaction between SjAUREO and the 40S ribosomal protein S6, which might be involved in blue light-mediated cellular division and photomorphogenesis [10]. In the single cellular diatom Phaeodactylum tricornutum, PtAUREO1a and PtAUREO1b mutants exhibit signi cantly decreased photoacclimation to blue light [11,12]. AUREO1 in Heterosigma akashiwois may act as a blue light-sensitive negative gene regulator, which is driven by dark-to-light transitional cues [13].…”

Section: Introductionmentioning

confidence: 99%

miR8181 is Involved in the Cell Growth and Development Regulation of Saccharina Japonica

Yang

Wang

Jian

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Aureochrome, a blue-light receptor found in photosynthetic stramenopiles, plays an important role in brown algal growth and development. Aureochrome preserves the reversed effector-sensor domain for blue light reception and acts as the candidate optogenetic tool for light induced post-transcriptional regulation, but the inner rapid regulation of aureochrome remains to be studied. MicroRNA (miRNAs) of plant can cleavage the specific base-pairing site of mRNA by RNA interference mechanism, and such post-transcriptional regulation of miRNAs to photoreceptor has received attention due to the flexible regulation pathway. However, the targeting relationship between aureochrome and miRNA is unclear.Results: In this study, the potential regulatory network between miRNAs and aureochrome were explored by transcriptome and sRNA sequencing in Saccharina japonica. Our results found that 18 miRNAs perfectly paired with aureochrome. Among the screened miRNAs, miR8181-x was negatively correlated with aureochrome5 with high credibility and exhibited tissue-specific expression in S. japonica. Degradome sequencing detected the exact cleavage site of miR8181-x on aureochrome5, confirming their targeting regulation relationship. Among the 54 target genes of miR8181-x, nine genes of ABC transporters, E3 ubiquitin-protein protein ligase, Hsp90, Mx1, PetC, EF2, GSA, HAD-superfamily hydrolase and SET2 that exhibited similar expression with aureochrome5 competed with the same binding site, thus constructing the competing endogenous RNA network. Functional analysis of miR8181-x target genes revealed that regulation of cell differentiation and development was enriched, indicating the potential role of miR8181-x in the regulation of growth and development.Conclusion: Our study found that miR8181-x negatively regulated the expression of aureochrome5. The exact cleavage site in aureochrome5 were verified by degradome sequencing, confirming the targeting relationships. Functional enrichment of miR8181-x target genes revealed that miR8181-x involved in the cell growth and development regulation of S. japonica.

show abstract

PtAUREO1a and PtAUREO1b knockout mutants of the diatom Phaeodactylum tricornutum are blocked in photoacclimation to blue light

Cited by 34 publications

References 27 publications

Red Light Variation an Effective Alternative to Regulate Biomass and Lipid Profiles in Phaeodactylum tricornutum

Red Light Variation an Effective Alternative to Regulate Biomass and Lipid Profiles in Phaeodactylum tricornutum

An update on aureochromes: Phylogeny – mechanism – function

miR8181 is Involved in the Cell Growth and Development Regulation of Saccharina Japonica

Contact Info

Product

Resources

About

PtAUREO1a and PtAUREO1b knockout mutants of the diatom Phaeodactylum tricornutum are blocked in photoacclimation to blue light

Cited by 34 publications

References 27 publications

Red Light Variation an Effective Alternative to Regulate Biomass and Lipid Profiles in Phaeodactylum tricornutum

Red Light Variation an Effective Alternative to Regulate Biomass and Lipid Profiles in Phaeodactylum tricornutum

An update on aureochromes: Phylogeny – mechanism – function

miR8181&nbsp;is Involved in the&nbsp;Cell Growth and Development Regulation of&nbsp;Saccharina Japonica

Contact Info

Product

Resources

About

miR8181 is Involved in the Cell Growth and Development Regulation of Saccharina Japonica