Pteryxin: A coumarin in Peucedanum japonicum Thunb leaves exerts antiobesity activity through modulation of adipogenic gene network

In vivo and in vitro anti-obesity activities of dihydropyranocoumarins derivatives from Peucedanum japonicum Thunb

“…Although several studies reported some pharmacological activities of PJT (Adams et al, 2009;Chen et al, 1996;Hisamoto et al, 2004;Lee et al, 2004;Takeuchi et al, 1991;Yang et al, 2009) and biological activities of pyranocoumarins (Call & Fischer, 1958;Nikonov et al, 1971;Smith et al, 1957;Thastrup et al, 1983), we demonstrated the anti-obesity activities of PJT and identified PTX as one of its active compound for the first time (Nugara et al, 2014;Okabe et al, 2011). Indeed, mice that were fed an HFDcontaining CC prepared from PJT-PTX (+) gained significantly lesser weight and had lower relative WAT weights than the control mice ( Fig.…”

“…The confluent preadipocytes were maintained for another 2 days. Differentiation was induced using a standard procedure for 2 days as previously described (Nugara et al, 2014). The cells were re-fed with DMEM containing 10% FBS and 10 lg/mL of human insulin for 4 or 5 days.…”

“…In our previous studies, we found that dietary PJT and its ethanol extract decreases obesity and diabetic symptoms of mice that are fed a high-fat diet (HFD) (Nukitrangsan et al, 2012;Okabe et al, 2011). Furthermore, we recently isolated an active compound of PJT with anti-obesity properties called pteryxin [PTX, (+)-cis-3 0 -ac etoxy-4 0 -angeloyoxy-3 0 ,4 0 -dihydroseselin, PubChem CID: 5281425] (Nugara, Inafuku, Takara, Iwasaki, & Oku, 2014).…”

Effect of Peucedanum japonicum Thunb on Body Composition and Biochemical Examination of Blood

“…For chlorogenic acid, its inhibitory effect on obesity 11,12) and promotion effect of adiponectin secretion 13) have been reported. In addition, an anti-obesity effect of pteryxin has been shown by a fundamental experiment 8) .…”

“…It has been reported that PJT exhibits an anti-obesity effect in mice, and its mechanism of action is believed to be associated with chlorogenic acid, which is an ingredient of PJT 6,7) . Moreover, it has recently been reported that pteryxin, which is another ingredient of PJT, inhibits synthesis of TG 8) . Furthermore, in vitro study of glucose metabolism indicated that PJT extract has alpha-glucosidase inhibitory activity 9) .…”