PtmiR169o plays a positive role in regulating drought tolerance and growth by targeting the PtNF-YA6 gene in poplar

Jiao, Zhiyin; Lian, Conglong; Han, Shuo; Huang, Mengbo; Shen, Chao; Li, Qing; Niu, Meng-Xue; Yu, Xiao; Yin, Weilun; Xia, Xinli

doi:10.1016/j.envexpbot.2021.104549

Cited by 16 publications

(14 citation statements)

References 60 publications

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“…For example, in response to drought and ABA treatment, Bna-miR169n targets BnaNF-YA9 in Brassica napus ( Wang et al, 2022 ). PtmiR169o targets the PtNF-YA6 gene to regulate drought tolerance in poplar ( Jiao et al, 2021 ). More research into miR169-targeted PAO2 -regulated drought is required.…”

Section: Discussionmentioning

confidence: 99%

Integrated analysis of small RNAs, transcriptome and degradome sequencing reveal the drought stress network in Agropyron mongolicum Keng

Fan

Sun²,

Zhuo³

et al. 2022

Front. Plant Sci.

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Agropyron mongolicum (A. mongolicum) is an excellent gramineous forage with extreme drought tolerance, which lives in arid and semiarid desert areas. However, the mechanism that underlies the response of microRNAs (miRNAs) and their targets in A. mongolicum to drought stress is not well understood. In this study, we analyzed the transcriptome, small RNAome (specifically the miRNAome) and degradome to generate a comprehensive resource that focused on identifying key regulatory miRNA-target circuits under drought stress. The most extended transcript in each collection is known as the UniGene, and a total of 41,792 UniGenes and 1,104 miRNAs were identified, and 99 differentially expressed miRNAs negatively regulated 1,474 differentially expressed target genes. Among them, eight miRNAs were unique to A. mongolicum, and there were 36 target genes. A weighted gene co-expression network analysis identified five hub genes. The miRNAs of five hub genes were screened with an integration analysis of the degradome and sRNAs, such as osa-miR444a-3p.2-MADS47, bdi-miR408-5p_1ss19TA-CCX1, tae-miR9774_L-2R-1_1ss11GT-carC, ata-miR169a-3p-PAO2, and bdi-miR528-p3_2ss15TG20CA-HOX24. The functional annotations revealed that they were involved in mediating the brassinosteroid signal pathway, transporting and exchanging sodium and potassium ions and regulating the oxidation–reduction process, hydrolase activity, plant response to water deprivation, abscisic acid (ABA) and the ABA-activated signaling pathway to regulate drought stress. Five hub genes were discovered, which could play central roles in the regulation of drought-responsive genes. These results show that the combined analysis of miRNA, the transcriptome and degradation group provides a useful platform to investigate the molecular mechanism of drought resistance in A. mongolicum and could provide new insights into the genetic engineering of Poaceae crops in the future.

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Section: Discussionmentioning

confidence: 99%

Integrated analysis of small RNAs, transcriptome and degradome sequencing reveal the drought stress network in Agropyron mongolicum Keng

Fan

Sun²,

Zhuo³

et al. 2022

Front. Plant Sci.

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“…Integrated miRNAomic and transcriptomic analysis has suggested that expression of miR472a, miR169, miR164a, and miR396a was upregulated, while expression of miR172d and miR398 was decreased, in transgenic poplar over-expressing miR156 [ 85 ]. Furthermore, miR472a, miR172d, miR169, miR164a and their target genes are involved in plant responses to drought stress; miR396a and its target gene are heat stress-responsive regulators; and miR398 with its target genes contributes to salt tolerance of woody plants [ 24 , 25 , 26 , 35 , 52 , 86 ]. Additionally, it has been revealed that the miR156/SPL module regulates salt stress tolerance by activating MdWERKY100 expression in transgenic apple plants [ 87 ].…”

Section: Discussion and Perspectivesmentioning

confidence: 99%

“…These investigations were mostly based on high-throughput sequencing and microarray, and confirmed the universality and diversity of miRNAs in the modulation of the response of forest trees to drought stress. Furthermore, a few miRNAs and their targets have been verified in transgenic Populus in response to drought stress, including miR169o and its target subunit A of Nuclear Factor Y ( PtNF - YA6 ), miR172d and its target GT-2-like 1 (GTL1) trihelix transcription factor ( PuGTL1 ), miR472a and its target F-box 1 ( FB1 ), and miR6445 and its target NAC (NAM, ATAF, and CUC) transcription factor [ 24 , 25 , 26 ]. However, detailed functional analyses and understanding of the regulatory mechanisms of miRNAs in forest trees are yet to be reached, in contrast with the annual model plant Arabidopsis and rice.…”

Section: Mirnas Involved In the Response To Abiotic Stressmentioning

confidence: 99%

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The Intersection of Non-Coding RNAs Contributes to Forest Trees’ Response to Abiotic Stress

Xiao

Chen

Yang

et al. 2022

IJMS

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Non-coding RNAs (ncRNAs) play essential roles in plants by modulating the expression of genes at the transcriptional or post-transcriptional level. In recent years, ncRNAs have been recognized as crucial regulators for growth and development in forest trees, and ncRNAs that respond to various abiotic stresses are now under intense study. In this review, we summarized recent advances in the understanding of abiotic stress-responsive microRNAs (miRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) in forest trees. Furthermore, we analyzed the intersection of miRNAs, and epigenetic modified ncRNAs of forest trees in response to abiotic stress. In particular, the abiotic stress-related lncRNA/circRNA–miRNA–mRNA regulatory network of forest trees was explored.

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“…Salt stress suppressed the expression of maize ( Zea mays ) ZmmiR169q, and up-regulated its target gene ZmNFYA8 , finally enhancing salt tolerance in maize by attenuating ROS-induced toxicity ( Xing et al., 2021 ). In contrast, tomato ( Solanum lycopersicum ) SlymiR169c and poplar ( Populus trichocarpa ) PtmiR169o enhanced the plant’s drought resistance ( Zhang et al., 2011 ; Jiao et al., 2021 ). The miR169/NFYA module in plants is not functionally conserved in response to abiotic stresses.…”

Section: Introductionmentioning

confidence: 99%

The miR169b/NFYA1 module from the halophyte Halostachys caspica endows salt and drought tolerance in Arabidopsis through multi-pathways

Zeng²,

Zhang³

et al. 2023

Front. Plant Sci.

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Salt and drought are the major abiotic stress factors plaguing plant growth, development and crop yields. Certain abiotic-stress tolerant plants have developed special mechanisms for adapting to adverse environments in the long process of evolution. Elucidating the molecular mechanisms by which they can exert resistance to abiotic stresses is beneficial for breeding new cultivars to guide agricultural production. Halostachys caspica, a perennial halophyte belonging to Halostachys in Amaranthaceae, is extremely tolerant to harsh environments, which is commonly grown in the saline-alkali arid desert area of Northwest, China. However, the molecular mechanism of stress tolerance is unclear. Nuclear Factor Y-A (NFYA) is a transcription factor that regulates the expression of downstream genes in plant response to adverse environments. It has also been reported that some members of the NFYA family are the main targets of miR169 in plants. In this study, we mainly focused on exploring the functions and preliminary mechanism of the miR169b/NFYA1 module from H. caspica to abiotic stress. The main results showed that RLM-RACE technology validated that HcNFYA1 was targeted by HcmiR169b, qRT-PCR revealed that HcmiR169b was repressed and HcNFYA1 was induced in the H. caspica branches under various abiotic stress as well ABA treatment and Arabidopsis stable transformation platform with molecular methods was applied to elucidate that the HcmiR169b/HcNFYA1 module conferred the salt and drought tolerance to plants by enhancing ABA synthesis and ABA signal transduction pathways, maintaining ROS homeostasis and the stability of cell membrane. HcNFYA1 is expected to be a candidate gene to improve plant resistance to salt and drought stresses.

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PtmiR169o plays a positive role in regulating drought tolerance and growth by targeting the PtNF-YA6 gene in poplar

Cited by 16 publications

References 60 publications

Integrated analysis of small RNAs, transcriptome and degradome sequencing reveal the drought stress network in Agropyron mongolicum Keng

Integrated analysis of small RNAs, transcriptome and degradome sequencing reveal the drought stress network in Agropyron mongolicum Keng

The Intersection of Non-Coding RNAs Contributes to Forest Trees’ Response to Abiotic Stress

The miR169b/NFYA1 module from the halophyte Halostachys caspica endows salt and drought tolerance in Arabidopsis through multi-pathways

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