PtNPs/rGO-GluOx/mPD Directionally Electroplated Dual-Mode Microelectrode Arrays for Detecting the Synergistic Relationship between the Cortex and Hippocampus of Epileptic Rats

Xie, Jingyu; Dai, Yuchuan; Yu, Xing; Wang, Yiding; Yang, Gucheng; He, Enhui; Xu, Zhaojie; Fan, Penghui; Mo, Fan; Wu, Yidi; Song, Yilin; Cai, Xinxia

doi:10.1021/acssensors.3c00140

Cited by 12 publications

(4 citation statements)

References 36 publications

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“…Investigating how glutamate concentration changes in the BLA after LPS administration and correlating these with electrophysiological signals can reveal more characteristics of depressive neural activity. The MEA used in this study can be customized according to experimental requirements, and the sites, once modified with specific nanomaterials and enzymes, can facilitate in vivo, in situ real-time detection of glutamate [ 55 ]. Therefore, the next phase of our research will focus on the synchronous detection of in vivo electrophysiological signals and neurotransmitter signals in the mPFC and BLA, aiming to provide further neurobiological insights into the connection between inflammation and depression.…”

Section: Discussionmentioning

confidence: 99%

Microelectrode Arrays for Detection of Neural Activity in Depressed Rats: Enhanced Theta Activity in the Basolateral Amygdala

Kong,

Xu,

Yang

et al. 2024

Cyborg Bionic Syst

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Depression is a common and severely debilitating neuropsychiatric disorder. Multiple studies indicate a strong correlation between the occurrence of immunological inflammation and the presence of depression. The basolateral amygdala (BLA) is crucial in the cognitive and physiological processing and control of emotion. However, due to the lack of detection tools, the neural activity of the BLA during depression is not well understood. In this study, a microelectrode array (MEA) based on the shape and anatomical location of the BLA in the brain was designed and manufactured. Rats were injected with lipopolysaccharide (LPS) for 7 consecutive days to induce depressive behavior. We used the MEA to detect neural activity in the BLA before modeling, during modeling, and after LPS administration on 7 consecutive days. The results showed that after LPS treatment, the spike firing of neurons in the BLA region of rats gradually became more intense, and the local field potential power also increased progressively. Further analysis revealed that after LPS administration, the spike firing of BLA neurons was predominantly in the theta rhythm, with obvious periodic firing characteristics appearing after the 7 d of LPS administration, and the relative power of the local field potential in the theta band also significantly increased. In summary, our results suggest that the enhanced activity of BLA neurons in the theta band is related to the depressive state of rats, providing valuable guidance for research into the neural mechanisms of depression.

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Section: Discussionmentioning

confidence: 99%

Microelectrode Arrays for Detection of Neural Activity in Depressed Rats: Enhanced Theta Activity in the Basolateral Amygdala

Kong,

Xu,

Yang

et al. 2024

Cyborg Bionic Syst

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“…The lithium-pilocarpine model, widely recognized internationally as a drug-induced rat model of TLE resembling the clinical condition is well-established ( Reddy and Kuruba, 2013 ; Xie et al, 2023 ). In this model, activation of acetylcholine muscarinic receptors induces persistent generalized clonus, leading to seizures.…”

Section: Methodsmentioning

confidence: 99%

Utilizing GO/PEDOT:PSS/PtNPs-enhanced high-stability microelectrode arrays for investigating epilepsy-induced striatal electrophysiology alterations

Han,

Wang,

Jing

et al. 2024

Front. Bioeng. Biotechnol.

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The striatum plays a crucial role in studying epilepsy, as it is involved in seizure generation and modulation of brain activity. To explore the complex interplay between the striatum and epilepsy, we engineered advanced microelectrode arrays (MEAs) specifically designed for precise monitoring of striatal electrophysiological activities in rats. These observations were made during and following seizure induction, particularly three and 7 days post-initial modeling. The modification of graphene oxide (GO)/poly (3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS)/platinu-m nanoparticles (PtNPs) demonstrated a marked reduction in impedance (10.5 ± 1.1 kΩ), and maintained exceptional stability, with impedance levels remaining consistently low (23 kΩ) even 14 days post-implantation. As seizure intensity escalated, we observed a corresponding increase in neuronal firing rates and local field potential power, with a notable shift towards higher frequency peaks and augmented inter-channel correlation. Significantly, during the grand mal seizures, theta and alpha bands became the dominant frequencies in the local field potential. Compared to the normal group, the spike firing rates on day 3 and 7 post-modeling were significantly higher, accompanied by a decreased firing interval. Power in both delta and theta bands exhibited an increasing trend, correlating with the duration of epilepsy. These findings offer valuable insights into the dynamic processes of striatal neural activity during the initial and latent phases of temporal lobe epilepsy and contribute to our understanding of the neural mechanisms underpinning epilepsy.

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“…In terms of functionality, aside from the electrophysiological detection, electrochemical detection has also been seamlessly integrated into the MEA (Figure 4D). [ 115–117 ] For example, the use of nafion and oxidase on the modified electrode surface can convert chemical signal induced by neurotransmitter into current signal recorded by MEA. Furthermore, MEAs can be employed for the electrically stimulate of hiPSC‐DNs in vitro.…”

Section: Establishment Of An In Vitro Human Brain Detection Platformmentioning

confidence: 99%

“…In addition to the neuronal electrophysiological signal detection, neurotransmitter from synapses also is conducive to analyzing the state of neurons. [ 36,117,133 ] Typically, the waveform and intensity of the current pulses generated by vesicle exocytosis can be recorded by MEA to analyze the quantity of neurotransmitters in the vesicles. [ 35 ] Therefore, the exocytosis of neurotransmitter vesicles are quantitatively analyzed and the physiological characteristics of hiPSC‐DNs are further revealed.…”

Section: Establishment Of An In Vitro Human Brain Detection Platformmentioning

confidence: 99%

Using Human‐Induced Pluripotent Stem Cell Derived Neurons on Microelectrode Arrays to Model Neurological Disease: A Review

Lv,

He,

Luo

et al. 2023

Advanced Science

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In situ physiological signals of in vitro neural disease models are essential for studying pathogenesis and drug screening. Currently, an increasing number of in vitro neural disease models are established using human‐induced pluripotent stem cell (hiPSC) derived neurons (hiPSC‐DNs) to overcome interspecific gene expression differences. Microelectrode arrays (MEAs) can be readily interfaced with two‐dimensional (2D), and more recently, three‐dimensional (3D) neural stem cell‐derived in vitro models of the human brain to monitor their physiological activity in real time. Therefore, MEAs are emerging and useful tools to model neurological disorders and disease in vitro using human iPSCs. This is enabling a real‐time window into neuronal signaling at the network scale from patient derived. This paper provides a comprehensive review of MEA's role in analyzing neural disease models established by hiPSC‐DNs. It covers the significance of MEA fabrication, surface structure and modification schemes for hiPSC‐DNs culturing and signal detection. Additionally, this review discusses advances in the development and use of MEA technology to study in vitro neural disease models, including epilepsy, autism spectrum developmental disorder (ASD), and others established using hiPSC‐DNs. The paper also highlights the application of MEAs combined with hiPSC‐DNs in detecting in vitro neurotoxic substances. Finally, the future development and outlook of multifunctional and integrated devices for in vitro medical diagnostics and treatment are discussed.

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PtNPs/rGO-GluOx/mPD Directionally Electroplated Dual-Mode Microelectrode Arrays for Detecting the Synergistic Relationship between the Cortex and Hippocampus of Epileptic Rats

Cited by 12 publications

References 36 publications

Microelectrode Arrays for Detection of Neural Activity in Depressed Rats: Enhanced Theta Activity in the Basolateral Amygdala

Microelectrode Arrays for Detection of Neural Activity in Depressed Rats: Enhanced Theta Activity in the Basolateral Amygdala

Utilizing GO/PEDOT:PSS/PtNPs-enhanced high-stability microelectrode arrays for investigating epilepsy-induced striatal electrophysiology alterations

Using Human‐Induced Pluripotent Stem Cell Derived Neurons on Microelectrode Arrays to Model Neurological Disease: A Review

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