PtomtAPX is an autonomous lignification peroxidase during the earliest stage of secondary wall formation in Populus tomentosa Carr

Zhang, Jiaxue; Liu, Yadi; Li, Conghui; Yin, Bin; Liu, Xiatong; Guo, Xiao‐Na; Zhang, Chong; Liu, Di; Hwang, Inhwan; Li, Hui; Lu, Hai

doi:10.1038/s41477-022-01181-3

Cited by 23 publications

(18 citation statements)

References 37 publications

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“…For TUNEL results, the nucellus tissue of HG but not EG was covered with dotted PCD signals, corresponding to the nuclear position shown by 4′,6‐diamidino‐2‐phenylindole (DAPI; Figure 7i,j). Spot‐like PCD signals were also detected only in the seed coat of HG, but some lumpy fluorescence signals (white arrow) were detected in both HG and EG, which may be derived from cellular oxidation and lignin polymerization rather than CrWRKY74 (Zhang et al., 2022). These results revealed that the occurrence of the PCD process during HG and EG seed development was strictly regulated by CrWRKY74 .…”

Section: Resultsmentioning

confidence: 99%

Laser capture microdissection‐based spatiotemporal transcriptomes uncover regulatory networks during seed abortion in seedless Ponkan (Citrus reticulata)

Zhang

et al. 2023

The Plant Journal

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Seed abortion is an important process in the formation of seedless characteristics in citrus fruits. However, the molecular regulatory mechanism underlying citrus seed abortion is poorly understood. Laser capture microdissection-based RNA-seq combined with Pacbio-seq was used to profile seed development in the Ponkan cultivars 'Huagan No. 4' (seedless Ponkan) (Citrus reticulata) and 'E'gan No. 1' (seeded Ponkan) (C. reticulata) in two types of seed tissue across three developmental stages. Through comparative transcriptome and dynamic phytohormone analyses, plant hormone signal, cell division and nutrient metabolismrelated processes were revealed to play critical roles in the seed abortion of 'Huagan No. 4'. Moreover, several genes may play indispensable roles in seed abortion of 'Huagan No. 4', such as CrWRKY74, CrWRKY48 and CrMYB3R4. Overexpression of CrWRKY74 in Arabidopsis resulted in severe seed abortion. By analyzing the downstream regulatory network, we further determined that CrWRKY74 participated in seed abortion regulation by inducing abnormal programmed cell death. Of particular importance is that a preliminary model was proposed to depict the regulatory networks underlying seed abortion in citrus. The results of this study provide novel insights into the molecular mechanism across citrus seed development, and reveal the master role of CrWRKY74 in seed abortion of 'Huagan No. 4'.

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Section: Resultsmentioning

confidence: 99%

Laser capture microdissection‐based spatiotemporal transcriptomes uncover regulatory networks during seed abortion in seedless Ponkan (Citrus reticulata)

Zhang

et al. 2023

The Plant Journal

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“…When OsGLPs perform the function of SOD, they cause an increase in the H 2 O 2 content in the cell wall. The polymerisation of monolignols is the final step in lignin synthesis in the cell wall ( Perkins et al., 2022 ; Zhang et al., 2022 ). We speculated that OsGLPs could affect lignin biosynthesis through the generated H 2 O 2 .…”

Section: Discussionmentioning

confidence: 99%

“…The polymerisation of monolignols is the final step in lignin synthesis in the cell wall (Perkins et al, 2022;Zhang et al, 2022). We speculated that OsGLPs could affect lignin biosynthesis through the generated H 2 O 2 .…”

Section: Discussionmentioning

confidence: 99%

OsGLP participates in the regulation of lignin synthesis and deposition in rice against copper and cadmium toxicity

et al. 2023

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Copper (Cu) and cadmium (Cd) are common heavy metal pollutants. When Cd and excessive Cu accumulate in plants, plant growth is reduced. Our previous study showed that Germin-like proteins (GLPs), which exist in tandem on chromosomes, are a class of soluble glycoproteins that respond to Cu stress. In this study, hydroponic cultures were carried out to investigate the effect of GLP on Cd and Cu tolerance and accumulation in rice. The results showed that knockout of a single OsGLP8-2 gene or ten OsGLP genes (OsGLP8-2 to OsGLP8-11) resulted in a similar sensitivity to Cd and Cu toxicity. When subjected to Cu and Cd stress, the glp8-2 and glp8-(2-11) mutants displayed a more sensitive phenotype based on the plant height, root length, and dry biomass of the rice seedlings. Correspondingly, Cu and Cd concentrations in the glp8-2 and glp8-(2-11) mutants were significantly higher than those in the wild-type (WT) and OsGLP8-2-overexpressing line. However, Cu and Cd accumulation in the cell wall was the opposite. Furthermore, we determined lignin accumulation. The overexpressing-OsGLP8-2 line had a higher lignin accumulation in the shoot and root cell walls than those of the WT, glp8-2, and glp8-(2-11). The expression of lignin synthesis genes in the OsGLP8-2-overexpressing line was significantly higher than that in the WT, glp8-2, and glp8-(2-11). The SOD activity of OsGLP8-2, Diaminobe-nzidine (DAB), propidium iodide (PI) staining, and Malondialdehyde (MDA) content determination suggested that OsGLP8-2 is involved in heavy metal-induced antioxidant defense in rice. Our findings clearly suggest that OsGLPs participate in responses to heavy metal stress by lignin deposition and antioxidant defense capacity in rice, and OsGLP8-2 may play a major role in the tandem repeat gene clusters of chromosome 8 under heavy metal stress conditions.

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“…This finding implied the existence of a shunt in the lignin pathway to bypass multiple enzymes to form caffeic acid. A recent report on P. tomentosa mitochonrial APX (PtomtAPX) showed that the mitochondrial APX could relocate to the cell wall to assist lignin polymerization in secondary cell wall formation and xylem development [ 18 ]. As SbAPX, PtomtAPX catalyzes the dimerization of monolignols such as p -coumaryl alcohol, coniferyl alcohol and sinapyl alcohol, utilizing H 2 O 2 as a substrate [ 11 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…A recent report on P. tomentosa mitochonrial APX (PtomtAPX) showed that the mitochondrial APX could relocate to the cell wall to assist lignin polymerization in secondary cell wall formation and xylem development [ 18 ]. As SbAPX, PtomtAPX catalyzes the dimerization of monolignols such as p -coumaryl alcohol, coniferyl alcohol and sinapyl alcohol, utilizing H 2 O 2 as a substrate [ 11 , 18 ]. Thus, PtomtAPX displays a dual functionality, linking the scavenging of the ROS with lignification of the cell wall.…”

Section: Introductionmentioning

confidence: 99%

Activity of Cytosolic Ascorbate Peroxidase (APX) from Panicum virgatum against Ascorbate and Phenylpropanoids

Zhang

Lewis²,

Kovacs³

et al. 2023

IJMS

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APX is a key antioxidant enzyme in higher plants, scavenging H2O2 with ascorbate in several cellular compartments. Here, we report the crystal structures of cytosolic ascorbate peroxidase from switchgrass (Panicum virgatum L., Pvi), a strategic feedstock plant with several end uses. The overall structure of PviAPX was similar to the structures of other APX family members, with a bound ascorbate molecule at the ɣ-heme edge pocket as in other APXs. Our results indicated that the H2O2-dependent oxidation of ascorbate displayed positive cooperativity. Significantly, our study suggested that PviAPX can oxidize a broad range of phenylpropanoids with δ-meso site in a rather similar efficiency, which reflects its role in the fortification of cell walls in response to insect feeding. Based on detailed structural and kinetic analyses and molecular docking, as well as that of closely related APX enzymes, the critical residues in each substrate-binding site of PviAPX are proposed. Taken together, these observations shed new light on the function and catalysis of PviAPX, and potentially benefit efforts improve plant health and biomass quality in bioenergy and forage crops.

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PtomtAPX is an autonomous lignification peroxidase during the earliest stage of secondary wall formation in Populus tomentosa Carr

Cited by 23 publications

References 37 publications

Laser capture microdissection‐based spatiotemporal transcriptomes uncover regulatory networks during seed abortion in seedless Ponkan (Citrus reticulata)

Laser capture microdissection‐based spatiotemporal transcriptomes uncover regulatory networks during seed abortion in seedless Ponkan (Citrus reticulata)

OsGLP participates in the regulation of lignin synthesis and deposition in rice against copper and cadmium toxicity

Activity of Cytosolic Ascorbate Peroxidase (APX) from Panicum virgatum against Ascorbate and Phenylpropanoids

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