PTR-TOF-MS and HPLC analysis in the characterization of saffron (Crocus sativus L.) from Italy and Iran

Determination of Crocin Content and Volatile Components in Different Qualities of Iranian Saffron

“…The profile of the colour components of the samples was determined with HPLC by partial modification of the technique performed by Masi et al (2016) and Caballero-Ortega et al (2007). According to this techniques, 0.050 ± 0.001 g ground saffron sample was weighed, and then 10 mL methanol: water mixture (50: 50) was added and the solution was kept on a magnetic mixer at 4 °C in the dark for 24 h. After that, the acquired extract was transferred into a 15 mL centrifugal tube and centrifuged at 20,000 g at 4 °C for 20 min.…”

Integrated analytical methodology to investigate bioactive compounds in Crocus sativus L. flowers

“…Quality and authenticity studies based on the metabolic profiling of saffron have been well documented in the literature by using different analytical approaches. High‐performance liquid chromatography with diode array detector (HPLC‐DAD) was used to investigate isomers of both crocins and crocetin, safranal, picrocrocin, 4‐hydroxy‐2,6,6‐trimethyl‐1‐carboxaldehyde‐1‐cyclohexene (HTCC), and kaempferol content as well as to study the effect of drying methods on the metabolic content . Raman and FT‐IR were used to identify and quantify crocetin, dimethylcrocetin and crocins while gas chromatography with mass spectrometry (GC‐MS) was used to analyse aroma active compounds of saffron, such as isophorone, safranal, 4‐ketoisophorone, phenylethyl alcohol, 2‐pentanol, α , β ‐ionone, isophorone oxide .…”

Polyphenols and aromatic volatile compounds in biodynamic and conventional ‘Golden Delicious’ apples (Malus domestica Bork.)

“…Table 3 shows all masses highlighted by PTR-ToF-MS analysis and their possible identification, taking into account the available fragmentation patterns of pure standards (see Masi et al [26] for references). Most of the masses listed have already been identified in apples using PTR-MS approach (see references listed in Table 3).…”