Puberty onset curve in CD (Sprague Dawley) and Long Evans outbred male rats

Fuochi, Sara; Galasso, Marica E; Colombo, Rita; Giaquinto, Daniela; Girolamo, Paolo de; D’Angelo, Livia

doi:10.1177/00236772221078725

Cited by 9 publications

(4 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, the ovariectomy and castration surgery were performed at 8 weeks of age, at which time rats had reached puberty and had been exposed to sex hormones [38][39][40]. It is unknown whether the initial exposure to sex hormones modifies the regulation of monocarboxylate transporters, and further if these alterations are reversable after removing the endogenous sex hormones.…”

Section: Discussionmentioning

confidence: 99%

Effect of Sex and Cross-Sex Hormone Treatment on Renal Monocarboxylate-Transporter Expression in Rats

Wei,

Lee,

Zhang

et al. 2023

Pharmaceutics

View full text Add to dashboard Cite

Proton- and sodium-dependent monocarboxylate transporters (MCTs/SMCTs) are determinants of renal clearance through the renal reabsorption of monocarboxylate substrates. Prior studies with intact females and males, ovariectomized females and castrated males have revealed the hormonal regulation of renal monocarboxylate-transporter expression, prompting investigation into the regulatory role of individual hormones. The aim of the present study is to evaluate the effect of exogenous sex and cross-sex hormones on renal MCT1, MCT4, CD147 and SMCT1 mRNA and membrane-bound protein expression. Ovariectomized (OVX) females and castrated (CST) male Sprague Dawley rats received estrogen and/or progesterone, testosterone, or a corresponding placebo treatment for 21 days prior to kidney collection. The quantitative measurement of mRNA and membrane-protein levels were conducted using qPCR and Western blot. Quantitative analysis revealed the combination estrogen/progesterone treatment reduced membrane MCT1 and 4 expression and increased SMCT1 expression, while testosterone administration increased MCT1 membrane-protein expression. Correlation analysis indicated that plasma 17β-estradiol was negatively correlated with MCT1 and MCT4 membrane expression, while testosterone was positively correlated. In contrast, SMCT1 membrane expression was positively correlated with 17β-estradiol and progesterone concentrations. MCT1, MCT4, CD147 and SMCT1 renal expression are significantly altered in response to female and male sex hormones following sex and cross-sex hormone treatment in OVX and CST rats. Further studies are needed to understand the complex role of sex hormones, sex hormone receptors and the impact of puberty on MCT/SMCT regulation.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of Sex and Cross-Sex Hormone Treatment on Renal Monocarboxylate-Transporter Expression in Rats

Wei,

Lee,

Zhang

et al. 2023

Pharmaceutics

View full text Add to dashboard Cite

show abstract

“…Male and female rats underwent a behavioral test battery for naturalistic rewards during peripuberty (PND 28–38) or adolescence (PND 44–54). In this study, peripuberty refers to the period of time from PND 28–42, which is thought to cover the equivalent of “childhood” (P28–P30) and around puberty onset (P34–P42) in the rat (Marquez et al., 2013; Tzanoulinou, Garcia‐Mompo et al., 2014; Tzanoulinou, Riccio et al., 2014), with most female Sprague‐Dawley rats exhibiting puberty (as indexed by vaginal opening) around PND 35–38 (Rivest, 1991) and most male Sprague‐Dawley rats (∼90%) exhibiting puberty (as indexed by balano‐preputial separation) around PND 42 (Fuochi et al., 2022). We use adolescence to refer to animals that are postpubertal but have not reached adulthood (∼P60).…”

Section: Methodsmentioning

confidence: 99%

Behavioral responses to natural rewards in developing male and female rats

Oak,

Nguyen,

Rodney‐Hernández

et al. 2023

Developmental Psychobiology

View full text Add to dashboard Cite

Reward deficits are a hallmark feature of multiple psychiatric disorders and often recapitulated in rodent models useful for the study of psychiatric disorders, including those employing early life stress. Moreover, rodent studies have shown sex differences during adulthood in response to natural and drug rewards under normative conditions and in stress‐based rodent models. Yet, little is known about the development of reward‐related responses under normative conditions, including how these may differ in rats of both sexes during early development. Comparing reward‐related behavioral responses between developing male and female rats may be useful for understanding how these processes may be affected in rodent models relevant to psychiatric disorders. To this end, we tested behavioral responses to natural rewards in male and female rats using sucrose consumption, sweet palatable food intake and social play tests at two timepoints (peripuberty, adolescence). Our results suggest comparable responses to consummatory and social rewards in male and female rats during peripuberty and adolescence as no sex differences were found for sucrose preference, chocolate candy intake or a subset of play behaviors (dorsal contacts, pins). These findings suggest that sex differences in response to these natural rewards emerge and may be more robust during adulthood.

show abstract

“…The co-exposed group was orally given both IPD and CPS at the same aforementioned doses (1/20 LD 50 ) and route. All treatments were given until the onset of puberty at the 60th day PND [ 47 ]. Weekly body weights of all rats were recorded and used to calibrate dosage volumes.…”

Section: Methodsmentioning

confidence: 99%

Neurobehavioral Responses and Toxic Brain Reactions of Juvenile Rats Exposed to Iprodione and Chlorpyrifos, Alone and in a Mixture

Abd‐Elhakim

Sharkawy

Gharib

et al. 2023

Toxics

View full text Add to dashboard Cite

Herein, male juvenile rats (23th postnatal days (PND)) were exposed to chlorpyrifos (CPS) (7.5 mg/kg b.wt) and/or iprodione (IPD) (200 mg IPD /kg b.wt) until the onset of puberty (60th day PND). Our results demonstrated that IPD and/or CPS exposure considerably reduced locomotion and exploration. However, CPS single exposure induced anxiolytic effects. Yet, neither IPD nor IPD + CPS exposure significantly affected the anxiety index. Of note, IPD and/or CPS-exposed rats showed reduced swimming time. Moreover, IPD induced significant depression. Nonetheless, the CPS- and IPD + CPS-exposed rats showed reduced depression. The individual or concurrent IPD and CPS exposure significantly reduced TAC, NE, and AChE but increased MDA with the maximum alteration at the co-exposure. Moreover, many notable structural encephalopathic alterations were detected in IPD and/or CPS-exposed rat brain tissues. The IPD + CPS co-exposed rats revealed significantly more severe lesions with higher frequencies than the IPD or CPS-exposed ones. Conclusively, IPD exposure induced evident neurobehavioral alterations and toxic reactions in the brain tissues. IPD and CPS have different neurobehavioral effects, particularly regarding depression and anxiety. Hence, co-exposure to IPD and CPS resulted in fewer neurobehavioral aberrations relative to each exposure. Nevertheless, their simultaneous exposure resulted in more brain biochemistry and histological architecture disturbances.

show abstract

Puberty onset curve in CD (Sprague Dawley) and Long Evans outbred male rats

Cited by 9 publications

References 12 publications

Effect of Sex and Cross-Sex Hormone Treatment on Renal Monocarboxylate-Transporter Expression in Rats

Effect of Sex and Cross-Sex Hormone Treatment on Renal Monocarboxylate-Transporter Expression in Rats

Behavioral responses to natural rewards in developing male and female rats

Neurobehavioral Responses and Toxic Brain Reactions of Juvenile Rats Exposed to Iprodione and Chlorpyrifos, Alone and in a Mixture

Contact Info

Product

Resources

About