Public and individual idiotopes in the anti‐poly(Glu⁶⁰,Ala³⁰,Tyr¹⁰) response: analysis by monoclonal antibodies

Abstract

From BALB/c mice immunized with anti-GAT monoclonal antibody (mAb) G5, we have obtained anti-idiotypic mAb against individual (or private) idiotopes, expressed by G5 as well as anti-GAT mAb, that are heteroclitic because they recognize poly-(Glu50, Tyr50) (GT) better than poly(Glu60, Ala30, Tyr10) (GAT). From BALB/c mice immunized with BALB/c polyclonal anti-GAT antibodies, anti-idiotypic mAb directed against public idiotopes expressed following GAT immunization in all individuals of all mouse strains tested have been obtained. Nine anti-idiotypic mAb were studied in detail. One of these mAb recognizes only polyclonal anti-GAT antibodies; the other eight recognize polyclonal anti-GAT antibodies and anti-GAT mAb. The distribution of the structures recognized by the different anti-idiotypic mAb on a battery of 20 anti-GAT mAb allows definition of two families of public idiotopes.

Gene repertoire of the anti-poly(Glu60Ala30Tyr10) (GAT) immune response: comparison of VH, V kappa, and D regions used by anti-GAT antibodies and monoclonal antibodies produced after anti-idiotypic immunization.

“…When compared to HP-GAT G5, the idiotopes expressed by these different Ab's are not identical, since the inhibition curves were found to have different slopes. However, all the Ab's isolated expressed the same characteristic idiotopes as all the BALB/c HP-GATs studied (22). As expected, Ab3 inhibited only the binding of HP-Id22 to G5 (25).…”

“…Therefore, the VH and V1K genes described are optimal for the expression of the two idiotopes previously defined (22) 6 T A T C T A C T A C T 6 T 6 T C T A C T A T 6 6 T T A C T A C C A CA 6 T 6 A 6 6 C T A…”

“…At the idiotypic level the anti-GAT response is characterized by the expression of the public idiotypic specificity p.GAT defined by a xenogeneic antiserum (20,21) and expressed in all HP-GAT (15). More recently, two discrete idiotopes characteristic of the anti-GAT responses have been identified (22)(23)(24)(25).…”

Immune response against poly(Glu60, Ala30, Tyr10) (GAT): immunization with monoclonal anti‐idiotypic antibodies leads to the predominant stimulation of idiotypically similar immunoglobulins with anti‐GAT activity

“…Structures recognized by HP-ld20, HP-Id2,, HP-Id24 and HP-Id25 (HP-Id20 family) always segregate together as do specificities recognized by HP-Id22, HP-Id27, HP-Id28 and HP-IdZ9 (HP-Id22 family). BALBlc HP-GAT always express both families of idiotopes while C57BW6 and DBA/2 HP-GAT express either all idiotopes or only the idiotope(s) of the HP-ld20 family [12].…”

“…These results have been extended using eight syngeneic anti-Id monoclonal antibodies (HP-Id; mAb) prepared against BALB/c polyclonal anti-GAT antibodies. HP-Id define public idiotopes expressed by all anti-GAT antibodies from all strains of mice tested [12,131. The distribution of the corresponding idiotopes has been studied on 21 HP-GAT of different genetic origin.…”