Background: Carbon monoxide (CO) is a toxic gas that lacks discernible color or scent. Individuals may experience the impact of carbon monoxide (CO) emissions originating from a range of sources, such as gas stoves, water heaters, fireplaces, and motor vehicles. Objective: The objective of this study is to evaluate the perception and knowledge pertaining to symptoms, risk factors, causes, and preventative strategies of carbon monoxide (CO) poisoning among the general population in the Kingdom of Saudi Arabia (KSA). Subjects and methods: The present investigation constitutes a cross-sectional study conducted on the overall population of the Kingdom of Saudi Arabia (KSA). The research focused on those who are not involved in the healthcare profession, utilizing an electronic survey as the primary data collection method. The questionnaire primarily comprises three sections: socio-demographic information, knowledge evaluation, and habitual factors and behaviours pertaining to carbon monoxide poisoning. Results: Out of 568 participants, (52.8%) were aged between 18 to 25 years with (64.1%) being females. A significant proportion of the participants exhibited little knowledge (67.6%), unfavorable attitudes (72.4%), but shown commendable adherence to preventive measures (64.4%). Conclusion: There was a lack of knowledge and a weakness in attitude. Nevertheless, preventive practices are highly motivating. Individuals who possess awareness of carbon monoxide (CO) poisoning tend to demonstrate superior levels of knowledge and adherence to safety precautions when compared to those who lack such awareness.