Public Health Hazards Associated With Naturally Occurring Toxins in Legume Seeds - Rapid Detection and Characterization of a Lectin From a Korean Cultivated Kidney Bean (Phaseolus Vulgaris L.)

Abstract

Objective: The delicacy of kidney beans is highly appreciable but, at the same time, their toxicity has raised an alarming concern. In this regard, more public awareness is needed about bean poisoning.Methods: This work aimed at promptly investigating the chemical and biological properties of a lectin derived from a Korean cultivar of the common bean (Phaseolus vulgaris L.).Results: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed the presence of two major polypeptide bands around 31.00 kDa. Hemagglutination assay and Ouchterlony double immunodiffusion technique confirmed the presence of lectin in dry seeds.Conclusion: It was evident that the Korean bean cultivar contains biologically active lectins, which may pose a risk to the consumer if the beans are eaten in raw or undercooked state.

EFFECT OF GERMINATION AND COOKING ON IRON CONTENT, PHYTIC ACID AND LECTINS OF FOUR VARIETIES OF CHILEAN BEANS ( Phaseolus Vulgaris )

“…Lectins, also called phytohematoagglutinins, are glycoproteins that have high selectivity towards carbohydrates and can agglutinate red blood cells. Its adverse effects described are related to a reduction in growth and weight loss in laboratory animals, associated with the reduction in the absorption of digestion products in the small intestine [6], [9], [10]. On the other hand, these non-nutritional components have shown anti-tumour and anti-proliferative effects in colorectal cancer cell lines [11].…”

Legume Indian Bean (Phaseolus Vulgaris) Partially Purified Anti-Nutritional Protein Factor Exhibit Anti-Oxidant, Immunomodulatory and Anti-Cancer Properties

“…Cassava is one of the plants that contain high amounts of cyanides, it is a poisonous respiratory compound [4]. Intake of these plant food products in their fresh form is accepted to contain the highest micronutrients than in their processed form; this has turned ought to be a major health complication because of the occurrence of high profile-ANFs and toxic substances [5]. ANFs can be portioned into two groups; namely heat-stable ANFs contains tannins, phytic acids, alkaloids, non-protein amino acids, saponins, etc., and heat-labile ANFs contain lectins, protease inhibitors, cyanogen glycosides, and toxic amino acids, etc.…”

Detection of Lectin Protein Allergen of Kidney Beans (Phaseolus vulgaris L.) and Desensitization Food Processing Technology

“…147 The double immunodiffusion (Ouchterlony method) can also characterize the reaction between lectin and rabbit antilectin antibody, because a precipitation line would be formed on the gel plate when the antigen and antibody meet and reach the optimal ratio. 19,148 The RIEP method is an antigen detection method that combines electrophoresis and (oneway) immunodiffusion. During electrophoresis, the action of the electric field moves the lectin antigen to the positive electrode, and the antigen and antibody molecules form a rocket-shaped antigen−antibody immunoprecipitation peak.…”