China is one of the biggest consumers of genetically modified (GM) products, importing maize, soybeans and canola, and producing GM cotton. The cultivation of GM food crop, however, is still not permitted. Many studies argue that consumers’ attitude toward GM food safety is a major barrier to GM food crop production in China. Recent studies suggest that special interest groups such as biotechnology scientists with an economic interest in biotechnology are more supportive of the technology than groups with nothing to gain. Others believe that agribusiness groups influence the debate about GM food production. This is the first study that examined agribusiness managers’ attitudes toward GM biosafety and their support for GM crop production. The sample was 160 firms in the seed, pesticide, feed and food processing industries. We found most agribusiness leaders are concerned about GM food consumption and oppose GM crop production. Using regression models, we found business managers’ attitudes toward GM crop cultivation are more supportive if they expect to profit, if they are already using GM crops in their firm or are doing research on GM crops.