Publication-based analysis of miR-210 dependent biomarkers of pre-eclampsia

Abstract

MicroRNAs (miRNAs) are potential biomarkers of most pregnancy complications. In recent years, miR-210 has been shown as one of the main biomarkers, detected at different stages of pregnancy and associated with various diseases, including pre-eclampsia (PE). However, miR-210 is not reported as a marker of PE in about half of the studies. We filtered available published RNA-seq data and miRNAs associated with PE, including or excluding miR-210, obtained from the PregMiR database. For further analysis we only considered miRNAs appearing in at least four different studies. We observed that miR-152, miR-1 and miR-193b were only detected in studies with a changed miR-210 level, whereas miR-27a, miR-29a, miR-130a and miR-519b were detected in studies without miRNA-210 differential expression. Common biomarkers of PE are miR-182, miR-126, miR-155, miR-181a, miR-18a, miR-195, miR-21, miR-223, miR-335, miR-517c, miR-518b, miR-518e and let-7f. Based on the obtained data and taking into account the direction of differential miRNA expression, it can be assumed that the most likely mechanisms of PE development in the early pregnancy stage are either upregulation of miR-210, miR-152, miR-518b and downregulation of miR-126; or upregulation of miR-126 and downregulation of miR-182 and miR-518b. Late stages of PE are determined by miR-210, miR-152, miR-518b, miR-21, miR-155, miR-181a, miR-182, miR-193b-3p, miR-517c, miR-518e (upregulation) and miR-126, miR-18a, miR-195, miR-223, let-7f (downregulation); or miR-27a, miR-29a, miR-130a and miR-519d, miR-517c, miR-518e miR-155, miR-126, miR-181a, miR-195 (upregulation) and miR-223, miR-18a, miR-182 (downregulation). The presented results allow speculation about the influence of certain miRNAs on PE development in the context of the presence or absence of miR-210 differential expression, but additional experimental studies are required to evaluate the findings.

Association of Circulating miRNA Expression with Preeclampsia, Its Onset, and Severity

“…Based on our comparison of expression level between mild and severe PE and early and late-onset PE subtypes, expression of miR-210 did not vary significantly between these subgroups; hence, we can not assume that level of circulating miR-210 could distinguish PE subtypes from each other. The publication-based analysis provided by Tkachenko et al (2020) showed that there could be several different mechanisms of PE development, according to the detection of dysregulated miRNAs, and not all of them are associated with miR-210 upregulation [ 40 ].…”

Exploring the role of exosomal MicroRNAs as potential biomarkers in preeclampsia

“…Tkachenko et al. assumed that the altered level of miR-210 could influence the expression of specific miRNAs in the development of PE, including miR-1, miR-27a, miR-29a, miR-130a, miR-152, miR-193b, and miR-519b ( 16 ). Escudero et al.…”

“…Notably, exosomal microRNAs (exomiRs) have also been implicated in PE, including miR-153-3p and miR-517a (8). Tkachenko et al assumed that the altered level of miR-210 could influence the expression of specific miRNAs in the development of PE, including miR-1, miR-27a, miR-29a, miR-130a, miR-152, miR-193b, and miR-519b (16). Escudero et al proposed that the synthesis of exosomes containing miRNAs is influenced by various factors such as hypoxia and the balance between pro-oxidative and antioxidative mechanisms, and they may contribute to the significant changes in endothelial protein expression observed in PE, resulting in endothelial dysfunction in both maternal and maternal-fetal circulation and subsequent impairment of angiogenesis, which is the key feature of PE (1).…”

High-Throughput Sequencing of Circulating MicroRNAs in Plasma and Serum during Pregnancy Progression

“…MiRNAs are shown to be involved in regulation of pregnancy progression [4]. Differentially expressed miRNAs have been detected in the placenta at different gestational ages and in pregnancy complications in numerous studies [1,[4][5][6][7][8].…”