Publisher Correction: Elastomeric sensor surfaces for high-throughput single-cell force cytometry

Pushkarsky, Ivan; Tseng, Peter; Black, Dylan; Warfe, Lyndon; Koziol‐White, Cynthia; Jester, William F.; Trinh, Ryan; Lin, Jonathan; Scumpia, Philip O.; Morrison, Sherie L.; Panettieri, Reynold A.; Damoiseaux, Robert; Carlo, Dino Di

doi:10.1038/s41551-018-0207-0

Cited by 2 publications

(1 citation statement)

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“…For example, by locally patterning cell adhesive proteins to the cavity region of the nanovials, cells can be preferentially bound within the nanovials, protecting them from shear stress during more vigorous handling steps such as emulsification, fluorescent activated cell sorting (FACS), or delivery in vivo into tissue for therapeutic applications. Additionally, selective adhesion can direct loading to an adhesive region or cavity sized to the dimensions of a single cell, [14][15][16] improving loading statistics beyond random Poisson processes. While more scalable manufacturing devices such as parallelized step emulsifiers and highly parallelized flow focusing devices have been used to dramatically enhance the production rate of spherical microparticles, 3,[17][18][19] high-throughput production of shaped 3Dparticles or capsules which require two phases has not been achieved.…”

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confidence: 99%

Scalable Fabrication of 3D Structured Microparticles Using Induced Phase Separation

Lee

Rutte

Dimatteo

et al. 2021

Preprint

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Microparticles with defined shapes and spatial chemical modification can enable new opportunities to interface with cells and tissues at the cellular scale. However, conventional methods to fabricate shaped microparticles have trade-offs between the throughput of manufacture and precision of particle shape and chemical functionalization. Here, we achieved scalable production of hydrogel microparticles at rates of greater than 40 million/hour with localized surface chemistry using a parallelized step emulsification device and temperature-induced phase-separation. The approach harnesses a polymerizable polyethylene glycol (PEG) and gelatin aqueous-two phase system (ATPS) which conditionally phase separates within microfluidically-generated droplets. Following droplet formation, phase separation is induced and phase separated droplets are subsequently crosslinked to form uniform crescent and hollow shell particles with gelatin functionalization on the boundary of the cavity. The gelatin localization enabled deterministic cell loading in nanoliter-sized crescent-shaped particles, which we refer to as nanovials, with cavity dimensions tuned to the size of cells. Loading on nanovials also imparted improved cell viability during analysis and sorting using standard fluorescence activated cell sorters, presumably by protecting cells from shear stress. This localization effect was further exploited to selectively functionalize capture antibodies to nanovial cavities enabling single-cell secretion assays with reduced cross-talk in a simplified format.

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confidence: 99%

Scalable Fabrication of 3D Structured Microparticles Using Induced Phase Separation

Lee

Rutte

Dimatteo

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

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High extracellular glucose promotes cell motility by modulating cell deformability and contractility via the cAMP-RhoA-ROCK axis in human breast cancer cells

Batty

Banerjee

et al. 2023

MBoC

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The mechanical properties, or mechanotypes, of cells are largely determined by their deformability and contractility. The ability of cancer cells to deform and generate contractile force is critical in multiple steps of metastasis. Identifying soluble cues that regulate cancer cell mechanotypes and understanding the underlying molecular mechanisms regulating these cellular mechanotypes could provide novel therapeutic targets to prevent metastasis. Although a strong correlation between high glucose level and cancer metastasis has been demonstrated, the causality is not elucidated, and the underlying molecular mechanisms remain largely unknown. In this study, using novel high-throughput mechanotyping assays, we show that human breast cancer cells become less deformable and more contractile with increased extracellular glucose levels (>5 mM). These altered cell mechanotypes are due to increased F-actin rearrangement and non-muscle myosin II (NMII) activity. We identify the cAMP-RhoA-ROCK-NMII axis to play a major role in regulating cell mechanotypes at high extracellular glucose levels, whereas calcium and myosin light chain kinase (MLCK) are not required. The altered mechanotypes are also associated with increased cell migration and invasion. Our study identifies key components in breast cancer cells that convert high extracellular glucose levels into changes in cellular mechanotype and behaviors relevant in cancer metastasis.

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Publisher Correction: Elastomeric sensor surfaces for high-throughput single-cell force cytometry

Cited by 2 publications

References 0 publications

Scalable Fabrication of 3D Structured Microparticles Using Induced Phase Separation

Scalable Fabrication of 3D Structured Microparticles Using Induced Phase Separation

High extracellular glucose promotes cell motility by modulating cell deformability and contractility via the cAMP-RhoA-ROCK axis in human breast cancer cells

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