Publisher Correction: Migrasome formation is mediated by assembly of micron-scale tetraspanin macrodomains

Huang, Yuwei; Zucker, Ben; Zhang, Shaojin; Elias, Sharon; Zhu, Yun; Chen, Hui; Ding, Tianlun; Li, Ying; Sun, Yujie; Lou, Jizhong; Kozlov, Michael M.; Yu, Li

doi:10.1038/s41556-019-0389-z

Cited by 5 publications

(2 citation statements)

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“…Migrasomes are migrating cells derived large vesicles that contain small vesicles which are Tetraspanin (TSPAN) 4‐expressing membrane‐bound structures that bind to retraction fibers. [ 38 ] Retention of migrasomes in the lung of BM‐MSC transferred stroke models was documented for at least 3d after tMCAO, which, as far as we were concerned, were produced by migrating BM‐MSC when passing the lung.…”

Section: Discussionmentioning

confidence: 99%

Bone Marrow Mesenchymal Stem Cell‐Derived Dermcidin‐Containing Migrasomes enhance LC3‐Associated Phagocytosis of Pulmonary Macrophages and Protect against Post‐Stroke Pneumonia

Li,

Su,

et al. 2023

Advanced Science

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Pneumonia is one of the leading causes of death in patients with acute ischemic stroke (AIS). Antibiotics fail to improve prognosis of patientswith post-stroke pneumonia, albeit suppressing infection, due to adverse impacts on the immune system. The current study reports that bone marrow mesenchymal stem cells (BM-MSC) downregulate bacterial load in the lungs of stroke mice models. RNA-sequencing of the lung from BM-MSC-treated stroke models indicates that BM-MSC modulates pulmonary macrophage activities after cerebral ischemia. Mechanistically, BM-MSC promotes the bacterial phagocytosis of pulmonary macrophages through releasing migrasomes, which are migration-dependent extracellular vesicles. With liquid chromatography-tandem mass spectrometry (LC-MS/MS), the result shows that BM-MSC are found to load the antibacterial peptide dermcidin (DCD) in migrasomes upon bacterial stimulation. Besides the antibiotic effect, DCD enhances LC3-associated phagocytosis (LAP) of macrophages, facilitating their bacterial clearance. The data demonstrate that BM-MSC is a promising therapeutic candidate against post-stroke pneumonia, with dual functions of anti-infection and immunol modulation, which is more than a match for antibiotics treatment.

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Section: Discussionmentioning

confidence: 99%

Bone Marrow Mesenchymal Stem Cell‐Derived Dermcidin‐Containing Migrasomes enhance LC3‐Associated Phagocytosis of Pulmonary Macrophages and Protect against Post‐Stroke Pneumonia

Li,

Su,

et al. 2023

Advanced Science

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“…Nevertheless, the effective utilization of migrasomes as biomarkers has encountered obstacles owing to the lack of straightforward capture and analysis techniques. Traditional approaches for migrasome isolation and quantification involve time‐consuming and labour‐intensive methods, such as density gradient centrifugation for purification, electron microscopy and immunofluorescence microscopy for quantification, and Western blotting or qRT‐PCR for the analysis of migrasome‐contained proteins and RNAs (Baumann, 2021; Chen et al., 2018; Cheng et al., 2022; Ding et al., 2023; Fan et al., 2022; Huang et al., 2019; Jiang et al., 2019; Liang & Yu, 2023; Liu et al., 2020; Lu et al., 2020; Ma et al., 2015; Qin et al., 2022; Saito et al., 2021; Schmidt‐Pogoda et al., 2018; Wu et al., 2022; Wu et al., 2017; Zhang et al., 2022; Zhao et al., 2019; Zhen & Stenmark, 2023; Zhu et al., 2021; Zhang et al 2022, Li et al, 2023). These methods are ill‐suited for clinical application due to their demands for substantial sample volumes, complexity, limited throughput, high costs and extended processing times.…”

Section: Introductionmentioning

confidence: 99%

Quantification of urinary podocyte‐derived migrasomes for the diagnosis of kidney disease

Yang,

Zhang,

Chen

et al. 2024

J of Extracellular Vesicle

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Migrasomes represent a recently uncovered category of extracellular microvesicles, spanning a diameter range of 500 to 3000 nm. They are emitted by migrating cells and harbour a diverse array of RNAs and proteins. Migrasomes can be readily identified in bodily fluids like serum and urine, rendering them a valuable non‐invasive source for disease diagnosis through liquid biopsy. In this investigation, we introduce a streamlined and effective approach for the capture and quantitative assessment of migrasomes, employing wheat germ agglutinin (WGA)‐coated magnetic beads and flow cytometry (referred to as WBFC). Subsequently, we examined the levels of migrasomes in the urine of kidney disease (KD) patients with podocyte injury and healthy volunteers using WBFC. The outcomes unveiled a substantial increase in urinary podocyte‐derived migrasome concentrations among individuals with KD with podocyte injury compared to the healthy counterparts. Notably, the urinary podocyte‐derived migrasomes were found to express an abundant quantity of phospholipase A2 receptor (PLA2R) proteins. The presence of PLA2R proteins in these migrasomes holds promise for serving as a natural antigen for the quantification of autoantibodies against PLA2R in the serum of patients afflicted by membranous nephropathy. Consequently, our study not only pioneers a novel technique for the isolation and quantification of migrasomes but also underscores the potential of urinary migrasomes as a promising biomarker for the early diagnosis of KD with podocyte injury.

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The roles of migrasomes in immunity, barriers, and diseases

Cai,

Shen

2024

Acta Biomaterialia

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Publisher Correction: Migrasome formation is mediated by assembly of micron-scale tetraspanin macrodomains

Cited by 5 publications

References 0 publications

Bone Marrow Mesenchymal Stem Cell‐Derived Dermcidin‐Containing Migrasomes enhance LC3‐Associated Phagocytosis of Pulmonary Macrophages and Protect against Post‐Stroke Pneumonia

Bone Marrow Mesenchymal Stem Cell‐Derived Dermcidin‐Containing Migrasomes enhance LC3‐Associated Phagocytosis of Pulmonary Macrophages and Protect against Post‐Stroke Pneumonia

Quantification of urinary podocyte‐derived migrasomes for the diagnosis of kidney disease

The roles of migrasomes in immunity, barriers, and diseases

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