Publisher Correction: The impact of genetic diversity on gene essentiality within the Escherichia coli species

Rousset, François; Cabezas-Caballero, Jose; Piastra-Facon, Florence; Fernández-Rodríguez, Jesús; Clermont, Olivier; Denamur, Erick; Rocha, Eduardo P. C.; Bikard, David

doi:10.1038/s41564-021-00893-0

Cited by 2 publications

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“…Recently, gene essentiality in 18 E. coli strains, including UTI89, was assessed and compared during growth in LB, M9-glucose and gut microbiota media using a novel CRISPR interference screening platform [ 50 ]. A total of 340 essential genes for growth in LB in UTI89 were identified, which disagrees with the current study (73 genes detected).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide analysis of fitness-factors in uropathogenic Escherichia coli during growth in laboratory media and during urinary tract infections

et al. 2021

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Uropathogenic Escherichia coli (UPEC) UTI89 is a well-characterized strain, which has mainly been used to study UPEC virulence during urinary tract infection (UTI). However, little is known on UTI89 key fitness-factors during growth in lab media and during UTI. Here, we used a transposon-insertion-sequencing approach (TraDIS) to reveal the UTI89 essential-genes for in vitro growth and fitness-gene-sets for growth in Luria broth (LB) and EZ-MOPS medium without glucose, as well as for human bacteriuria and mouse cystitis. A total of 293 essential genes for growth were identified and the set of fitness-genes was shown to differ depending on the growth media. A modified, previously validated UTI murine model, with administration of glucose prior to infection was applied. Selected fitness-genes for growth in urine and mouse-bladder colonization were validated using deletion-mutants. Novel fitness-genes, such as tusA, corA and rfaG; involved in sulphur-acquisition, magnesium-uptake, and LPS-biosynthesis, were proved to be important during UTI. Moreover, rfaG was confirmed as relevant in both niches, and therefore it may represent a target for novel UTI-treatment/prevention strategies.

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Section: Discussionmentioning

confidence: 99%