Publisher's Note: Voltage-dependent capacitance of human embryonic kidney cells [Phys. Rev. E<b>73</b>, 041930 (2006)]

Farrell, Brenda; Shope, Cythnia Do; Brownell, William E.

doi:10.1103/physreve.73.059901

Cited by 6 publications

(7 citation statements)

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“…C M increases by 0.64% at ±100 mV (β = 0.64 V –2 ) in the example in Figure c, and therefore accounts for ∼10% of the overall increase. While smaller in magnitude, this quadratic voltage dependent C M increase is consistent with observations of BLMs formed in decane (Δ C M = ∼0.02 μFcm –2 at ±100 mV or β = ∼4 V –2 ). ,,,,,, Interestingly, Farrell et al measure a much smaller response in whole cell patch clamping experiments on HEK cells (β = ∼0.1 V –2 ) . In multiple experiments, we observe a small range of β from 0.6 to 1.5 V –2 (Figure c and Table S2, Supporting Information).…”

Section: Resultssupporting

confidence: 89%

“…12,17,19,20,30,32,33 Interestingly, Farrell et al measure a much smaller response in whole cell patch clamping experiments on HEK cells (β = ∼0.1 V À2 ). 56 In multiple experiments, we observe a small range of β from 0.6 to 1.5 V À2 (Figure 4c and Table S2, Supporting Information). Considering eqs 1, 3, 4, and 6, which give a = β + b, we find that the specific capacitance changes described by account for ∼5À 13% of the overall C T increase.…”

Section: Effect Of Applied Potential On Specific Capacitance (C M )mentioning

confidence: 88%

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Determining Membrane Capacitance by Dynamic Control of Droplet Interface Bilayer Area

et al. 2011

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By making dynamic changes to the area of a droplet interface bilayer (DIB), we are able to measure the specific capacitance of lipid bilayers with improved accuracy and precision over existing methods. The dependence of membrane specific capacitance on the chain-length of the alkane oil present in the bilayer is similar to that observed in black lipid membranes. In contrast to conventional artificial bilayers, DIBs are not confined by an aperture, which enables us to determine that the dependence of whole bilayer capacitance on applied potential is predominantly a result of a spontaneous increase in bilayer area. This area change arises from the creation of new bilayer at the three phase interface and is driven by changes in surface tension with applied potential that can be described by the Young-Lippmann equation. By accounting for this area change, we are able to determine the proportion of the capacitance dependence that arises from a change in specific capacitance with applied potential. This method provides a new tool with which to investigate the vertical compression of the bilayer and understand the changes in bilayer thickness with applied potential. We find that, for 1,2-diphytanoyl-sn-glycero-3-phosphocholine membranes in hexadecane, specific bilayer capacitance varies by 0.6-1.5% over an applied potential of ±100 mV.

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Section: Resultssupporting

confidence: 89%

Section: Effect Of Applied Potential On Specific Capacitance (C M )mentioning

confidence: 88%

Determining Membrane Capacitance by Dynamic Control of Droplet Interface Bilayer Area

et al. 2011

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“…Human embryonic kidney cells display an offset potential of À51 mV. 34 This indicates that the capacitance in electrophysiological models such as the Hodgkin-Huxley model 1 is incorrectly used because offset potentials are not considered. However, it is very likely that the offset potentials are closely related to the resting potentials of membranes.…”

Section: Summary and Discussionmentioning

confidence: 99%

Mechano-capacitive properties of polarized membranes

2015

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Biological membranes are capacitors that can be charged by applying a field across the membrane. The charges on the capacitor exert a force on the membrane that leads to electrostriction, i.e. a thinning of the membrane. Since the force is quadratic in voltage, negative and positive voltage have an identical influence on the physics of symmetric membranes. However, this is not the case for a membrane with an asymmetry leading to a permanent electric polarization. Positive and negative voltages of identical magnitude lead to different properties. Such an asymmetry can originate from a lipid composition that is different on the two monolayers of the membrane, or from membrane curvature. The latter effect is called 'flexoelectricity'. As a consequence of permanent polarization, the membrane capacitor is discharged at a voltage different from zero. This leads to interesting electrical phenomena such as outward or inward rectification of membrane permeability. Here, we introduce a generalized theoretical framework, that treats capacitance, polarization, flexoelectricity, piezoelectricity and thermoelectricity in the same language. We show applications to electrostriction, membrane permeability and piezoelectricity and thermoelectricity close to melting transitions, where such effects are especially pronounced.

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“…Patch pipettes (quartz glass) with resistances ranging from 2 to 4 megohms were fabricated using a laser-based micropipette puller (P-2000, Sutter Instrument Company, Novato, CA) and filled with an intracellular solution, also containing channel blockers. For cell membrane admittance, Y was measured with the patch-clamp technique in the whole-cell mode using a DC voltage ramp with dual frequency stimulus (62) from Ϫ0.14 to 0.14 V with a holding potential of 0 V, and the cell parameters were calculated from the admittance as described earlier (63). The conductance, b, was also determined experimentally with a DC protocol, as described earlier (60).…”

Section: Electrophysiological Measurementsmentioning

confidence: 99%

Tuning of the Outer Hair Cell Motor by Membrane Cholesterol

Rajagopalan

Greeson

Xia³

et al. 2007

Journal of Biological Chemistry

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134

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Cholesterol affects diverse biological processes, in many cases by modulating the function of integral membrane proteins. We observed that alterations of cochlear cholesterol modulate hearing in mice. Mammalian hearing is powered by outer hair cell (OHC) electromotility, a membrane-based motor mechanism that resides in the OHC lateral wall. We show that membrane cholesterol decreases during maturation of OHCs. To study the effects of cholesterol on hearing at the molecular level, we altered cholesterol levels in the OHC wall, which contains the membrane protein prestin. We show a dynamic and reversible relationship between membrane cholesterol levels and voltage dependence of prestin-associated charge movement in both OHCs and prestin-transfected HEK 293 cells. Cholesterol levels also modulate the distribution of prestin within plasma membrane microdomains and affect prestin self-association in HEK 293 cells. These findings indicate that alterations in membrane cholesterol affect prestin function and functionally tune the outer hair cell.

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Publisher's Note: Voltage-dependent capacitance of human embryonic kidney cells [Phys. Rev. E73, 041930 (2006)]

Cited by 6 publications

References 0 publications

Determining Membrane Capacitance by Dynamic Control of Droplet Interface Bilayer Area

Determining Membrane Capacitance by Dynamic Control of Droplet Interface Bilayer Area

Mechano-capacitive properties of polarized membranes

Tuning of the Outer Hair Cell Motor by Membrane Cholesterol

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