Publishing and sharing multi-dimensional image data with OMERO

Burel, Jean‐Marie; Besson, Sébastien; Blackburn, Colin; Carroll, Mark; Ferguson, Gus; Flynn, Helen; Gillen, Kenneth; Leigh, R. A.; Li, Simon; Lindner, Dominik; Linkert, Melissa; Moore, William J.; Ramalingam, Balaji; Rozbicki, Emil; Tarkowska, Aleksandra; Walczysko, Petr; Allan, Chris; Moore, Josh; Swedlow, Jason R.

doi:10.1007/s00335-015-9587-6

Cited by 45 publications

(26 citation statements)

References 22 publications

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“…OMERO’s client–server architecture enables remote access to the data it holds. OMERO’s permissions system controls access to that data ensuring that each dataset, image, annotation or analytic result is only retrievable by those with correct permissions to do so [4] . A Python-based scripting engine provides an interface for data processing and supports processing and analysis applications.…”

Section: Methodsmentioning

confidence: 99%

Metadata management for high content screening in OMERO

Besson

Blackburn

et al. 2016

Methods

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Section: Methodsmentioning

confidence: 99%

Metadata management for high content screening in OMERO

Besson

Blackburn

et al. 2016

Methods

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“…Image acquisition was performed with the soft-WoRx suite (Applied Precision/GE Healthcare). Image post-processing for publication was limited to linear intensity scaling and maximumintensity projection using OMERO (Burel et al, 2015). The following antibodies used in the present study have been described previously: HTP-1 (Martinez-Perez et al, 2008), LAB-1(de Carvalho et al, 2008), PLK-2 (Labella et al, 2011), ICP-1 (Oegema et al, 2001), HIM-8(Phillips et al, 2005), HTP-3 (MacQueen et al, 2005), SUN-1:Ser8p (Penkner et al, 2009), SYP-1 (Harper et al, 2011), ZIM-3 (Phillips andDernburg, 2006)), phosphorylated SYP-4 (Nadarajan et al, 2017) andCOH-3/4 (Severson andMeyer, 2014).…”

Section: Microscopy Cytology Antibodiesmentioning

confidence: 99%

Phosphorylation of the synaptonemal complex protein SYP-1 promotes meiotic chromosome segregation

Sato-Carlton

Tabuchi

Chartrand

et al. 2017

Preprint

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Chromosomes that have undergone crossing-over in meiotic prophase must maintain sister chromatid cohesion somewhere along their length between the first and second meiotic divisions. While many eukaryotes use the centromere as a site to maintain cohesion, the holocentric organism C. elegans instead creates two chromosome domains of unequal length termed the short arm and long arm, which become the first and second site of cohesion loss at meiosis I and II. The mechanisms that confer distinct functions to the short and long arm domains remain poorly understood. Here, we show that phosphorylation of the synaptonemal complex protein SYP-1 is required to create these domains. Once crossovers are made, phosphorylated SYP-1 and PLK-2 become cooperatively confined to short arms and guide phosphorylated histone H3 and the chromosomal passenger complex to the site of meiosis I cohesion loss. Our results show that PLK-2 and phosphorylated SYP-1 ensure creation of the short arm subdomain, promoting disjunction of chromosomes in meiosis I.

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“…The following statistical tests were performed using Prism 8 (GraphPad): Student's t-tests; one-way or two-way Anova, with Tukey correction for multiple comparisons; multiple linear regression with least squares. Figures were made using FigureApp in OMERO (Allan et al, 2012;Burel et al, 2015) and final assembly in Adobe Photoshop.…”

Section: Generation Of Mosaic Clones Using Marcm Mosaicmentioning

confidence: 99%

DrosophilaUSP22/non-stop regulates the Hippo pathway to polarise the actin cytoskeleton during collective border cell migration

Badmos

Cobbe

Campbell

et al. 2020

Preprint

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Polarisation of the actin cytoskeleton is vital for the collective migration of cells in vivo. During invasive border cell migration in Drosophila, actin polarisation is directly controlled by Hippo pathway components, which reside at contacts between border cells in the cluster. Here we identify, in a genetic screen for deubiquitinating enzymes involved in border cell migration, an essential role for non-stop/USP22 in the expression of Hippo pathway components expanded and merlin; loss of non-stop function consequently leads to a redistribution of F-actin and the polarity determinant Crumbs, loss of polarised actin protrusions and premature tumbling of the border cell cluster. Non-stop is a component of the Spt-Ada-Gcn5-acetyltransferase (SAGA) transcriptional coactivator complex, but SAGA’s histone acetyltransferase module, which does not bind to expanded or merlin, is dispensable for migration. Taken together, our results uncover novel roles for SAGA-independent non-stop/USP22 in Hippo-mediated collective cell migration, which may help guide studies in other systems where USP22 is necessary for cell motility and invasion.

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Publishing and sharing multi-dimensional image data with OMERO

Cited by 45 publications

References 22 publications

Metadata management for high content screening in OMERO

Metadata management for high content screening in OMERO

Phosphorylation of the synaptonemal complex protein SYP-1 promotes meiotic chromosome segregation

DrosophilaUSP22/non-stop regulates the Hippo pathway to polarise the actin cytoskeleton during collective border cell migration

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