Puccinia spegazzinii (Pucciniales: Pucciniaceae) from Peru for biological control of Mikania micrantha (Asteraceae: Eupatorieae) in India: evaluating susceptibility of host populations and confirming host specificity

Kumar, Prabhat; Dev, Usha; Joshi, Nidhi

doi:10.1186/s41938-017-0024-x

Cited by 3 publications

(2 citation statements)

References 4 publications

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“…Puccinia spegazzinii is currently being used as an effective biological control agent and has been released against M. micrantha in many countries [ 1 , 7 , 11 , 58 , 59 ]. The management of M. micrantha using P. spegazzinii is seen as a benefit to many landholders, as plants can reshoot after slashing, and herbicides are harmful to humans and the environment [ 1 , 11 , 60 ].…”

Section: Discussionmentioning

confidence: 99%

Metabolomics and Transcriptomics Reveal the Response Mechanisms of Mikania micrantha to Puccinia spegazzinii Infection

et al. 2023

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Mikania micrantha is one of the 100 worst invasive species globally and can cause significant negative impacts on agricultural and forestry economics, particularly in Asia and the Pacific region. The rust Puccinia spegazzinii has been used successfully as a biological control agent in several countries to help manage M. micrantha. However, the response mechanisms of M. micrantha to P. spegazzinii infection have never been studied. To investigate the response of M. micrantha to infection by P. spegazzinii, an integrated analysis of metabolomics and transcriptomics was performed. The levels of 74 metabolites, including organic acids, amino acids, and secondary metabolites in M. micrantha infected with P. spegazzinii, were significantly different compared to those in plants that were not infected. After P. spegazzinii infection, the expression of the TCA cycle gene was significantly induced to participate in energy biosynthesis and produce more ATP. The content of most amino acids, such as L-isoleucine, L-tryptophan and L-citrulline, increased. In addition, phytoalexins, such as maackiain, nobiletin, vasicin, arachidonic acid, and JA-Ile, accumulated in M. micrantha. A total of 4978 differentially expressed genes were identified in M. micrantha infected by P. spegazzinii. Many key genes of M. micrantha in the PTI (pattern-triggered immunity) and ETI (effector-triggered immunity) pathways showed significantly higher expression under P. spegazzinii infection. Through these reactions, M. micrantha is able to resist the infection of P. spegazzinii and maintain its growth. These results are helpful for us to understand the changes in metabolites and gene expression in M. micrantha after being infected by P. spegazzinii. Our results can provide a theoretical basis for weakening the defense response of M. micrantha to P. spegazzinii, and for P. spegazzinii as a long-term biological control agent of M. micrantha.

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Section: Discussionmentioning

confidence: 99%

Metabolomics and Transcriptomics Reveal the Response Mechanisms of Mikania micrantha to Puccinia spegazzinii Infection

et al. 2023

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“…Puccinia spegazzinii is currently being used as an effective biological control agent and has been released against M. micrantha in many countries (Ellison et al, 2008;Winston et al, 2014;Day et al, 2016;Kumar et al, 2018;Day & Riding, 2019). The management of M. micrantha using P. spegazzinii is seen as a benefit to many land holders, as plants can reshoot after slashing and herbicides are harmful to humans and the environment (Choudhury, 1972;Day et al, 2016;Day & Riding, 2019).…”

Section: Discussionmentioning

confidence: 99%

Metabolomics and transcriptomics reveal the response mechanisms of Mikania micrantha to Puccinia spegazzinii infection

Liu

Ren

Zhang

et al. 2022

Preprint

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Mikania micrantha is one of the 100 worst invasive species globally and can cause significant negative impacts to agricultural and forestry economics, particularly in Asia and the Pacific region. The rust Puccinia spegazzinii has been used successfully as a biological control agent in several countries to help manage M. micrantha. However, the response mechanisms of M. micrantha to P. spegazzinii infection have never been studied. To investigate the response of M. micrantha to infection by P. spegazzinii, an integrated analysis of metabolomics and transcriptomics was performed. The levels of 101 metabolites, including organic acids, amino acids and secondary metabolites in M. micrantha infected with P. spegazzinii, were significantly different compared to those in plants that were not infected. The relative amounts of L-leucine, L-ioleucine, L-tryptophan, histidine, L-phenylalanine and L-citrulline were increased under P. spegazzinii infection. These may convert to intermediates within the TCA cycle, participating in energy biosynthesis. In addition, phytoalexins, such as maackiain, nobiletin, vasicin, arachidonic acid and JA-Ile, were synthesized and accumulated in M. micrantha in response to P. spegazzinii infection. A total of 4,978 differentially expressed genes were identified in M. micrantha infected by P. spegazzinii. Many key genes of the Mitogen-activated protein kinase (MAPK) signalling pathway, showed significantly higher expression under P. spegazzinii infection. These results not only help us to understand the response of metabolites and gene expression in M. micrantha following infection by P. spegazzinii, but also help us understand the potential for the continuous biological control of M. micrantha using P. spegazzinii.

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Evaluation of the short-term and long-term performance of biological invasion management in the China-Myanmar border region

Zhang

Huang

et al. 2019

Journal of Environmental Management

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Puccinia spegazzinii (Pucciniales: Pucciniaceae) from Peru for biological control of Mikania micrantha (Asteraceae: Eupatorieae) in India: evaluating susceptibility of host populations and confirming host specificity

Cited by 3 publications

References 4 publications

Metabolomics and Transcriptomics Reveal the Response Mechanisms of Mikania micrantha to Puccinia spegazzinii Infection

Metabolomics and Transcriptomics Reveal the Response Mechanisms of Mikania micrantha to Puccinia spegazzinii Infection

Metabolomics and transcriptomics reveal the response mechanisms of Mikania micrantha to Puccinia spegazzinii infection

Evaluation of the short-term and long-term performance of biological invasion management in the China-Myanmar border region

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