Puerarin alleviates cadmium‐induced oxidative damage to bone by reducing autophagy in rats

Abstract

Autophagy is a regulatory mechanism involved in cadmium (Cd)-induced bone toxicity and is suppressed by various stimuli, including oxidative stress. Puerarin is an isoflavonoid compound isolated from Pueraria, a plant used in traditional Chinese medicine. The underlying mechanisms of action of puerarin remain unclear. The objective of this study was to explore the mitigating effects of puerarin on cadmiuminduced oxidative damage in the bones of rats. Cadmium exposure increased oxidative damage in rat bones; this was markedly decreased by puerarin treatment, as demonstrated by changes in the activity of antioxidative enzymes. Cadmium-induced blockage of the expression of key bone regulatory proteins, autophagy-related markers, and signaling molecules was also alleviated by puerarin treatment. Additionally, cadmium reduced expression of the autophagic protein Rab7 and of late endosomal/lysosomal adaptor and MAPK and mTOR activator 1 (LAMTOR1); the decrease in these proteins was not restored by puerarin treatment. We speculate that puerarin relieves the inhibition of fusion of autophagosomes with lysosomes that is induced by cadmium; however, this specific effect of puerarin and downstream effects on bone regulatory mechanisms require further investigation. In conclusion, puerarin alleviates cadmium-induced oxidative damage in the bones of rats by attenuating autophagy, which is likely associated with the antioxidant activity of puerarin.

Polystyrene exacerbates cadmium‐induced mitochondrial damage to lung by blocking autophagy in mice

“…Cadmium leads to lung damage in animals. The oxidative enzymes are essential indicators for lung metabolic regulation, including MDA and T‐SOD, and also important prompter of Cd toxicity 38,42 . The lung coefficient of mice was analyzed, and it was found that the lung coefficient was decreased treatment with Cd compared with Con group (Figure 1A).…”

“…The lung tissue samples were dried at high temperature (80°C) for 24 h as previously described 38 . Samples were transferred into microwave digested tube for further analysis using a Microwave Digestion System (Anton Paar Trading Company, Shanghai, China).…”

Morinda officinalis Polysaccharides Ameliorates Bone Growth by Attenuating Oxidative Stress and Regulating the Gut Microbiota in Thiram-Induced Tibial Dyschondroplasia Chickens

“…TD is a susceptible disease of the tibial tarsus affecting the proximal growth plate of the tibia in fast-growing poultry, and its prevention and treatment have not been well defined. Numerous studies have confirmed that traditional Chinese herbal medicine plays an important role in improving bone development, such as total flavonoids of Rhizome drynariae [ 37 ], Puerarin [ 38 , 39 ], and Astragalus membranaceus [ 18 ]. We investigated the therapeutic effects of MOP on TD broilers, and the results showed that MOP could improve tibia-related indicators through BMP/Smads signaling pathway, improve the disturbance of Ca and P metabolism caused by TD, and increase the antioxidant capacity of the chickens.…”

Co‐exposure to molybdenum and cadmium triggers pyroptosis and autophagy byPI3K/AKTaxis in duck spleens

“…Excess generation of ROS can cause oxidative stress 39,40 . To assess whether the body is in a state of oxidative stress, usually detect some oxidative stress indicators, such as T‐AOC, GSH‐Px, T‐SOD and CAT activities and MDA level 41 . T‐AOC value signifies total antioxidant ability of cells.…”

“…39,40 To assess whether the body is in a state of oxidative stress, usually detect some oxidative stress indicators, such as T-AOC, GSH-Px, T-SOD and CAT activities and MDA level. 41 T-AOC value signifies total antioxidant ability of cells. T-SOD is a key player in preserving body's oxidative and antioxidant equilibrium by destroying superoxide radicals.…”