Puerarin alleviates cadmium-induced rat neurocyte injury by alleviating Nrf2-mediated oxidative stress and inhibiting mitochondrial unfolded protein response

Zhang, Wenhua; Xu, Mingchang; Wen, Sicheng; Wang, Li; Zhang, Kanglei; Zhang, Chaofan; Zou, Hui; Gu, Jianhong; Bian, Jianchun; Liu, Zongping; Yuan, Yan

doi:10.1016/j.ecoenv.2022.114239

Cited by 10 publications

(3 citation statements)

References 48 publications

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“…It is commonly used as a soup ingredient and can also be processed into various products such as Pueraria powder, noodles, and tea. In addition, P. thomsonii contained in Puerarin is a flavonoid compound that has been shown to protect the cardio-cerebrovascular system [2,3], reduce blood sugar [4], increase antioxidants [5], provide liver protection [6], fight cancer [7,8], and serve as a neuroprotective agent [9,10]. used to evaluate the expression patterns of the genes involved in delaying the PPD of P. thomsonii under ethephon treatment.…”

Section: Introductionmentioning

confidence: 99%

The Genome-Wide Identification of Stable Internal Reference Genes Related to Delayed Spoilage for Accurate qRT-PCR Normalization in Ethephon-Treated Pueraria thomsonii Benth.

Liu,

Li,

et al. 2023

Horticulturae

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Pueraria thomsonii Benth. is a perennial leguminous vine with medicinal and nutritional value. However, rapid postharvest physiological deterioration (PPD) reduces its quality and market value. To detect gene expression levels, the quantitative reverse transcription polymerase chain reaction (qRT-PCR) technique requires stable internal reference genes (IRGs). Our findings indicated that an ethephon (C2H6ClO3P) treatment delayed PPD in P. thomsonii tuberous roots and an RNA-seq analysis revealed a significant number of differentially expressed genes (DEGs). To find stable IRGs for the further identification of the genes associated with delayed PPD in P. thomsonii, eight candidate IRGs of the tuberous roots were screened and assessed using qRT-PCR. The expression stability of these genes was determined and ranked using five different algorithms, including NormFinder, BestKeeper, ΔCt, GeNorm, and ReFinder. Consequently, we identified two genes, PtUBC10 and PtACT7, as the best candidate IRGs for qRT-PCR normalization in P. thomsonii, both exposed to ethephon treatment and in different tissues. Moreover, PtUBC10 was found to be the most stably expressed IRG of P. thomsonii during the ethephon treatment. The findings of this investigation furnish significant insights for future gene expression analyses concerning the delay of PPD via ethephon administration, which could also be used in other tuberous plants.

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Section: Introductionmentioning

confidence: 99%

The Genome-Wide Identification of Stable Internal Reference Genes Related to Delayed Spoilage for Accurate qRT-PCR Normalization in Ethephon-Treated Pueraria thomsonii Benth.

Liu,

Li,

et al. 2023

Horticulturae

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“…The transcription factor Nrf2 is a key core factor in the regulation of encoding genes for many antioxidant responses that are induced by fipronil and some other environmental oxidative stressors. , Environmental pollutants as stressors can activate the body’s redox system and disrupt the intrinsic homeostatic balance . Under a state of oxidative stress, Nrf2 is dissociated, released, and translocated from the cytosol to the nucleus .…”

Section: Discussionmentioning

confidence: 99%

Thyroid-Disrupting Effects of Exposure to Fipronil and Its Metabolites from Drinking Water Based on Human Thyroid Follicular Epithelial Nthy-ori 3-1 Cell Lines

Zhuang

Jiang

Chen

et al. 2023

Environ. Sci. Technol.

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Fipronil is a broad-spectrum insecticide used for plants and poultry. Owing to its widespread use, fipronil and its metabolites (fipronil sulfone, fipronil desulfinyl, and fipronil sulfide), termed FPM, can be frequently detected in drinking water and food. Fipronil can affect the thyroid function of animals, but the effects of FPM on the human thyroid remain unclear. We employed human thyroid follicular epithelial Nthy-ori 3-1 cells to examine combined cytotoxic responses, thyroid-related functional proteins including the sodium-iodide symporter (NIS), thyroid peroxidase (TPO), deiodinases I−III (DIO I−III), and the nuclear factor erythroid-derived factor 2−related factor 2 (NRF2) pathway induced by FPM of 1−1000-fold concentrations detected in school drinking water collected from a heavily contaminated area of the Huai River Basin. Thyroid-disrupting effects of FPM were evaluated by examining biomarkers of oxidative stress and thyroid function and tetraiodothyronine (T4) levels secreted by Nthy-ori 3-1 cells after FPM treatment. FPM activated the expression of NRF2, HO-1 (heme oxygenase 1), TPO, DIO I, and DIO II but inhibited NIS expression and increased the T4 level of thyrocytes, indicating that FPM can disrupt the function of human thyrocytes through oxidative pathways. Given the adverse impact of low FPM concentrations on human thyrocytes, supportive evidence from rodent studies, and the critical importance of thyroid hormones on development, the effects of FPM on the neurodevelopment and growth of children warrant priority attention.

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“…Bioactive substances that are present in nature generally have powerful physiological or pharmacological effects, such as curcumin [ 4 ], tocopherols [ 5 ], flavonoids [ 6 ], resveratrol [ 7 ], anthocyanins [ 8 ], puerarin [ 9 ], ferulic acid [ 10 ], and carvacrol [ 11 ], which are not essential for maintaining human growth and development but have significant health benefits [ 12 ]. Recent research has shown that bioactive substances have plenty of functions—such as antioxidant [ 13 ], anti-inflammatory [ 14 ], antibacterial [ 15 ], anticancer, and immunomodulatory [ 16 , 17 ] functions—and play an important role in health maintenance, disease prevention, and physiological function regulation.…”

Section: Introductionmentioning

confidence: 99%

Research Progress of Protein-Based Bioactive Substance Nanoparticles

Han

Liu

et al. 2023

Foods

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Bioactive substances exhibit various physiological activities—such as antimicrobial, antioxidant, and anticancer activities—and have great potential for application in food, pharmaceuticals, and nutraceuticals. However, the low solubility, chemical instability, and low bioavailability of bioactive substances limit their application in the food industry. Using nanotechnology to prepare protein nanoparticles to encapsulate and deliver active substances is a promising approach due to the abundance, biocompatibility, and biodegradability of proteins. Common protein-based nanocarriers include nano-emulsions, nano-gels, nanoparticles, and nano complexes. In this review, we give an overview of protein-based nanoparticle fabrication methods, highlighting their pros and cons. Additionally, we discuss the applications and current issues regarding the utilization of protein-based nanoparticles in the food industry. Finally, we provide perspectives on future development directions, with a focus on classifying bioactive substances and their functional properties.

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Puerarin alleviates cadmium-induced rat neurocyte injury by alleviating Nrf2-mediated oxidative stress and inhibiting mitochondrial unfolded protein response

Cited by 10 publications

References 48 publications

The Genome-Wide Identification of Stable Internal Reference Genes Related to Delayed Spoilage for Accurate qRT-PCR Normalization in Ethephon-Treated Pueraria thomsonii Benth.

The Genome-Wide Identification of Stable Internal Reference Genes Related to Delayed Spoilage for Accurate qRT-PCR Normalization in Ethephon-Treated Pueraria thomsonii Benth.

Thyroid-Disrupting Effects of Exposure to Fipronil and Its Metabolites from Drinking Water Based on Human Thyroid Follicular Epithelial Nthy-ori 3-1 Cell Lines

Research Progress of Protein-Based Bioactive Substance Nanoparticles

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