Puerarin attenuates cisplatin-induced apoptosis of hair cells through the mitochondrial apoptotic pathway

Xu, Bingqiang; Li, Juedan; Chen, Xiaolong; Kou, Mingqing

doi:10.1016/j.bbamcr.2021.119208

Cited by 15 publications

(3 citation statements)

References 50 publications

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“…PUE has been repeatedly shown to have anti-apoptotic effects [28][29][30]. In this study, we investigated whether PUE can reduce apoptosis in HK2 cells through the PI3K/AKT signaling pathway in vitro using COM.Initially, it was observed that p-PI3K and p-AKT exhibited a notable decrease in the COM group when compared to the control group.…”

Section: 1network Pharmacology Analysismentioning

confidence: 99%

Puerarin ameliorates apoptosis and inflammation induced by kidney stones through the PI3K/AKT pathway: Network pharmacology and experimental validation

Xu,

Liang,

Mao

et al. 2024

Preprint

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Puerarin(PUE), an isoflavonoid extracted from Pueraria root, has anti-apoptotic effects. The objective of this research is to examine the impact of PUE on renal apoptosis and inflammation resulting from renal calculi and to elucidate its mechanism.The approach of network pharmacology and molecular docking was employed to discover potential targets and pathways of PUE. An animal model of calcium oxalate crystal deposition by intraperitoneal injection of glyoxylate and a model of COM-induced human renal tubular epithelial cells (HK2) were used to investigate the pharmacological mechanisms of PUE against apoptosis and inflammation. We used hematoxylin-eosin (H&E) and Periodic Acid-Schiff staining (PAS) to assess the effect of PUE on crystal deposition and damage. The mechanism of PUE was elucidated and validated using Western blotting, histology, and immunohistochemical staining.Network pharmacology findings indicated that the PI3K/AKT pathway plays a crucial role in PUE. We experimentally demonstrate that PUE alleviated COM-induced changes in apoptotic proteins, increased inflammatory indicators and changes in oxidative stress-related indicators in HK2 cells by activating the PI3K/AKT pathway, reduced serum creatinine and urea nitrogen levels in mice caused by CaOx, alleviated crystal deposition and damage, and alleviated apoptosis, oxidative stress and inflammation.Puerarin attenuates renal apoptosis and inflammation caused by kidney stones through the PI3K/AKT pathway.

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Section: 1network Pharmacology Analysismentioning

confidence: 99%

Puerarin ameliorates apoptosis and inflammation induced by kidney stones through the PI3K/AKT pathway: Network pharmacology and experimental validation

Xu,

Liang,

Mao

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…It reduces cisplatin-associated loss of neuronal fibers and synapses and promotes HCs and SGNs survival by preventing mitochondrial apoptosis induced by ROS accumulation (He et al, 2022b). Puerarin, a traditional Chinese medicine extract, can also inhibit ROS overproduction, affect the Bcl-2 family and AKT level to regulate the mitochondrial apoptosis pathway and protect hair cells (Xu et al, 2022). Autophagy is also an important cellular pathway for antagonistic strategy of cisplatin ototoxicity.…”

Section: Inhibitors Of Cellular Pathwaymentioning

confidence: 99%

Cisplatin ototoxicity mechanism and antagonistic intervention strategy: a scope review

Zhang

Song

et al. 2023

Front. Cell. Neurosci.

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Cisplatin is a first-line chemotherapeutic agent in the treatment of malignant tumors with remarkable clinical effects and low cost. However, the ototoxicity and neurotoxicity of cisplatin greatly limit its clinical application. This article reviews the possible pathways and molecular mechanisms of cisplatin trafficking from peripheral blood into the inner ear, the toxic response of cisplatin to inner ear cells, as well as the cascade reactions leading to cell death. Moreover, this article highlights the latest research progress in cisplatin resistance mechanism and cisplatin ototoxicity. Two effective protective mechanisms, anti-apoptosis and mitophagy activation, and their interaction in the inner ear are discussed. Additionally, the current clinical preventive measures and novel therapeutic agents for cisplatin ototoxicity are described. Finally, this article also forecasts the prospect of possible drug targets for mitigating cisplatin-induced ototoxicity. These include the use of antioxidants, inhibitors of transporter proteins, inhibitors of cellular pathways, combination drug delivery methods, and other mechanisms that have shown promise in preclinical studies. Further research is needed to evaluate the efficacy and safety of these approaches.

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“…The prevalence of sensorineural hearing loss (SNHL) is high worldwide, making it a common sensory deficit. The incidence of depression, dementia, stroke, and diabetes would exhibit a significant elevation in individuals with hearing impairment 1,2 . Sensory hair cells (HCs) are necessary for the transduction of mechanical stimulation into electrical signals in the inner ear.…”

Section: Introductionmentioning

confidence: 99%

Construction of organ of Corti organoid to study the effects of berberine sulfate on damaged auditory cells

Zhang,

Liu,

Shen

et al. 2024

J Biomed Mater Res

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Sensorineural hearing loss (SNHL) is mainly caused by injury or loss of hair cells (HCs) and associated spiral ganglion neurons (SGNs) in the inner ear. At present, there is still no effective treatment for SNHL in clinic. Recently, advances in organoid bring a promising prospect for research and treatment of SNHL. Meanwhile, three‐dimensional (3D) printing provides a tremendous opportunity to construct versatile organoids for tissue engineering and regenerative medicine. In this study, gelatin (Gel), sodium alginate (SA), and polyvinyl alcohol (PVA) were used to fabricate biomimetic scaffold through 3D printing. The organ of Corti derived from neonatal mice inner ear was seeded on the PVA/Gel/SA scaffold to construct organ of Corti organoid. Then, the organ of Corti organoid was used to study the potential protective effects of berberine sulfate on neomycin‐juried auditory HCs and SGNs. The results showed that the PVA/Gel/SA biomimetic 3D scaffolds had good cytocompatibilities and mechanical properties. The constructed organoid could maintain organ of Corti activity well in vitro. In addition, the injury intervention results showed that berberine sulfate could significantly inhibit neomycin‐induced HC and SGN damage. This study suggests that the fabricated organoid is highly biomimetic to the organ of Corti, which may provide an effective model for drug development, cell and gene therapy for SNHL.

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Puerarin attenuates cisplatin-induced apoptosis of hair cells through the mitochondrial apoptotic pathway

Cited by 15 publications

References 50 publications

Puerarin ameliorates apoptosis and inflammation induced by kidney stones through the PI3K/AKT pathway: Network pharmacology and experimental validation

Puerarin ameliorates apoptosis and inflammation induced by kidney stones through the PI3K/AKT pathway: Network pharmacology and experimental validation

Cisplatin ototoxicity mechanism and antagonistic intervention strategy: a scope review

Construction of organ of Corti organoid to study the effects of berberine sulfate on damaged auditory cells

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