Puerarin exerts antipyretic effect on lipopolysaccharide-induced fever in rats involving inhibition of pyrogen production from macrophages

Yao, Xiujuan; Yin, Ji-Ai; Xia, Yufeng; Wei, Zhifeng; Luo, Yubin; Liu, Mei; Feleder, Carlos; Dai, Yue

doi:10.1016/j.jep.2012.02.038

Cited by 44 publications

(27 citation statements)

References 44 publications

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“…A number of studies have revealed that puerarin possesses many biological activities, including the improvement of blood circulation [25], prevention of cardiovascular diseases [26], antipyretic [27], antioxidative [21] and antiallergic activities [22]. …”

Section: Discussionmentioning

confidence: 99%

“…As shown in Figures 5 and 6, test results indicated that puerarin could effectively enhance the activities of FII, FV, FVII, and FX and reduce the activities of FVIII, FIX, and FXII; these data elucidated the shortening of PT ( P < 0.01) and TT ( P < 0.05) and the prolonging of APTT, compared with asthmatic group. In addition, puerarin showed many physiological activities, including the improvement of blood circulation [27] and anti-inflammatory activity [37], both of which could eventually lead to the decrease in the levels of FIB.…”

Section: Discussionmentioning

confidence: 99%

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Puerarin Attenuates Ovalbumin‐Induced Lung Inflammation and Hemostatic Unbalance in Rat Asthma Model

Dong

Wang

Duan

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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Aim. We aimed to investigate and evaluate the preventive activity of puerarin on the ovalbumin-induced asthma rat model. Materials and Methods. Male Wistar rats were sensitized intraperitoneally on days 0, 7, and 14 and challenged to ovalbumin intratracheally on day 21. Groups of sensitized rats were treated randomly either with placebo, puerarin, dexamethasone, or puerarin combined with dexamethasone, from days 15 to 20. Inflammatory markers, including cell counts in bronchoalveolar lavage fluid (BALF), inflammatory cytokines, histopathology, and coagulation parameters, such as coagulation tests and the activity of coagulation factors, were analyzed. Results. Puerarin significantly inhibited the recruitment of inflammatory cells in BALF and lung tissue. At the same time, the release of IL-4, IL-10, and IFN-γ in serum and the expression of mRNAs in lung tissue homogenate were changed by puerarin. Administration of puerarin also effectively rectified the coagulation disorder in asthmatic rats, such as prothrombin time (PT) (P < 0.01), thrombin time (TT) (P < 0.05), fibrinogen (FIB) (P < 0.01),the activity of factor II (FII) (P < 0.01), the activity of factor V (FV) (P < 0.05), the activity of factor VII (FVII) (P < 0.05), the activity of factor X (FX) (P < 0.05), the activity of factor VIII (FVIII) (P < 0.01), the activity of factor IX (FIX) (P < 0.05), and the activity of factor XII (FXII) (P < 0.05). Conclusions. Our results provide a clue that puerarin was useful for the preventive of allergic airway disease in rodents.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Puerarin Attenuates Ovalbumin‐Induced Lung Inflammation and Hemostatic Unbalance in Rat Asthma Model

Dong

Wang

Duan

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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“…Several pre-clinical studies showed that puerarin inhibits inflammation and regulates the release of inflammatory mediators like TNF-α, IL-6 and IL-1β, 9,10) and it also ameliorates LPS or tetrachloride-induced kidney injury in mice by inhibiting inflammatory response. 11,12) These results suggest that puerarin exhibiting anti-inflammatory activity, may affect LPS-induced the production of pro-inflammatory cytokines, which enhance osteoclast differentiation and bone loss. In addition, puerarin has been reported to effectively inhibit bone loss in rats with estrogen deficiency or with ligature-induced periodontitis.…”

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confidence: 85%

Puerarin Prevents LPS-Induced Osteoclast Formation and Bone Loss <i>via</i> Inhibition of Akt Activation

Zhang

Yan

et al. 2016

Biological & Pharmaceutical Bulletin

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Osteolysis induced by chronic Gram-negative bacterial infection underlies many bone diseases such as osteomyelitis, septic arthritis, and periodontitis. Drugs that inhibit lipopolysaccharide (LPS)-induced osteolysis are critically needed for the prevention of bone destruction in infective bone diseases. In this study, we assessed the effect of puerarin, a natural isoflavone isolated from Pueraria lobata OHWI root, on LPS-induced osteoclastogenesis and bone loss. Our in vitro study showed that puerarin significantly inhibited LPS-induced osteoclast differentiation from osteoclast precursor RAW264.7 cells. The inhibition occurred through suppressing the production of osteoclast activating factor tumor necrosis factor (TNF)-α, interleukin (IL)-1β and prostaglandin E 2 (PGE 2 ), which led to down-regulating mRNA expression of osteoclastogenic genes including tartrate-resistant acid phosphatase (TRAP), cathepsin K and matrix metalloprotein 9 (MMP-9). Furthermore, LPS triggered activation of Akt in osteoclast precursor RAW264.7 cells, which was inhibited by puerarin treatment. In vivo, puerarin attenuated LPS-induced bone loss in a murine calvarial osteolysis model. Collectively, puerarin prevents LPS-induced osteoclast formation, function and bone loss, where the inhibition of Akt activation plays an important role. These findings provide evidences that puerarin might be beneficial as a promising candidate drug for the prevention and treatment of bacteria-induced bone destruction disease, and give new insights for understanding its possible mechanism.

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“…Puerarin (7,4′-dihydroxy-8- β -D-glucosylisoflavone, C 21 H 20 O 9 ) (Pue) [10] is a kind of flavonoids, which is extracted from Chinese herb Kudzu root and widely used in Chinese clinics as an adjuvant therapy for the treatment of angina pectoris, diabetes [11], and ischemic cerebrovascular diseases [12]. Our previous [13] and others' studies [14] have demonstrated that puerarin attenuated pressure or angiotensin II-induced cardiac hypertrophy in mice, and puerarin could also inhibit inflammation and apoptosis in LPS-stimulated cardiomyocytes [15].…”

Section: Introductionmentioning

confidence: 99%

Puerarin Protects against Cardiac Fibrosis Associated with the Inhibition of TGF-β1/Smad2-Mediated Endothelial-to-Mesenchymal Transition

Jin

Yuan

et al. 2017

PPAR Research

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Background Puerarin is a kind of flavonoids and is extracted from Chinese herb Kudzu root. Puerarin is widely used as an adjuvant therapy in Chinese clinics. But little is known about its effects on regulating cardiac fibrosis. Methods Mice were subjected to transverse aorta constriction (TAC) for 8 weeks; meanwhile puerarin was given 1 week after TAC. Cardiac fibrosis was assessed by pathological staining. The mRNA and protein changes of CD31 and vimentin in both animal and human umbilical vein endothelial cells (HUVECs) models were detected. Immunofluorescence colocalization of CD31 and vimentin and scratch test were carried out to examine TGF-β1-induced changes in HUVECs. The agonist and antagonist of peroxisome proliferator-activated receptor-γ (PPAR-γ) were used to explore the underlying mechanism. Results Puerarin mitigated TAC-induced cardiac fibrosis, accompanied with suppressed endothelial-to-mesenchymal transition (EndMT). The consistent results were achieved in HUVECs model. TGF-β1/Smad2 signaling pathway was blunted and PPAR-γ expression was upregulated in puerarin-treated mice and HUVECs. Pioglitazone could reproduce the protective effect in HUVECs, while GW9662 reversed this effect imposed by puerarin. Conclusion Puerarin protected against TAC-induced cardiac fibrosis, and this protective effect may be attributed to the upregulation of PPAR-γ and the inhibition of TGF-β1/Smad2-mediated EndMT.

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Puerarin exerts antipyretic effect on lipopolysaccharide-induced fever in rats involving inhibition of pyrogen production from macrophages

Cited by 44 publications

References 44 publications

Puerarin Attenuates Ovalbumin‐Induced Lung Inflammation and Hemostatic Unbalance in Rat Asthma Model

Puerarin Attenuates Ovalbumin‐Induced Lung Inflammation and Hemostatic Unbalance in Rat Asthma Model

Puerarin Prevents LPS-Induced Osteoclast Formation and Bone Loss <i>via</i> Inhibition of Akt Activation

Puerarin Protects against Cardiac Fibrosis Associated with the Inhibition of TGF-β1/Smad2-Mediated Endothelial-to-Mesenchymal Transition

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