Puerarin Protects Hippocampal Neurons Against Cell Death in Pilocarpine-Induced Seizures Through Antioxidant and Anti-Apoptotic Mechanisms

Xie, Ning; Wang, Cui; Lian, Yajun; Wu, Chuanjie; Zhang, Haifeng; Zhang, Qian

doi:10.1007/s10571-014-0093-2

Cited by 24 publications

(14 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, puerarin significantly prevented 1-methyl-4-phenylpyridinium (MPP + )-induced neurotoxicity in PC12 cells through inhibition of the c-Jun N-terminal kinase (JNK) signaling pathway (18). Furthermore, puerarin exerted a protective effect against epilepsy-induced brain injury through antioxidant and anti-apoptotic mechanisms (19). In line with these findings, puerarin also prevented amyloid β-induced neurotoxicity in PC12 cells.…”

Section: Discussionmentioning

confidence: 64%

“…Puerarin, known as Ge-gen in Chinese, is isolated from Radix puerariae and has protective effects on the nervous and cardiovascular system to prevent osteoporosis, liver injury and inflammation (7). An increasing body of evidence has indicated that puerarin has anti-apoptotic effects and mitigated apoptosis in multiple cell types, including neurons, microglia, osteoblasts and cardiomyocytes (8)(9)(10)(11). A previous study reported that puerarin protected PC12 cells against β-amyloid-induced cell injury, which was associated with its antioxidant effects (12).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Puerarin prevents tumor necrosis factor-α-induced apoptosis of PC12 cells via activation of the PI3K/Akt signaling pathway

Liang

Xie

2017

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

Abstract. Tumor necrosis factor-α (TNF-α), a potential proinflammatory cytokine, is an important component involved in neuronal apoptosis associated with neuroinflammation in the central nervous system. It has been reported that puerarin possesses pharmacological effects, such as anti-apoptotic, antioxidant, anti-osteoporosis, anti-inflammatory, cardioprotective and neuroprotective actions. The aim of the present study was to explore the effect of puerarin on apoptosis induced by TNF-α (3x10 5 U/l) and its detailed mechanisms in PC12 cells. MTT and flow cytometric assays were performed to evaluate cell cytotoxicity and apoptosis, respectively. An enzymatic assay was used to detect the activity of caspase-3 and caspase-9. Western blot analysis was performed to assess changes in the levels of proteins, including B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), caspase-3, Akt and phosphorylated Akt. The results showed that puerarin (25 and 50 µM) significantly suppressed TNF-α-induced apoptosis in PC12 cells. The TNF-α-induced in crease in the Bax/Bcl-2 ratio was markedly inhibited by pre-treatment with puerarin for 2 h. In addition, puerarin decreased the level of TNF-α-induced cleaved caspase-3. Furthermore, puerarin inhibited the TNF-α-induced decrease in the phosphorylation of Akt, which was abolished by LY294002, a phosphatidylinositol 3-kinase (PI3K) inhibitor, suggesting that the PI3K/Akt pathway participated in the suppressive effect of puerarin. Taken together, these findings indicated that puerarin prevented TNF-α-induced apoptosis in PC12 cells via activating of the PI3K/Akt signaling pathway, suggesting that puerarin may be a potential neuroprotective drug in the clinical treatment of neuroinflammation via anti-apoptotic mechanisms.

show abstract

Section: Discussionmentioning

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Puerarin prevents tumor necrosis factor-α-induced apoptosis of PC12 cells via activation of the PI3K/Akt signaling pathway

Liang

Xie

2017

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

show abstract

“…Treatment with E 2 , PME, or PU decreased the combined circular and random strategies of rat movement to find the hidden platform by 4%, 12%, and 18%, respectively, for the 2‐month OVX rats and by 8%, 25%, and 28%, respectively, for the 4‐month OVX rats. Thus, as noted earlier, the estrogenic activity of PU on the uterus weight or serum LH and FSH levels was not detected, and so the improvement in the impaired cognition in rats is likely to occur via other pathways, such as anti‐oxidant (Xie et al , ). The observed improvement in cognitive ability after E 2 or PU treatment, in terms of the higher latency and distance to find the platform, agreed with that previously reported in OVX mice (Xu et al , ) and Aβ‐induced cognitive impaired rats (Li et al , ).…”

Section: Discussionmentioning

confidence: 82%

Neurotherapeutic Effects of Pueraria mirifica Extract in Early- and Late-Stage Cognitive Impaired Rats

et al. 2016

View full text Add to dashboard Cite

We determined the neurotherapeutic effects of Pueraria mirifica extract (PME) and pure puerarin (PU) in comparison with 17β-estradiol (E2 ) in early- and late-stage cognitive impaired rats. Rats were ovariectomized (OVX), kept for 2 and 4 months to induce early- and late-stage cognitive impairment, respectively, and divided into four groups that were treated daily with (i) distilled water, (ii) 100 mg/kg of PME, (iii) 7 mg/kg of PU, and (iv) 80 µg/kg of E2 for 4 months. The estrogen deficiency symptoms of OVX rats were abrogated by treatment with E2 or PME, but not by treatment with PU. The mRNA level of genes associated with amyloid production (App and Bace1) and hyperphosphorylated Tau (Tau4) were upregulated together with the level of impaired cognition in the 2- and 4-month OVX rats. Treatment with E2 reduced the level of cognitive impairment more than that with PME and PU, and 2-month OVX rats were more responsive than 4-month OVX rats. All treatments down-regulated the Bace1 mRNA level in 2-month OVX rats, while PU and PME also decreased the App mRNA level in 2- and 4-month OVX rats, respectively. Only PU suppressed Tau4 expression in 2-month OVX rats. Thus, PME and PU elicit neurotherapeutic effects in different pathways, and earlier treatment is optimal. Copyright © 2016 John Wiley & Sons, Ltd.

show abstract

“…Its medicinal value is bene ted from its wide range of pharmacological properties, such as vasodilation, cardio-protection, antioxidant, anti-apoptotic and reducing insulin resistance [15]. Puerarin plays a protective role against cell damage caused by pathological factors [16]. Puerarin has been reported to reduce Caspase-3 expression by activating PI3K/AKT signaling pathway, and signi cantly improve tumor necrosis factor-α (TNF-α) induced apoptosis in PC12 cells [17].…”

Section: Introductionmentioning

confidence: 99%

Puerarin Inhibits FUNDC1-mediated Mitochondrial Autophagy and CSE-induced Apoptosis of Human Bronchial Epithelial Cells by Activating the PI3K/AKT/MTOR Signaling Pathway

Wang¹,

Jiang²,

Wang³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Growing evidence suggests that the pathogenesis of chronic obstructive pulmonary disease (COPD) is related to mitochondrial autophagy mediated by FUN14 domain protein 1 (FUNDC1). Recent studies have shown that puerarin has protective effects against excessive oxidative damage in cells. We hypothesized that puerarin might be involved in the progression of COPD induced by cigarette smoke extract (CSE) by regulating FUNDC1-mediated mitochondrial autophagy.Material/Methods: Different concentrations of puerarin were used to intervene in CSE-treated human bronchial epithelial cells (HBECs). MTT assay and flow cytometry were used to detect cell activity and apoptosis. The mitochondrial membrane potential (MMP) level, reactive oxygen species (ROS) content and ATP content were detected by flow cytometry or kits; Western Blotting was used to detect mitochondrial autophagy-related proteins expression such as DRP1, FUNDC1, PINK1, Parkin. Next, protein phosphatase inhibitor (PH0321) was used to inhibit the phosphorylation of FUNDC1, the changes of MMP, ROS, ATP and mitochondrial autophagy related proteins were detected. Furthermore, mitochondrial autophagy inhibitors (Mdivi) were used to block autophagy, and then the changes of mitochondrial autophagy, cell activity and apoptosis were detected, respectively. Finally, the changes of PI3K/AKT/mTOR signaling pathway-related proteins were detected by Western Blotting.Results: Puerarin reversed cell activity decrease and apoptosis increase of CSE-induced HBECs, and down-regulated apoptosis-related proteins expression. Puerarin up-regulated MMP level and ATP content in CSE-induced HBECs, and down-regulated ROS content and mitochondrial autophagy-related proteins expression. PH0321 promotes mitochondrial autophagy by promoting the dephosphorylation of FUNDC1, puerarin inhibits the dephosphorylation of FUNDC1, up-regulated the MMP level and ATP content of CSE-induced HBECs, reduces ROS content and mitochondrial autophagy-related proteins expression. Compared with Mdivi group, the protein expression changes of PINK1 and Parkin in puerarin-treated group were not significantly different, and the cell activity decrease and apoptosis increase of CSE-induced HBECs were also significantly reversed by puerarin, which was not significantly different from Mdivi group. Puerarin significantly upregulated the protein expression of p-PI3K, p-AKT and p-mTOR, suggesting that puerarin may participate in the progression of COPD by activating the PI3K/AKT/mTOR signaling pathway.Conclusions: Puerarin inhibits FUNDC1-mediated mitochondrial autophagy and CSE-induced apoptosis of HBECs by activating the PI3K/AKT/mTOR signaling pathway.

show abstract

Puerarin Protects Hippocampal Neurons Against Cell Death in Pilocarpine-Induced Seizures Through Antioxidant and Anti-Apoptotic Mechanisms

Cited by 24 publications

References 19 publications

Puerarin prevents tumor necrosis factor-α-induced apoptosis of PC12 cells via activation of the PI3K/Akt signaling pathway

Puerarin prevents tumor necrosis factor-α-induced apoptosis of PC12 cells via activation of the PI3K/Akt signaling pathway

Neurotherapeutic Effects of Pueraria mirifica Extract in Early- and Late-Stage Cognitive Impaired Rats

Puerarin Inhibits FUNDC1-mediated Mitochondrial Autophagy and CSE-induced Apoptosis of Human Bronchial Epithelial Cells by Activating the PI3K/AKT/MTOR Signaling Pathway

Contact Info

Product

Resources

About