Puerarin protects PC12 cells against β‐amyloid‐induced cell injury

Zhang, Haiying; Liu, Yiheng; Wang, Hong Quan; Xu, Jin; Hu, Haitao

doi:10.1016/j.cellbi.2008.07.006

Cited by 78 publications

(44 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…32 Additionally, Puerarin from P. thunbergiana has been found to exert a neuroprotective effect against beta-amyloid-induced cell death in PC-12 cells. 33 Taken together, these findings and the results of the present study indicate that other unidentified constituents of the herbs in the PD-1 prescription may have participated synergistically in the neuroprotective action observed in the present study. Accordingly, future studies should be conducted to elucidate the neuroprotective mechanisms of PD-1.…”

Section: Discussionsupporting

confidence: 57%

Preventive role of PD‐1 on MPTP‐induced dopamine depletion in mice

Jung

Son

Jin

et al. 2010

Cell Biochemistry & Function

View full text Add to dashboard Cite

Many current studies of Parkinson's disease (PD) suggest that inflammation is involved in the neurodegenerative process. PD-1, a traditional Korean medicine, used to treat various brain diseases in Korea. This study was designed to investigate the effect of PD-1 extract in the Parkinson's model of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) lesioned mice. The MPTP administration caused the dopamine neuron loss in the striatum and substantia nigra pars compacta (SNpc), which was demonstrated by a depletion of tyrosine hydroxylase (TH). In addition, a reduction of bcl-2 expression with elevation of bax expression, caspase-3 activation, and release of cytochrome c into cytosol in dopaminergic neurons of SNpc were noted. Oral administration of PD-1 extract (50 and 100 mg kg(-1)) attenuated the MPTP-induced depletion of TH proteins in the striatum and SNpc and prevented the apoptotic effects. These results indicate that PD-1 extract is able to protect dopaminergic neurons from MPTP-induced neuronal death, with important implications for the treatment of PD.

show abstract

Section: Discussionsupporting

confidence: 57%

Preventive role of PD‐1 on MPTP‐induced dopamine depletion in mice

Jung

Son

Jin

et al. 2010

Cell Biochemistry & Function

View full text Add to dashboard Cite

show abstract

“…An increasing body of evidence has indicated that puerarin has anti-apoptotic effects and mitigated apoptosis in multiple cell types, including neurons, microglia, osteoblasts and cardiomyocytes (8)(9)(10)(11). A previous study reported that puerarin protected PC12 cells against β-amyloid-induced cell injury, which was associated with its antioxidant effects (12). Another study confirmed that puerarin protected differentiated PC12 cells from H 2 O 2 -induced apoptosis via Akt phosphorylation (13).…”

Section: Introductionsupporting

confidence: 53%

Puerarin prevents tumor necrosis factor-α-induced apoptosis of PC12 cells via activation of the PI3K/Akt signaling pathway

Liang

Xie

2017

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

Abstract. Tumor necrosis factor-α (TNF-α), a potential proinflammatory cytokine, is an important component involved in neuronal apoptosis associated with neuroinflammation in the central nervous system. It has been reported that puerarin possesses pharmacological effects, such as anti-apoptotic, antioxidant, anti-osteoporosis, anti-inflammatory, cardioprotective and neuroprotective actions. The aim of the present study was to explore the effect of puerarin on apoptosis induced by TNF-α (3x10 5 U/l) and its detailed mechanisms in PC12 cells. MTT and flow cytometric assays were performed to evaluate cell cytotoxicity and apoptosis, respectively. An enzymatic assay was used to detect the activity of caspase-3 and caspase-9. Western blot analysis was performed to assess changes in the levels of proteins, including B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), caspase-3, Akt and phosphorylated Akt. The results showed that puerarin (25 and 50 µM) significantly suppressed TNF-α-induced apoptosis in PC12 cells. The TNF-α-induced in crease in the Bax/Bcl-2 ratio was markedly inhibited by pre-treatment with puerarin for 2 h. In addition, puerarin decreased the level of TNF-α-induced cleaved caspase-3. Furthermore, puerarin inhibited the TNF-α-induced decrease in the phosphorylation of Akt, which was abolished by LY294002, a phosphatidylinositol 3-kinase (PI3K) inhibitor, suggesting that the PI3K/Akt pathway participated in the suppressive effect of puerarin. Taken together, these findings indicated that puerarin prevented TNF-α-induced apoptosis in PC12 cells via activating of the PI3K/Akt signaling pathway, suggesting that puerarin may be a potential neuroprotective drug in the clinical treatment of neuroinflammation via anti-apoptotic mechanisms.

show abstract

“…Another study reported that puerarin exhibited a protective effect against the lipopolysaccharide (LPS)-induced apoptosis of H9c2 cardiomyocytes by reversing LPS-induced downregulation of Bax and upregulation of Bcl-2 (15). In addition, puerarin afforded protection against beta-amyloid-induced neurotoxicity by inhibiting apoptosis in PC12 cells, which contributed to activation of the PI3K-dependent signaling pathway in association with Akt phosphorylation (16). Although anti-apoptotic mechanisms have been demonstrated to be involved in the protective effect of puerarin in the nervous and cardiovascular system (17,18), in the present study, puerarin demonstrated antitumor effects by reducing cell viability and inducing cell apoptosis in SW1353 cells, which may be important for the clinical treatment of patients with cancer.…”

Section: Discussionmentioning

confidence: 99%

Puerarin induces cell apoptosis in human chondrosarcoma cell line SW1353 via inhibition of the PI3K/Akt signaling pathway

Huang

Cao

et al. 2017

Oncol Lett

View full text Add to dashboard Cite

Abstract. Chondrosarcoma is a malignant soft tissue sarcoma with poor prognosis. Puerarin has been demonstrated to possess anticancer properties; however, the effects of puerarin in human chondrosarcoma cells remain unknown. The present study aimed to investigate the anticancer effects of puerarin in SW1353 human chondrosarcoma cells. SW1353 cells were treated with increasing concentrations of puerarin for different durations. Cell viability was evaluated using MTT assays. Cell apoptosis rates were determined by flow cytometry. The activities of caspase-3 and caspase-9 were measured by enzymatic assay. The expression of RAC-alpha serine/threonine-protein kinase (Akt), phosphorylated-Akt, caspase-3 and apoptosis-associated proteins, including B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax) were detected by western blotting. Puerarin significantly decreased cell viability and significantly induced apoptosis of SW1353 cells. In addition, puerarin significantly increased the enzymatic activities of caspase-3 and caspase-9. Puerarin treatment suppressed the expression of p-Akt and Bcl-2 but promoted the expression of Bax and cleaved caspase-3 in SW1353 cells. Notably, the phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 abrogated the decreased phosphorylation of Akt, suggesting that the PI3K/Akt signaling pathway is involved in mediating the anticancer effects of puerarin. The data from the present study indicated that puerarin exhibits anticancer effects in SW1353 cells and may be a potential therapeutic drug for patients with chondrosarcoma.

show abstract

Puerarin protects PC12 cells against β‐amyloid‐induced cell injury

Cited by 78 publications

References 38 publications

Preventive role of PD‐1 on MPTP‐induced dopamine depletion in mice

Preventive role of PD‐1 on MPTP‐induced dopamine depletion in mice

Puerarin prevents tumor necrosis factor-α-induced apoptosis of PC12 cells via activation of the PI3K/Akt signaling pathway

Puerarin induces cell apoptosis in human chondrosarcoma cell line SW1353 via inhibition of the PI3K/Akt signaling pathway

Contact Info

Product

Resources

About