Puerarin suppresses inflammation and ECM degradation through Nrf2/HO-1 axis in chondrocytes and alleviates pain symptom in osteoarthritic mice

Chen, Ximiao; Huang, Chongan; Sun, Haiqiu; Hong, Haofeng; Jin, Jie; Bei, Chaoyong; Lu, Zhongqiu; Zhang, Xiaolei

doi:10.1039/d0fo03076g

Cited by 35 publications

(24 citation statements)

References 36 publications

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“…explored that puerarin suppresses inflammation and ECM degradation through Nrf2/HO‐1 pathway in the osteoarthritic mice model. [ 37 ] In another study, Chang et al. verified that puerarin can attenuate (Lipopolysaccharide) LPS‐induced inflammation in human umbilical vein endothelial cells (HUVECs) improving mitochondrial respiratory function.…”

Section: Resultsmentioning

confidence: 99%

Regulated Exogenous/Endogenous Inflammation via “Inner‐Outer” Medicated Electrospun Fibers for Promoting Tissue Reconstruction

Zhou

Saiding

Wang

et al. 2022

Adv Healthcare Materials

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Regenerative medicine aims to provide solutions for structural and functional recovery in conditions where organs suffer from varying degrees of diseases or injuries. However, the exogenous inflammation triggered by implanted biomaterials and endogenous inflammation caused by some disease or tissue destruction has not been solved properly yet. Herein, a functional "inner-outer" medicated core-shell electrospun fibrous membrane is fabricated with RGD surface modification for exogenous inflammation suppression and puerarin loading in the core for long-term endogenous inflammation inhibition through microsol electrospinning technique. The "outer" RGD significantly increases biocompatibility of fibrous membrane through promoting cell viability, adhesion, and proliferation while the "inner" puerarin suppresses inflammatory gene expression via sustained drug release in vitro. Moreover, in a rat abdominal wall hernia model, the functional fibrous membrane successfully reduces exogenous and endogenous inflammation response and promotes wound healing through collagen deposition, smooth muscle formation, and vascularization. In summary, the functional "inner-outer" medicated fibrous membrane holds a great potential for clinical treatment of diseases that needs tissue reconstruction structurally and functionally accompanied by immunoregulation.

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Section: Resultsmentioning

confidence: 99%

Regulated Exogenous/Endogenous Inflammation via “Inner‐Outer” Medicated Electrospun Fibers for Promoting Tissue Reconstruction

Zhou

Saiding

Wang

et al. 2022

Adv Healthcare Materials

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“…OA has become a global challenges for the elderly. The main pathological features are cartilage matrix degradation and synovium inflammation ( Chen et al, 2021 ). While much has been learned recently regarding the pathogenesis of OA, the treatment outcome is also unsatisfactory due to the unclear pathogenesis.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-10a-3p Improves Cartilage Degeneration by Regulating CH25H-CYP7B1-RORα Mediated Cholesterol Metabolism in Knee Osteoarthritis Rats

Zhang

Shi

et al. 2021

Front. Pharmacol.

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Osteoarthritis (OA) is a worldwide degenerative joint disease that seriously impaired the quality of life of patients. OA has been established as a disease with metabolic disorder. Cholesterol 25-hydroxylase (CH25H) was proved to play a key role in cartilage cholesterol metabolism. However, the biological function and mechanism of CH25H in OA remains further investigation. Growing researches have proved the vital roles of miRNAs in OA progression. In this study, we screened out miR-10a-3p through high-throughput miRNA sequencing which may bind to CH25H. Molecular mechanism investigation indicated that miR-10a-3p is an upstream target of CH25H. Functional exploration revealed miR-10a-3p suppressed the inflammatory responses, cholesterol metabolism and extracellular matrix (ECM) degradation in primary chondrocytes. Moreover, rescue assays implied that miR-10a-3p reversed CH25H plasmids induced inflammatory cytokine production and ECM degradation. Furthermore, the OA rat model was established to explore the function of miR-10a-3p in vivo. The results showed that miR-10a-3p can recover the OA features through targeting CH25H/CYP7B1/RORα axis. In conclusion, these findings implied a crucial role of miR-10a-3p/CH25H/CYP7B1/RORα axis in OA, which may provide a promising therapeutic strategy for OA.

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“…Previous studies reported that inflammatory cytokines like members of the tumor necrosis factor (TNF)-superfamily could change the tumor microenvironment and promote the progression of colorectal cancer through activation of EMT. These pro-inflammatory mediators are modulated via the transcription factor NF-kB (113)(114)(115)(116). It has also been reported that focal adhesion kinase (FAK) associated with NF-kB regulates the cancer cells capacity of survival, invasiveness, and metastasis (117)(118)(119)(120).…”

Section: Colorectal Cancermentioning

confidence: 99%

Resveratrol and Its Analogs: Potent Agents to Reverse Epithelial-to-Mesenchymal Transition in Tumors

et al. 2021

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Epithelial-to-mesenchymal transition (EMT), a complicated program through which polarized epithelial cells acquire motile mesothelial traits, is regulated by tumor microenvironment. EMT is involved in tumor progression, invasion and metastasis via reconstructing the cytoskeleton and degrading the tumor basement membrane. Accumulating evidence shows that resveratrol, as a non-flavonoid polyphenol, can reverse EMT and inhibit invasion and migration of human tumors via diverse mechanisms and signaling pathways. In the present review, we will summarize the detailed mechanisms and pathways by which resveratrol and its analogs (e.g. Triacetyl resveratrol, 3,5,4’-Trimethoxystilbene) might regulate the EMT process in cancer cells to better understand their potential as novel anti-tumor agents. Resveratrol can also reverse chemoresistance via EMT inhibition and improvement of the antiproliferative effects of conventional treatments. Therefore, resveratrol and its analogs have the potential to become novel adjunctive agents to inhibit cancer metastasis, which might be partly related to their blocking of the EMT process.

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Puerarin suppresses inflammation and ECM degradation through Nrf2/HO-1 axis in chondrocytes and alleviates pain symptom in osteoarthritic mice

Abstract: Puerarin suppresses the NF-κB signaling pathway by activating the Nrf2/HO-1 axis, thereby inhibiting IL-1β-induced inflammation and ECM degradation in Osteoarthritis.

Cited by 35 publications

References 36 publications

Regulated Exogenous/Endogenous Inflammation via “Inner‐Outer” Medicated Electrospun Fibers for Promoting Tissue Reconstruction

Regulated Exogenous/Endogenous Inflammation via “Inner‐Outer” Medicated Electrospun Fibers for Promoting Tissue Reconstruction

MicroRNA-10a-3p Improves Cartilage Degeneration by Regulating CH25H-CYP7B1-RORα Mediated Cholesterol Metabolism in Knee Osteoarthritis Rats

Resveratrol and Its Analogs: Potent Agents to Reverse Epithelial-to-Mesenchymal Transition in Tumors

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