PUFAs supplementation affects the renal expression of pannexin 1 and connexins in diabetic kidney of rats

Blood Omega-3 Fatty Acids Are Inversely Associated With Albumin-Creatinine Ratio in Young and Healthy Adults (The Omega-Kid Study)

“…Priante et al (24) provided a number of mechanism how n-3 PUFAs counteract renal inflammation and fibrosis. Further, n-3 PUFA supplementation affects cell junctions and protects from distal tubular cell damage in rat kidneys, thus directly and indirectly affecting albuminuria (25,26). There is increasing evidence for differing individual effects of EPA and DHA on cardiometabolic risk factors (27).…”

Sex-specific effects of metformin and liraglutide on renal pathology and expression of connexin 45 and pannexin 1 following long-term high-fat high-sugar diet

“…So far, the expression of Cx45 has been found in renal vascular smooth muscle cells of afferent and efferent arterioles, intraglomerular and extraglomerular mesangial cells, renin producing juxtaglomerular cells, podocytes and distal tubules (Hanner et al, 2008(Hanner et al, , 2010. Nevertheless, in our previous work (Saric et al, 2017;Luetić et al, 2020), we found that Cx45 is the most prominent Cx expressed in proximal tubules of the rat kidney and its expression is substantially decreased in streptozotocin-induced diabetes mellitus (DM) type 1. However, there are still insufficient data about the exact role of Cx45 and the findings of its distribution in the kidneys are conflicting (Hanner et al, 2010).…”

In focus in HCB

“…Recently, Vitlov Uljevic et al (2019) demonstrated that dietary supplements with n-3 form of PUFAs resulted in an alleviation of renal distal tubular cell damage in a rat experimental model of diabetes mellitus. This same group has now extended this study by investigating a possible positive effect of dietary supplementation with differing n-6/n-3 PUFA ratios on diabetic nephropathy through an alteration in connexin and pannexin protein expression (Luetic et al 2020). The study involved staining tissues from groups of diabetic rats fed a diet incorporating varying ratios of n-6/n-3 PUFA for immunofluorescence microscopy with several different anti-connexin and -pannexin antibodies, followed by semi-quantitative image analysis, focusing on the renal cortex.…”