Pulling-Force Spinning Top for Serum Separation Combined with Paper-Based Microfluidic Devices in COVID-19 ELISA Diagnosis

Gong, Fanwu; Wei, Hua-Xing; Qi, Ji; Ma, Huan; Liu, Lianxin; Weng, Jianping; Zheng, Xucai; Li, Qiangsheng; Zhao, Dan; Fang, Haopeng; Liu, Liu; He, Hongliang; Ma, Cuichen; Han, Jinglong; Sun, Anyuan; Wang, Baolong; Jin, Tengchuan; Li, Bowei; Li, Bofeng

doi:10.1021/acssensors.1c00773

Cited by 54 publications

(48 citation statements)

References 52 publications

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“…Proteins, like enzymes and antigens, were also detected by μPADs. For example, Gong’s team [ 116 ] developed a microfluidic platform that collected human serum by a pulling-force spinning top (PFST) and paper-based microfluidic enzyme-linked immunosorbent assay (ELISA) for quantity of IgA/IgM/IgG in an instrument-free way. It can easily isolate the serum without any clinical apparatus, and a portable smart phone made it easy to record the intensity signal.…”

Section: Optical Detection Methodsmentioning

confidence: 99%

Recent Advances and Applications in Paper-Based Devices for Point-of-Care Testing

Hou

Guo

et al. 2022

J. Anal. Test.

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Point-of-care testing (POCT), as a portable and user-friendly technology, can obtain accurate test results immediately at the sampling point. Nowadays, microfluidic paper-based analysis devices (μPads) have attracted the eye of the public and accelerated the development of POCT. A variety of detection methods are combined with μPads to realize precise, rapid and sensitive POCT. This article mainly introduced the development of electrochemistry and optical detection methods on μPads for POCT and their applications on disease analysis, environmental monitoring and food control in the past 5 years. Finally, the challenges and future development prospects of μPads for POCT were discussed.

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Section: Optical Detection Methodsmentioning

confidence: 99%

Recent Advances and Applications in Paper-Based Devices for Point-of-Care Testing

Hou

Guo

et al. 2022

J. Anal. Test.

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“…To eliminate centrifugation steps in sample preparation, plasma separation membranes were mostly employed in lateral flow assays or 3D μ PADs. [ 79 , 80 , 81 , 82 ] Based on size exclusion, blood cells are trapped in the membrane while the plasma is separated and transmitted into the detection zones for detection. In comparison to microfluidic chips, those fully integrated paper‐based devices have the advantages of rapid reaction times, quick readout, ease of use, and low cost.…”

Section: Smartphone‐based Point‐of‐care Testing For Virus Detectionmentioning

confidence: 99%

Virus Detection: From State‐of‐the‐Art Laboratories to Smartphone‐Based Point‐of‐Care Testing

et al. 2022

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Infectious virus outbreaks pose a significant challenge to public healthcare systems. Early and accurate virus diagnosis is critical to prevent the spread of the virus, especially when no specific vaccine or effective medicine is available. In clinics, the most commonly used viral detection methods are molecular techniques that involve the measurement of nucleic acids or proteins biomarkers. However, most clinic‐based methods require complex infrastructure and expensive equipment, which are not suitable for low‐resource settings. Over the past years, smartphone‐based point‐of‐care testing (POCT) has rapidly emerged as a potential alternative to laboratory‐based clinical diagnosis. This review summarizes the latest development of virus detection. First, laboratory‐based and POCT‐based viral diagnostic techniques are compared, both of which rely on immunosensing and nucleic acid detection. Then, various smartphone‐based POCT diagnostic techniques, including optical biosensors, electrochemical biosensors, and other types of biosensors are discussed. Moreover, this review covers the development of smartphone‐based POCT diagnostics for various viruses including COVID‐19, Ebola, influenza, Zika, HIV, et al. Finally, the prospects and challenges of smartphone‐based POCT diagnostics are discussed. It is believed that this review will aid researchers better understand the current challenges and prospects for achieving the ultimate goal of containing disease‐causing viruses worldwide.

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“…The automated enzyme-linked immunosorbent assay (ELISA) on-chip microfluidic platform developed by Gonzalez-Gonzalez et al, the Opto microfluidic sensing platform based on localizes surface plasmon resonance (LSPR) developed by Funari et al, paper-based pulling force spinning top microfluidic devices designed by Gong et al, microfluidic chemiluminescent ELISA technique by Tan et al, and the reciprocating flowing immunobinding strategy by Liu et al detect anti-SARS-CoV-2 antibodies by spectrometer-based detection techniques ( Table 1 ) [ 26 , 27 , 28 , 29 , 30 ].…”

Section: Microfluidics In Sars-cov-2mentioning

confidence: 99%

“…In contrast, other spectrometer-based detection techniques stated above can detect anti-S or anti-N IgG. This technique has another advantage in that it can directly collect serum from blood samples by pulling force spinning top technique within 4–5 min and detect the IgA, IgM, and IgG with high sensitivity (i.e., 97.1% for IgA, 91.4% for IgM, and 85.7% for IgG) and specificity (i.e., 100% for IgA, 92.8% for IgM, and 100% for IgG) by measuring various combinations of these antibodies as described by Gong et al [ 27 ]. The other spectrometer-based detection techniques rely on either serum or plasma samples.…”

Section: Microfluidics In Sars-cov-2mentioning

confidence: 99%

Microfluidics Technology in SARS-CoV-2 Diagnosis and Beyond: A Systematic Review

Jamiruddin

Meghla

Islam

et al. 2022

Life

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With the progression of the COVID-19 pandemic, new technologies are being implemented for more rapid, scalable, and sensitive diagnostics. The implementation of microfluidic techniques and their amalgamation with different detection techniques has led to innovative diagnostics kits to detect SARS-CoV-2 antibodies, antigens, and nucleic acids. In this review, we explore the different microfluidic-based diagnostics kits and how their amalgamation with the various detection techniques has spearheaded their availability throughout the world. Three other online databases, PubMed, ScienceDirect, and Google Scholar, were referred for articles. One thousand one hundred sixty-four articles were determined with the search algorithm of microfluidics followed by diagnostics and SARS-CoV-2. We found that most of the materials used to produce microfluidics devices were the polymer materials such as PDMS, PMMA, and others. Centrifugal force is the most commonly used fluid manipulation technique, followed by electrochemical pumping, capillary action, and isotachophoresis. The implementation of the detection technique varied. In the case of antibody detection, spectrometer-based detection was most common, followed by fluorescence-based as well as colorimetry-based. In contrast, antigen detection implemented electrochemical-based detection followed by fluorescence-based detection, and spectrometer-based detection were most common. Finally, nucleic acid detection exclusively implements fluorescence-based detection with a few colorimetry-based detections. It has been further observed that the sensitivity and specificity of most devices varied with implementing the detection-based technique alongside the fluid manipulation technique. Most microfluidics devices are simple and incorporate the detection-based system within the device. This simplifies the deployment of such devices in a wide range of environments. They can play a significant role in increasing the rate of infection detection and facilitating better health services.

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Pulling-Force Spinning Top for Serum Separation Combined with Paper-Based Microfluidic Devices in COVID-19 ELISA Diagnosis

Cited by 54 publications

References 52 publications

Recent Advances and Applications in Paper-Based Devices for Point-of-Care Testing

Recent Advances and Applications in Paper-Based Devices for Point-of-Care Testing

Virus Detection: From State‐of‐the‐Art Laboratories to Smartphone‐Based Point‐of‐Care Testing

Microfluidics Technology in SARS-CoV-2 Diagnosis and Beyond: A Systematic Review

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