Pulmonary hydatidosis genotypes isolates from human clinical surgery based on sequencing of mitochondrial genes in Fars, Iran

Mardani, Parviz; Ezabadi, Ali Talebi; Sedaghat, Bahareh; Sadjjadi, Seyed Mahmoud

doi:10.1186/s13019-021-01547-2

Cited by 9 publications

(9 citation statements)

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“…A total of 24 human sera from different counties of Fars province, southern Iran were collected and stored at – 20 °C until use. In this region E. granulosus sensu stricto followed by E. canadensis (G6/7) are the most frequent species/genotypes in lungs CE [ 5 ]. The pulmonary cyst in the patients were classified according to the location of the cysts and their size.…”

Section: Methodsmentioning

confidence: 99%

“…Cystic echinococcosis(CE)/hydatidosis is an important zoonotic parasitic disease caused by the larval stage or metacestode of the tapeworm Echinococcus granulosus [ 1 , 2 ]. The disease is still a major health problem all over the world including Iran [ 3 – 5 ]. Human cases of the disease are routinely reported in different hospitals of country [ 4 – 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…Different species/genotypes of Echinococcus including E. granulosus sensu stricto (s.s) (G1–G3), E. equinus (G4), E. ortleppi (G5), E. canadensis (G6–G10), E. multilocularis , E. vogeli , E. oligarthrus , E. felidis , and E. shiquicus has been reported in the world [ 10 – 12 ]. A number of above species and genotypes including E. granulosus s.s, E. canadensis, E. ortleppi have been reported from different organs of humans and animals in Iran [ 5 , 13 – 17 ]. Diagnosis and treatment of lungs CE is difficult due to confusion with other lung diseases and the fact that infection may remain asymptomatic or for a long time and then manifest itself as a severe and debilitating condition [ 9 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Proteomics investigation of human sera for determination of postoperative indicators of pulmonary cystic echinococcosis

Sadjjadi

Hajjaran

Sedaghat

et al. 2023

J Cardiothorac Surg

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Background Cystic echinococcosis (CE)/hydatidosis is an important zoonotic parasitic disease caused by the larval stage of Echinococcus granulosus. The disease is a major health problem all over the world. Finding specific and sensitive biomarkers for follow-up of CE in patients after surgery is essential. Using proteomics methods, the present study aimed to evaluate post-surgical treatment by finding probable biomarker/s in the serum of human lungs CE. Methods A total of 24 human sera were tested. These sera included eight confirmed lung/s CE patients sera before surgery (BS), eight sera 12 months post-surgery (12MPS) as well as eight control sera from healthy people. Proteomics methods including 2DE and LC–MS/MS were performed on the specimens followed by bioinformatics analysis. Differentially expressed proteins (DEP) were detected and, separately integrated with protein–protein interaction (PPI) data to construct the PPI network. Results A total of 171 protein spots were detected in three groups including BS, 12MPS, and control groups; of which a total of 106 DEP have been expressed based on fold changes > = 2 and p-value < 0.05. More analysis was performed and a total of 10 protein spots were selected for identification by mass spectrometry showing the following proteins: APOA1, BGN, SPP2, EAF1, ACOXL, MRPL55, MCTP2, SEPTIN1, B4GALNT1, and ZNF843. Based on centrality parameters of the PPI network (degree and betweenness) five Hub-bottlenecks proteins with significant centrality values were found including APOA1, BGN, SPP2, EAF1, and ACOXL. Conclusion This study showed five proteins as hub-bottleneck proteins; of which APOA1 was more prominent. It can be concluded that a change in expression of this protein in patients’ sera could be used as an indicator tool for the achievement of lungs CE surgical therapy.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Proteomics investigation of human sera for determination of postoperative indicators of pulmonary cystic echinococcosis

Sadjjadi

Hajjaran

Sedaghat

et al. 2023

J Cardiothorac Surg

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“…On the other hand, it is considered that an antigen with the specific genotype is likely developed different immune responses in compare with the others. The most prevalent genotypes of human CE in Iran has been reported to be E. granulosus s.s. followed by E. canadensis (22)(23)(24)(25)(26). Application of antigen B originated from E. granulosus s.s. (E. g ss-AgB) or E. canadensis (E.c-AgB), alone could be questionable.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Ability of Antigen B Originated from Echinococcus granulosus sensu stricto and E. canadensis For the Diagnosis of Confirmed Human Cystic Echinococcosis Using ELISA

Sadjjadi¹,

Mohammadzadeh²,

Hafezi³

et al. 2022

IJPA

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Background: Cystic echinococcosis (CE) is an important zoonotic parasitic disease worldwide. Application of species-specific antigen for serodiagnosis of human CE has not been utilized, so far. In this regard, AgB originated from Echinococcus granulosus sensu stricto (G1-G3) and E. canadensis (G6/G7) CE cysts, confirmed by molecular biology and sequencing was used for evaluation of their ability in the diagnosis of confirmed human CE. Methods: The hydatid cyst fluid (HCF) of E. granulosus sensu stricto and E. canadensis species were separately, used for preparation of AgB during 2017-2018 in Shiraz and Tehran, Iran. A total of 45 sera samples from confirmed CE patients, 102 sera from healthy people as negative control and 44 sera from other parasitic diseases, were used for measurement of the diagnostic ability of antigen B originated from E. granulosus sensu stricto and E. canadensis species of CE, alone or in 50%:50% mixture using ELISA method. Results: Overall, 38 (84.4%) out of 45 confirmed human CE were positive by ELISA using AgB originated from E. granulosus sensu stricto. This items for AgB originated from E. canadensis was 39 (86.6) out of 45 serum samples. A total of 39 out of 45 samples (86.6%) showed positivity by a mixture of antigen B originating from both species. The specificity of the above tests was calculated as 93.15%, 96.58%, and 93.84%, respectively. Conclusion: Due to the diversity of the cyst species in human population, application of AgB from prevalent species alone or in combination with other species is suggested

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“…WHO included cystic echinococcosis in a subgroup of selected neglected tropic diseases (NTDs) to be addressed within its 2008-2015 strategic plans to control NTDs. 2 In the parasite’s life cycle, the primary host is generally a canine which releases ova in its feces, the intermediate hosts (sheep, cattle, horses, goats, pigs) then ingest the infected grass or vegetables or drink infected water and become infested? Embryos are released in the intestine of intermediate host and penetrate the duodenal or jejunal wall from where they enter the portal circulation.…”

Section: Introductionmentioning

confidence: 99%

Isolated pericardial cystic Echinococcosis: A rare clinical presentation

Thapaliya¹,

Ali²,

Bhutta

2022

Pak J Med Sci

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Isolated pericardial Hydatid cyst without involvement of other viscera is a rare condition with reported incidence of 0.5-2% of all cases of cystic echinococcosis even in the countries endemic for the disease. Hydatid disease is a major public health concern in the animal raising regions worldwide. Pericardial hydatid disease can be asymptomatic or present with varying symptoms from atypical chest pain, arrhythmias, rupture and tamponade to anaphylaxis. Early diagnosis and surgical treatment is necessary to prevent fatal complications. Here we report a case of symptomatic isolated pericardial hydatid cyst who presented with epigastric pain. doi: https://doi.org/10.12669/pjms.38.3.4965 How to cite this:Thapaliya P, Ali TA, Bhutta MM. Isolated pericardial cystic Echinococcosis: A rare clinical presentation. Pak J Med Sci. 2022;38(3):---------. doi: https://doi.org/10.12669/pjms.38.3.4965 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Pulmonary hydatidosis genotypes isolates from human clinical surgery based on sequencing of mitochondrial genes in Fars, Iran

Cited by 9 publications

References 43 publications

Proteomics investigation of human sera for determination of postoperative indicators of pulmonary cystic echinococcosis

Proteomics investigation of human sera for determination of postoperative indicators of pulmonary cystic echinococcosis

Evaluation of the Ability of Antigen B Originated from Echinococcus granulosus sensu stricto and E. canadensis For the Diagnosis of Confirmed Human Cystic Echinococcosis Using ELISA

Isolated pericardial cystic Echinococcosis: A rare clinical presentation

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