Pulmonary imaging with a scanning acoustic microscope discriminates speed-of-sound and shows structural characteristics of disease

Miura, Katsutoshi; Yamamoto, Seiji

doi:10.1038/labinvest.2012.135

Cited by 29 publications

(27 citation statements)

References 11 publications

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“…From previous observations, carcinomas had slow SOS if few desmoplastic reactions occurred. 4,5 Papillary carcinomas showed intermediate SOS. Although epithelial carcinoma cells themselves displayed slow SOS, fibrous cores displayed great SOS, and the average SOS approached intermediate levels.…”

Section: Discussionmentioning

confidence: 93%

“…3 SAM uses US at 120 MHz with almost the same resolution (approximately 12.5 µm) as the low magnification of a light microscope (LM). The authors recently reported that SAM could be used to generate useful information on the lung, 4 stomach, 5 and lymph node 6 lesions. To date, no reports have been found on the application of SAM in thyroid histology.…”

Section: Introductionmentioning

confidence: 99%

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Histological evaluation of thyroid lesions using a scanning acoustic microscope

Miura¹,

Mineta²

2014

PLMI

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Video abstractPoint your SmartPhone at the code above. If you have a QR code reader the video abstract will appear. Or use: http://dvpr.es/1et29bE Dovepress submit your manuscript | www.dovepress.com Dovepress 1 O r I g I n a L r e s e a r c h open access to scientific and medical research Open access Full Text article http://dx.Purpose: A scanning acoustic microscope (SAM) uses an ultrasound to image an object by plotting the speed-of-sound (SOS) through tissues on screen. Because hard tissues result in great SOS, SAM can provide data on the tissue elasticity. This paper investigated the utility of SAM in evaluating thyroid lesions. Methods: Formalin-fixed, paraffin sections were scanned with a 120 MHz transducer. SOS through each area was calculated and plotted on the screen to provide histological images, and SOS of each lesion was compared and statistically analyzed. Results: High-concentrated colloids, red blood cells, and collagen fibers showed great SOS, while low-concentrated colloids, parathyroids, lymph follicles, and epithelial tissues including carcinomas demonstrated lower SOS. SAM clearly discriminated structure of thyroid components corresponding to low magnification of light microscopy. Thyroid tumors were classified into three groups by average SOS: the fast group consisted of follicular adenomas/carcinomas and malignant lymphomas; the slow group contained poorly differentiated/undifferentiated carcinomas; and the intermediate group comprised papillary/medullary carcinomas. Fragmented colloids, irregular-shaped follicles, and desmoplastic reactions were observed in the invasive area of surrounding carcinomas. Conclusion: The SAM imaging method had the following benefits: 1) precise images were acquired in a few minutes without special staining; 2) structural irregularity and desmoplastic reactions, which indicated malignancy, were detected; 3) images reflected tissue elasticity, which was statistically comparable among lesions by SOS; 4) follicular functional activity was predictable by converting colloid concentration to SOS; and 5) tumor classification was predictable by SOS because more poorly differentiated carcinomas had a tendency to show lower SOS.

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Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Histological evaluation of thyroid lesions using a scanning acoustic microscope

Miura¹,

Mineta²

2014

PLMI

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“…The SAM used ultrasound to image an object by plotting the speed or attenuation of sound through the sections on the screen (supplemental figures). As harder tissues result in greater SOS values through the sections, SAM can provide data on tissue elasticity and lesions [4,8]. …”

Section: Methodsmentioning

confidence: 99%

“…Scanning acoustic microscopy (SAM) has evoked considerable interest as a method for assessment of both histological and elastic properties using the same section [4,5]. Atomic force microscopy-based nanoindentation is a candidate technique for mechanical testing [3,6].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of aging, diabetes mellitus, and skin wounds by scanning acoustic microscopy with protease digestion

Miura

Yamashita

2018

Pathobiology of Aging & Age-related Diseases

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Scanning acoustic microscopy (SAM) can assess tissue stiffness by calculating the speed of sound (SOS) through tissues. SOS increases as tissue stiffness increases. Sensitivity to protease digestion depends on protein type, concentration, and modification. We analyzed the SOS images of formalin-fixed paraffin-embedded skin sections from elderly, young, diabetic, and nondiabetic subjects, as well as chronic and acute wounds. SAM provided high-resolution histology similar to LM and revealed characteristic SOS alteration following pepsin treatment. SOS values of dermis samples from elderly subjects (especially females) were lower than those of younger adults, which was indicative of age-related dermal softening and loosening. SOS values of elderly females were lower than those of younger females and elderly males. Dermal SOS showed a positive correlation with epidermal thickness. SOS values of epidermis of elderly subjects were higher than those of younger adults and showed a rapid decline 0.5h after protease digestion. Reticular dermis of diabetic patients exhibited greater pepsin resistance than that of nondiabetic patients. Chronic wounds exhibited greater SOS values and pepsin resistance than acute wounds. SOS variation with aging, diabetes mellitus, and wound fibrosis reflected histological and mechanical changes associated with senescence and disease duration. Epidermal thickness reflects age-related changes in dermal stiffness.

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“…A scanning acoustic microscope is a device that uses ultrasound to image an object or tissue 1. The data are digitised and color-coded for display.…”

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confidence: 99%