Pulmonary Immune Response Following Subchronic Stachybotrys chartarum Exposure

Croston, Tara L.; Nayak, Ajay P.; Lemons, Angela R.; Goldsmith, William T.; Germolec, Dori M.; Beezhold, Donald H.; Green, Brett J.

doi:10.1016/j.jaci.2016.12.291

Cited by 2 publications

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“…Several research studies have focused on the miRNA profiles following acute or chronic fungal exposures (Figure 1 ). Table 1 shows 10 studies that have preliminarily characterized differentially expressed miRNAs following exposure to five clinically relevant fungal species including A. fumigatus ( 22 , 90 , 91 ), C. albicans ( 22 , 92 – 94 ), C. neoformans ( 95 ), P. brasiliensis ( 96 , 97 ), and S. chartarum ( 98 ). The paucity of research investigating the regulation of miRNAs on pulmonary and systemic responses to fungal exposure highlights the need for research examining the role miRNAs play in the immunological mechanisms associated with endemic, opportunistic, and environmental fungal exposures.…”

Section: Fungal Exposure: Role Of Mirnasmentioning

confidence: 99%

“…Recent studies have attempted to characterize critical interactions that influence these pulmonary immunological responses. Croston et al found that miR-21a was the only miRNA upregulated in murine whole lung homogenate 48 h following subchronic exposure to S. chartarum ( 98 ) (Figure 2 ). Although miR-21a is known to promote a Th2 phenotype, a more dominant Th1 phenotype was evident.…”

Section: Fungal Exposure: Role Of Mirnasmentioning

confidence: 99%

“…These predictions are primarily based on previously publish datasets derived from a broad diversity of animal models. The miRNA dataset included in Figure 2 was obtained from murine whole lung homogenate 48 h following subchronic exposure to S. chartarum ( 98 ). When analyzing the top diseases and biological functions of the miRNAs included in the dataset, a handful were predicted to be involved in an inflammatory response, specifically in organ inflammation, illustrated in Figure 2 .…”

Section: Fungal Exposure: Role Of Mirnasmentioning

confidence: 99%

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MicroRNA Regulation of Host Immune Responses following Fungal Exposure

et al. 2018

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Fungal bioaerosols are ubiquitous in the environment and human exposure can result in a variety of health effects ranging from systemic, subcutaneous, and cutaneous infections to respiratory morbidity including allergy, asthma, and hypersensitivity pneumonitis. Recent research has focused on the role of microRNAs (miRNAs) following fungal exposure and is overlooked, yet important, group of regulators capable of influencing fungal immune responses through a variety of cellular mechanisms. These small non-coding ribose nucleic acids function to regulate gene expression at the post-transcriptional level and have been shown to participate in multiple disease pathways including cancer, heart disease, apoptosis, as well as immune responses to microbial hazards and occupational allergens. Recent animal model studies have characterized miRNAs following the exposure to inflammatory stimuli. Studies focused on microbial exposure, including bacterial infections, as well as exposure to different allergens have shown miRNAs, such as miR-21, miR-146, miR-132, miR-155, and the let-7 family members, to be involved in immune and inflammatory responses. Interestingly, the few studies have assessed that the miRNA profiles following fungal exposure have identified the same critical miRNAs that have been characterized in other inflammatory-mediated and allergy-induced experimental models. Review of available in vitro, animal and human studies of exposures to Aspergillus fumigatus, Candida albicans, Cryptococcus neoformans, Paracoccidioides brasiliensis, and Stachybotrys chartarum identified several miRNAs that were shared between responses to these species including miR-125 a/b (macrophage polarization/activation), miR-132 [toll-like receptor (TLR)2-mediated signaling], miR-146a (TLR mediated signaling, alternative macrophage activation), and miR-29a/b (natural killer cell function, C-leptin signaling, inhibition of Th1 immune response). Although these datasets provide preliminary insight into the role of miRNAs in fungal exposed models, interpretation of miRNA datasets can be challenging for researchers. To assist in navigating this rapidly evolving field, the aim of this review is to describe miRNAs in the framework of host recognition mechanisms and provide initial insight into the regulatory pathways in response to fungal exposure.

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Section: Fungal Exposure: Role Of Mirnasmentioning

confidence: 99%

Section: Fungal Exposure: Role Of Mirnasmentioning

confidence: 99%

Section: Fungal Exposure: Role Of Mirnasmentioning

confidence: 99%

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MicroRNA Regulation of Host Immune Responses following Fungal Exposure

et al. 2018

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“…Previous data from our laboratory and others demonstrate pulmonary pathology after S. chartarum exposure (24,25,30,31); however, the underlying mechanisms need further clarification. The aim of this study was to characterize the mechanisms contributing to the pulmonary immune responses after repeated exposure to S. chartarum.…”

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confidence: 73%

Inhalation of Stachybotrys chartarum Fragments Induces Pulmonary Arterial Remodeling

Croston

Lemons

Barnes

et al. 2020

Am J Respir Cell Mol Biol

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Stachybotrys chartarum is a fungal contaminant within the built environment and a respiratory health concern in the United States. The objective of this study was to characterize the mechanisms influencing pulmonary immune responses to repeatedly inhaled S. chartarum. Groups of B6C3F1/N mice repeatedly inhaled viable trichothecene-producing S. chartarum conidia (strain A or strain B), heat-inactivated conidia, or high-efficiency particulate absolute-filtered air twice per week for 4 and 13 weeks. Strain A was found to produce higher amounts of respirable fragments than strain B. Lung tissue, serum, and BAL fluid were collected at 24 and 48 hours after final exposure and processed for histology, flow cytometry, and RNA and proteomic analyses. At 4 weeks after exposure, a T-helper cell type 2-mediated response was observed. After 13 weeks, a mixed T-cell response was observed after exposure to strain A compared with a T-helper cell type

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Pulmonary Immune Response Following Subchronic Stachybotrys chartarum Exposure

Cited by 2 publications

References 0 publications

MicroRNA Regulation of Host Immune Responses following Fungal Exposure

MicroRNA Regulation of Host Immune Responses following Fungal Exposure

Inhalation of Stachybotrys chartarum Fragments Induces Pulmonary Arterial Remodeling

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