Pulmonary induction of proinflammatory mediators following the rat exposure to benzo(a)pyrene-coated onto Fe2O3 particles

Garçon, Guillaume; Gosset, Pierre; Garry, Sébastien; Marez, Tine De; Hannothiaux, Marie‐Hélène; Shirali, Pirouz

doi:10.1016/s0378-4274(01)00326-5

Cited by 40 publications

(27 citation statements)

References 36 publications

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“…Total RNA was extracted from liver tissues using an RNeasy mini kit (RNeasy Total RNA System, Qiagen, Courtaboeuf, France), according to the manufacturer's instructions. Conditions for RT were as described previously (20). Briefly, for cDNA synthesis, 1 g of total RNA was suspended in a final volume of 19 l reaction buffer [50 mM Tris ⅐ HCl, 40 mM KCl, 5 mM MgCl 2, 10 mM dithiothreitol, 0.5% Tween 20 (vol/vol), 1 mM dNTP, 20 U RNase inhibitor, and 100 pmol oligo(dT)15 (Roche Diagnostics, Meylan, France)].…”

Section: Study Of Gene Expression Of Cytochrome Cyp 2e1mentioning

confidence: 99%

Low-dose aspirin prevents age-related endothelial dysfunction in a mouse model of physiological aging

Bulckaen¹,

Prévost²,

Boulanger³

et al. 2008

American Journal of Physiology-Heart and Circulatory Physiology

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The age-related impairment of endothelium-dependent vasodilatation contributes to increased cardiovascular risk in the elderly. For primary and secondary prevention, aspirin can reduce the incidence of cardiovascular events in this patient population. The present work evaluated the effect of low-dose aspirin on age-related endothelial dysfunction in C57B/J6 aging mice and investigated its protective antioxidative effect. Agerelated endothelial dysfunction was assessed by the response to acetylcholine of phenylephrine-induced precontracted aortic segments isolated from 12-, 36-, 60-, and 84-wk-old mice. The effect of low-dose aspirin was examined in mice presenting a decrease in endothelial-dependent relaxation (EDR). The effects of age and aspirin treatment on structural changes were determined in mouse aortic sections. The effect of aspirin on the oxidative stress markers malondialdehyde and 8-hydroxy-2Ј-deoxyguanosine (8-OhdG) was also quantified. Compared with that of 12-wk-old mice, the EDR was significantly reduced in 60-and 84-wk-old mice (P Ͻ 0.05); 68-wkold mice treated with aspirin displayed a higher EDR compared with control mice of the same age (83.9 Ϯ 4 vs. 66.3 Ϯ 5%; P Ͻ 0.05). Aspirin treatment decreased 8-OHdG levels (P Ͻ 0.05), but no significant effect on intima/media thickness ratio was observed. The protective effect of aspirin was not observed when treatment was initiated in older mice (96 wk of age). It was found that low-dose aspirin is able to prevent age-related endothelial dysfunction in aging mice. However, the absence of this effect in the older age groups demonstrates that treatment should be initiated early on. The underlying mechanism may involve the protective effect of aspirin against oxidative stress. endothelium; intima/media; vasodilatation; oxidative stress AGE-ASSOCIATED CHANGES SUCH as endothelial dysfunction are involved in the significantly increased risk of cardiovascular complications and microthrombus formation in the elderly patient population (44). The presence of endothelial dysfunction in the coronary or peripheral circulation has been shown to constitute a risk factor for cardiovascular events independent of the development of atherosclerosis or other vascular risk factors (22,(43)(44)(45). There is emerging evidence that age-associated endothelial dysfunction is related to the local formation of reactive oxygen and nitrogen species within and in the vicinity of the vascular wall (13,25,36,43,49). Therapeutic approaches capable of preventing or reversing age-related endothelial dysfunction may thus help to reduce cardiovascular risk in the elderly.Age-related endothelial-dependent relaxation (EDR) decreases in the large vessels of different animal species including humans (36). EDR in response to acetylcholine (ACh) decreases with age in the rat aorta: the maximal relaxation effect of ACh is 100% in 4-to 6-wk-old, 50% in 3-to 6-mo-old, and 25% in 12-to 25-mo-old rats (29). The agerelated decrease in EDR varies from one vessel to another and from one species to anot...

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Section: Study Of Gene Expression Of Cytochrome Cyp 2e1mentioning

confidence: 99%

Low-dose aspirin prevents age-related endothelial dysfunction in a mouse model of physiological aging

Bulckaen¹,

Prévost²,

Boulanger³

et al. 2008

American Journal of Physiology-Heart and Circulatory Physiology

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“…Injury suggesting tumoral induction was observed at only one animal (experimental group 20mg, 12 weeks), which presented a pleomorphism suggesting an adenocarcinoma in situ in the tracheal epithelium. oxidant agent to the carcinogenic ones, induces greater release of pro-inflammatory mediators, contributing to the process of carcinogenesis activation 11 . In three animals of the experimental groups, the presence of BALT hyperplasia was observed.…”

Section: Resultsmentioning

confidence: 99%

“…Models of lung cancer in rats can serve, therefore, as a valuable tool not only to the understanding of the biological bases of these tumors, but also to the development and validation of new interventions and strategies to the cancer treatment, as well as the identification of markers for the early diagnosis 10 . It was opted in this study the injection of the diluted B[a]P by thoracocentesis, when Garçon et al (2001) offers the tracheal instillation as a study option on the PAH effects at the pulmonary tissue 11 . Similar methodology to this study was used by Harrigan et al (2004).…”

Section: Discussionmentioning

confidence: 99%

“…The induction method and the dose used are related as a greatly influent component at the pulmonary carcinogenesis 10 . The analysis of the available literature, references had not been found on the accurate reply-dose of B[a]P enough to the induction of pulmonary carcinogenesis, however it observes that at the studies which outcomes related the instillation of B[a]P to tissular alterations, molecular or genetic in the lungs, the doses had varied between 10mg/kg and 50mg/kg 4,11,12 . It was used coloration by HE for the hystopathological analysis.…”

Section: Discussionmentioning

confidence: 99%

“…Non-observation of the alterations compatible with lung cancer (the exception of an animal of the experimental group 20mg/kg), can be explained by the absence of metabolic activation. Garçon et al (2001) suggest that the exposition to an associated…”

Section: Discussionmentioning

confidence: 99%

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Experimental model of pulmonary carcinogenesis in Wistar rats

Silva¹,

Silva²,

Pereira³

et al. 2007

Acta Cir. Bras.

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Purpose:To elaborate an experimental model of pulmonary carcinogenesis in Wistar rats. Methods: Male Rattus norvegicus albinus, Wistar lineage was carried through an intra-pulmonary instillation of the Benzo[a]pyrene (B[a]P) dilution in alcohol 70%, a polycyclic aromatic hydrocarbon widely known by its power of tumoral induction. Three experimental groups had been formed with 08 animals each: Control Group (Alcohol 70%); B[a]P Group 10 mg/kg; e B[a]P Group 20mg/ kg, submitted to euthanasia 08, 10, 12 and 14 weeks after the experimental procedure. The pulmonary sections had been colored by hematoxilin-eosin (HE) and submitted to the morphometrical analysis to describe the tissue alterations. Results: The presence of diffuse inflammatory alterations was observed in all groups, however, at the analysis of the pulmonary tissue of the experimental groups, it had been observed hyperplasic alterations (BALT hyperplasia), and in one of the animals of the experimental group 20mg/kg (12 weeks), it was noticed the presence of cellular epithelial tracheal pleomorphism, suggesting the adenocarcinoma formation in situ. Conclusion: The main secondary alterations to the intra-pulmonary instillation of B[a]P in Wistar rats were: cellular proliferation, inflammatory alterations of several degrees and nodular lymphoid hyperplasias. The association of an activator agent of the pulmonary metabolic reply is necessary to establish the ideal reply-dose to the development of the lung cancer. Key words: Carcinogenesis. Lung Cancer. Benzo[a]pyrene. Neoplasms. Experimental model. RESUMOObjetivo: Elaborar um modelo experimental de carcinogênese pulmonar em ratos wistar. Métodos: Rattus norvegicus albinus, linhagem Wistar foram submetidos a instilação intra-pulmonar da diluição em álcool 70% de Benzo[a]pireno (B[a]P), um hidrocarboneto aromático policíclico amplamente conhecido por seu poder de indução tumoral. Foram formados três grupos experimentais com 08 animais cada: Grupo Controle (álcool 70%); Grupo B[a]P 10 mg/kg; e Grupo B[a]P 20mg/ kg, submetidos a eutanásia 08, 10, 12 e 14 semanas após o procedimento experimental. As secções pulmonares foram coradas por HE e submetidas a análise morfométrica para descrição das alterações teciduais. Resultados: em todos os grupos observou-se a presença de alterações inflamatórias difusas, porém na análise do tecido pulmonar dos grupos experimentais, observou-se alterações hiperplásicas (hiperplasia de BALT), e em um dos animais do grupo experimental 20mg/kg (12 semanas) notou-se a presença de pleomorfismo celular epitelial traqueal, sugerindo a formação de adenocarcinoma in situ. Conclusão: as principais alterações secundárias à instilação intra-pulmonar de B[a]P em ratos Wistar foram: proliferação celular, alterações inflamatórias de diversos graus e hiperplasias nodulares linfóides. A associação de um agente ativador da resposta metabólica pulmonar pode ser necessária para estabelecimento da dose-resposta ideal ao desenvolvimento do câncer de pulmão. Descritores: Carcinogênese. Câncer de...

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Eisenoxide (einatembare Fraktion) [MAK Value Documentation in German language, 2011]

2012

The MAK‐Collection for Occupational Health and Safety

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Veröffentlicht in der Reihe Gesundheitsschädliche Arbeitsstoffe , 51. Lieferung, Ausgabe 2011 Der Artikel enthält folgende Kapitel: Allgemeine Charakterisierung Vorkommen Lagerstätten und Hauptförderländer Herstellung und Verwendung Rohstoffe Herstellungs‐ und Verarbeitungsprozess Herstellung synthetischer Eisenoxide (Pigmente) Verwendungsgebiete ― Eisenoxide Messverfahren Allgemeiner Wirkungscharakter Wirkungsmechanismus Toxikokinetik und Metabolismus Aufnahme, Verteilung, Ausscheidung Metabolismus Erfahrungen beim Menschen Einmalige Exposition Wiederholte Exposition Wirkung auf Haut und Schleimhäute Allergene Wirkung Reproduktionstoxizität Genotoxizität Kanzerogenität Tierexperimentelle Befunde und In‐vitro‐Untersuchungen Akute Toxizität Subakute, subchronische und chronische Toxizität Wirkung auf Haut und Schleimhäute Allergene Wirkung Reproduktionstoxizität Genotoxizität Kanzerogenität Sonstige Wirkungen Bewertung

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Pulmonary induction of proinflammatory mediators following the rat exposure to benzo(a)pyrene-coated onto Fe2O3 particles

Cited by 40 publications

References 36 publications

Low-dose aspirin prevents age-related endothelial dysfunction in a mouse model of physiological aging

Low-dose aspirin prevents age-related endothelial dysfunction in a mouse model of physiological aging

Experimental model of pulmonary carcinogenesis in Wistar rats

Eisenoxide (einatembare Fraktion) [MAK Value Documentation in German language, 2011]

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