Pulmonary Toxicity of Instilled Silver Nanoparticles: Influence of Size, Coating and Rat Strain

Seiffert, Joanna; Hussain, Faheem; Wiegman, Coen; Li, Feng; Bey, Leo; Baker, Warren; Porter, Alexandra E.; Ryan, Mary P.; Chang, Yan; Gow, Andrew J.; Zhang, Junfeng Jim; Zhu, Jie; Tetley, TD; Chung, Kian Fan

doi:10.1371/journal.pone.0119726

Cited by 109 publications

(95 citation statements)

References 43 publications

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“…Since the compositions of the ambient Ku, Ir, and NIST dusts differ somewhat from one another and from TiO 2 particles, it seems likely that much, if not all, of the early inflammatory response observed in this study is related to particle deposition rather than to composition of particles. As a further example, a similar time course of inflammatory response was recently reported for nanosilver particles, another compositionally unrelated material (Seiffert et al 2015). …”

Section: Discussionsupporting

confidence: 72%

Evaluation of the Pulmonary Toxicity of Ambient Particulate Matter From Camp Victory, Iraq

Porter

Green

Harley

et al. 2015

Journal of Toxicology and Environmental Health, Part A

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Anecdotal reports in the press and epidemiological studies suggest that deployment to Iraq and Afghanistan may be associated with respiratory diseases and symptoms in U.S. military personnel and veterans. Exposures during military operations were complex, but virtually all service members were exposed to high levels of respirable, geogenic dust. Inhalation of other dusts has been shown to be associated with adverse health effects, but the pulmonary toxicity of ambient dust from Iraq has not been previously studied. The relative toxicity of Camp Victory dust was evaluated by comparing it to particulate matter from northern Kuwait, a standard U.S. urban dust, and crystalline silica using a single intratracheal instillation in rats. Lung histology, protein levels, and cell counts were evaluated in the bronchoalveolar lavage fluid 1–150 d later. The Iraq dust provoked an early significant, acute inflammatory response. However, the level of inflammation in response to the Iraq dust, U.S. urban dust, and Kuwait dust rapidly declined and was nearly at control levels by the end of the study At later times, animals exposed to the Iraq, U.S. urban, or Kuwait dusts showed increased small airway remodeling and emphysema compared to silica-exposed and control animals without evidence of fibrosis or premalignant changes. The severity and persistence of pulmonary toxicity of these three dusts from the Middle East resemble those of a U.S. urban dust and are less than those of silica. Therefore, Iraq dust exposure is not highly toxic, but similar to other poorly soluble low-toxicity dusts.

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Section: Discussionsupporting

confidence: 72%

Evaluation of the Pulmonary Toxicity of Ambient Particulate Matter From Camp Victory, Iraq

Porter

Green

Harley

et al. 2015

Journal of Toxicology and Environmental Health, Part A

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“…Such an effect is obviously more significant for small NPs, which appear to be the most proinflammatory particles. 44 It is found that with the PS corona, diameter of the Ag-NP increases from 5 to 14.8 nm ( Table 1). The lungs are the major organ targeted by prolonged exposure to Ag-NPs.…”

Section: Resultsmentioning

confidence: 94%

Unveiling the Molecular Structure of Pulmonary Surfactant Corona on Nanoparticles

et al. 2017

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The growing risk of human exposure to airborne nanoparticles (NPs) causes a general concern on the biosafety of nanotechnology. Inhaled NPs can deposit in the deep lung at which they interact with the pulmonary surfactant (PS). Despite the increasing study of nano-bio interactions, detailed molecular mechanisms by which inhaled NPs interact with the natural PS system remain unclear. Using coarse-grained molecular dynamics simulation, we studied the interaction between NPs and the PS system in the alveolar fluid. It was found that regardless of different physicochemical properties, upon contacting the PS, both silver and polystyrene NPs are immediately coated with a biomolecular corona that consists of both lipids and proteins. Structure and molecular conformation of the PS corona depend on the hydrophobicity of the pristine NPs. Quantitative analysis revealed that lipid composition of the corona formed on different NPs is relatively conserved and is similar to that of the bulk phase PS. However, relative abundance of the surfactant-associated proteins, SP-A, SP-B, and SP-C, is notably affected by the hydrophobicity of the NP. The PS corona provides the NPs with a physicochemical barrier against the environment, equalizes the hydrophobicity of the pristine NPs, and may enhance biorecognition of the NPs. These modifications in physicochemical properties may play a crucial role in affecting the biological identity of the NPs and hence alter their subsequent interactions with cells and other biological entities. Our results suggest that all studies of inhalation nanotoxicology or NP-based pulmonary drug delivery should consider the influence of the PS corona.

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“…As for chemokines other than CINC, Keratinocyte derived chemokines (KC) have been reported to increase persistently in lung following intratracheal instillation of quartz. Seiffert et al [27] conducted an intratracheal instillation test of silver nanoparticles, and the exposure induced a transient KC concentration in BALF, while the chemokine expression pattern was accompanied by neutrophil influx in the lung. An inhalation exposure of ZnO nanoparticles with low toxicity did not induce neutrophil influx or an increase the concentration of KC in BALF [28].…”

Section: Endpointsmentioning

confidence: 99%

Significance of Intratracheal Instillation Tests for the Screening of Pulmonary Toxicity of Nanomaterials

Morimoto

Izumi

Yoshiura

et al. 2017

J UOEH

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: Inhalation tests are the gold standard test for the estimation of the pulmonary toxicity of respirable materials. Intratracheal instillation tests have been used widely, but they yield limited evidence of the harmful effects of respirable materials. We reviewed the effectiveness of intratracheal instillation tests for estimating the hazards of nanomaterials, mainly using research papers featuring intratracheal instillation and inhalation tests centered on a Japanese national project. Compared to inhalation tests, intratracheal instillation tests induced more acute inflammatory responses in the animal lung due to a bolus effect regardless of the toxicity of the nanomaterials. However, nanomaterials with high toxicity induced persistent inflammation in the chronic phase, and nanomaterials with low toxicity induced only transient inflammation. Therefore, in order to estimate the harmful effects of a nanomaterial, an observation period of 3 months or 6 months following intratracheal instillation is necessary. Among the endpoints of pulmonary toxicity, cell count and percentage of neutrophil, chemokines for neutrophils and macrophages, and oxidative stress markers are considered most important. These markers show persistent and transient responses in the lung from nanomaterials with high and low toxicity, respectively. If the evaluation of the pulmonary toxicity of nanomaterials is performed in not only the acute but also the chronic phase in order to avoid the bolus effect of intratracheal instillation and inflammatory-related factors that are used as endpoints of pulmonary toxicity, we speculate that intratracheal instillation tests can be useful for screening for the identification of the hazard of nanomaterials through pulmonary inflammation.

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Pulmonary Toxicity of Instilled Silver Nanoparticles: Influence of Size, Coating and Rat Strain

Cited by 109 publications

References 43 publications

Evaluation of the Pulmonary Toxicity of Ambient Particulate Matter From Camp Victory, Iraq

Evaluation of the Pulmonary Toxicity of Ambient Particulate Matter From Camp Victory, Iraq

Unveiling the Molecular Structure of Pulmonary Surfactant Corona on Nanoparticles

Significance of Intratracheal Instillation Tests for the Screening of Pulmonary Toxicity of Nanomaterials

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