Pulp fruit added to culture medium for in vitro orchid development

Souza, Gilberto Rostirolla Batista de; Lone, Alessandro Borini; Faria, Ricardo Tadeu de; Oliveira, Karen Sinéia de

doi:10.5433/1679-0359.2013v34n3p1141

Cited by 3 publications

(5 citation statements)

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“…The combination of VW and 150 g L -1 of the banana extract (BE) was the best regeneration medium for D. Dian Agrihorti EC to produce the best performances of plantlets. Optimal regeneration indicated via better shoot and root growth was due to banana nutrition contents, i.e., glucose, carbohydrate, hormones, and vitamins of A, B, C, E, and K involving B1 or thiamine (De Souza et al, 2013;Bayraktar et al, 2015;Hapsari and Lestari, 2016;Mustika and Semiarti, 2021). Thiamine is a type of vitamin B1 that can stimulate cell division in the shoot and root so that the shoots and roots can grow faster due to its important role as a cofactor in metabolism activities and regulatory system (Subki et al, 2010;De Souza et al, 2013;Humaira et al, 2015).…”

Section: Discussion Discussion Discussionmentioning

confidence: 99%

“…Optimal regeneration indicated via better shoot and root growth was due to banana nutrition contents, i.e., glucose, carbohydrate, hormones, and vitamins of A, B, C, E, and K involving B1 or thiamine (De Souza et al, 2013;Bayraktar et al, 2015;Hapsari and Lestari, 2016;Mustika and Semiarti, 2021). Thiamine is a type of vitamin B1 that can stimulate cell division in the shoot and root so that the shoots and roots can grow faster due to its important role as a cofactor in metabolism activities and regulatory system (Subki et al, 2010;De Souza et al, 2013;Humaira et al, 2015). Vitamins are also necessary for the growth of cells that are still actively dividing because they act as catalysts in metabolism (Humaira et al, 2015;Hapsari and Lestari, 2016).…”

Section: Discussion Discussion Discussionmentioning

confidence: 99%

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Optimizing the potential utilization of bioreactors for the mass propagation of Indonesian Dendrobium varieties

RACHMAWATI,

PRAMANIK,

SHINTIAVIRA

et al. 2024

Not Bot Horti Agrobo

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The study focuses on two Indonesia Dendrobium varieties, D. Dian Agrihorti (DDA) and D. Syifa Agrihorti (DSA), which have potential to be commercialized, but face limitations on the availability of qualified seedlings sustainably. The research aimed to establish an in vitro propagation protocol using a Temporary Immersion System (TIS) to produce high-quality seedlings efficiently. Various factors, including varieties, media, plant growth regulators, anti-phenol compounds, organic additives, and TIS settings, were investigated using the basal part of the plantlet as the explant source. Key findings revealed that DDA outperformed DSA across all observed variables. In the initiation phase, basal plantlets cultured in Murashige and Skoog (MS) medium supplemented with 1.0 mg L-1 thidiazuron and 0.5 mg L-1 N-6 benzylaminopurine enhanced embryogenic callus (EC) formation, with a 13.5-day initiation period, 72% potential explant growth, 0.41 cm callus size, and a 3.45 rate of multiplication. During the proliferation stage, the addition of 150 mg L-1 ascorbic acid (AC) and the application of a TIS with a 30-minute dry period and a 15-minute wet period resulted in a 515.5% increase in EC fresh weight for DDA accompanied by a 6.16 multiplication rate. Regeneration of shoots was achieved using a Vacin and Went medium with 150 g L-1 banana extract, yielding 29.2 shoots per clump. Subsequent rooting of the shoots in 2 g L-1 Hyponex® medium with 20 g L-1 sugar and 2% AC proved successful. Acclimatization of plantlets with Cycas rumpii bulk demonstrated a 100% survivability rate. The established propagation protocol for DDA holds significant potential for application to other Dendrobium varieties, offering a sustainable and efficient method for meeting commercial demands in the Indonesian market.

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Section: Discussion Discussion Discussionmentioning

confidence: 99%

Section: Discussion Discussion Discussionmentioning

confidence: 99%

Optimizing the potential utilization of bioreactors for the mass propagation of Indonesian Dendrobium varieties

RACHMAWATI,

PRAMANIK,

SHINTIAVIRA

et al. 2024

Not Bot Horti Agrobo

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“…For roots fresh and dry mass, superior values were observed for the simplified medium, under light source L4. In a study with simplified culture media with fruit pulps, for the in vitro growth of Cattleya bicolor, Souza et al (2013) found no differences for roots and canopy dry mass when comparing the ½ MS medium to the simplified medium composed by 'Nanica' banana pulp. This shows that the reaction to growth also depends on the interaction between species and culture medium.…”

Section: Resultsmentioning

confidence: 99%

“…In the in vitro propagation of orchids, the modified MS medium (MURASHIGE;SKOOG, 1962) has been used by most plants biofactories (FARIA et al, 2002;MORAES et al, 2005), as well as several simplified media based on commercial fertilizers and organic components such as banana pulp, tomato pulp and coconut water (ARAÚJO et al, 2006;STANCATO et al, 2008;BAQUE et al, 2011;COLOMBO et al, 2012;SU et al, 2012;SOUZA et al, 2013;FAVETTA et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Light sources and culture media in the in vitro growth of the Brazilian orchid Microlaelia lundii

Favetta

Colombo

Júnior

et al. 2017

SCA

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Light sources and culture media in the in vitro growth of the Brazilian orchid Microlaelia lundii AbstractThe Brazilian micro-orchid Microlaelia lundii enchants by its beautiful blooms in winter, but its cultivation is still a challenge, requiring specific care. The objective of this work was to evaluate the influence of light sources and culture media on in vitro growth and ex vitro survival of this micro-orchid. M. lundii seedlings were transferred to flasks containing the culture medium ½ MS and Simplified Medium, consisting of 5 mL L -1 of Biofert Plus® NPK fertilizer (09-08-09) and 60 g L -1 of 'Nanica' banana pulp. These flasks were placed in a growth chamber composed of seven environments, characterized by types of lighting: L1) LEDtube 4000K; L2) LEDtube 6500K; L3) L1 + L2; L4) L1 + L1; L5) red LED; L6) blue LED; L7) fluorescent lamp (control). After 200 days of cultivation, biometric growth parameters and leaves chlorophyll content were evaluated together with the percentage of seedling survival when acclimatized. At the end of the experiment, an analysis of individual variances and a combined analysis of the environments were carried out. The simplified medium led to higher seedlings, especially for light source L6. Light sources L1, L2, L3 and L4 promoted an increase in the seedlings root system in the simplified medium. As for the chlorophyll content, L4 and L7 showed higher contents of chlorophyll a and b. The simplified medium led practically to 100% of seedling survival, regardless of the light source used. Results shown that the simplified medium under light sources L1, L2, L3 and L4 is recommended for the in vitro propagation of M. lundii. Key words: LED. Nutritious medium. Orchidaceae. Quality of light. ResumoA micro-orquídea brasileira Microlaelia lundii encanta pelas belas floradas no inverno, no entanto o seu cultivo ainda é um desafio, requerendo cuidados específicos. Assim, objetivou-se avaliar a influência de fontes de luz e meios de cultura no crescimento in vitro e na sobrevivência ex vitro dessa microorquídea. Plântulas de M. lundii foram transferidas para frascos contendo os meios de cultura: ½ MS e Meio Simplificado, composto por 5 mL L -1 do fertilizante Biofert Plus ® NPK (08-09-09) e 60 g L -1de polpa de banana 'Nanica'. Estes frascos foram acondicionados em câmara de cultivo composta por sete ambientes, caracterizados pelo tipo de iluminação: L1) LEDtube 4000K; L2) LEDtube 6500K; L3) L1 + L2; L4) L1 + L1; L5) LED vermelho; L6) LED azul; L7) Lâmpada fluorescente (controle o teor de clorofila nas folhas. Também, avaliou-se a porcentagem de sobrevivência das plantas, quando aclimatizadas. O meio simplificado propiciou plântulas mais altas, com destaque para a fonte de luz L6. As fontes de luz L1, L2, L3 e L4 no meio simplificado proporcionaram aumento do sistema radicular das plântulas. Quanto ao teor de clorofila, L4 e L7 apresentaram maiores teores de clorofila a e b. O meio simplificado propiciou, praticamente, 100% de sobrevivência das plantas, independente da fonte de luz util...

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“…Several studies reported that the addition of some organic substances in medium culture, such as fruit extract can enhance plantlet growth. The fruit extracts in culture medium contain many nutrients providing hormones and plant growth regulator useful to increase plant growth (Souza et al 2013). The addition of banana extract (50 g/L) in culture medium has been shown to promote protocormlike bodies (PLBs) viability (Yulianti et al 2016) Musa acuminata x Musa balbisiana is one of the Indonesian banana cultivars called "Pisang Raja" that Indonesian people widely consume.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Culture of Phalaenopsis amabilis (L.) Blume Orchid for Seedling Production with Banana Extract Supplementation and Light Treatment for Ex Situ Conservation

Arum

Semiarti

2022

J.Tropical Biodiversity Biotechnology

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In vitro culture is one of the effective cultivation methods for seedling production of orchids that can be used as a powerful tool in the conversation of orchids. The aims of the present study were to (i) investigate the effects of the addition of banana extract (0 g/l, 100 g/l, and 150 g/l) on media (NP media), in two different light regimes (light and dark conditions) on the growth of plantlets of an epiphytic orchid Phalaenopsis amabilis in in vitro culture. Methods used included (i) subculturing orchid seedlings in treatments media, (ii) measuring leaves and roots chlorophyll content and growth parameters, (iii) anatomical preparation of leaves and roots of the seedlings. The results showed that the best condition for getting greater seedlings of P. amabilis plantlets is in media with an addition of 100 g/L banana extract in light condition. The highest amount of chlorophyll in the P. amabilis leaves was found in medium with the addition of 100 g/L banana extract medium in light conditions. The thickness of mesophyll and the largest root diameter of P. amabilis seedlings were also found in media with the addition of 100 g/L banana extract medium in light condition. In conclusion, the addition of 100gr/L banana extract into basic culture medium will be beneficial for seedlings production of P. amabilis with great appearance, for ex situ orchid conservation programs.

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Pulp fruit added to culture medium for in vitro orchid development

Cited by 3 publications

References 5 publications

Optimizing the potential utilization of bioreactors for the mass propagation of Indonesian Dendrobium varieties

Optimizing the potential utilization of bioreactors for the mass propagation of Indonesian Dendrobium varieties

Light sources and culture media in the in vitro growth of the Brazilian orchid Microlaelia lundii

<i>In Vitro</i> Culture of <i>Phalaenopsis amabilis</i> (L.) Blume Orchid for Seedling Production with Banana Extract Supplementation and Light Treatment for <i>Ex Situ</i> Conservation

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