Pulp response of mineral trioxide aggregate, calcium sulfate or calcium hydroxide

Objectives: This study investigated changes in gene expressions concerning of differentiation, proliferation, mineralization and inflammation using Human-8 expression bead arrays when white Mineral Trioxide Aggregate and calcium hydroxide-containing cement were applied in vitro to human dental pulp cells (HDPCs). Materials and Methods: wMTA (white ProRoot MTA, Dentsply) and Dycal (Dentsply Caulk) in a Teflon tube (inner diameter 10 mm, height 1 mm) were applied to HDPCs. Empty tube-applied HDPCs were used as negative control. Total RNA was extracted at 3, 6, 9 and 24 hr after wMTA and Dycal application. The results of microarray were confirmed by reverse transcriptase polymerase chain reaction. Results: Out of the 24,546 genes, 43 genes (e.g., BMP2, FOSB, THBS1, EDN1, IL11, COL10A1, TUFT1, HMOX1) were up-regulated greater than two-fold and 25 genes (e.g., SMAD6, TIMP2, DCN, SOCS2, CEBPD, KIAA1199) were down-regulated below 50% by wMTA. Two hundred thirty nine genes (e.g., BMP2, BMP6, SMAD6, IL11, FOS, VEGFA, PlGF, HMOX1, SOCS2, CEBPD, KIAA1199) were up-regulated greater than two-fold and 358 genes (e.g., EDN1, FGF) were down-regulated below 50% by Dycal. Conclusions: Both wMTA and Dycal induced changes in gene expressions related with differentiation and proliferation of pulp cells. wMTA induced changes in gene expressions related with mineralization, and Dycal induced those related with angiogenesis. The genes related with inflammation were more expressed by Dycal than by wMTA. It was confirmed that both wMTA and Dycal were able to induce gene expression changes concerned with the pulp repair in different ways.

Section: 서 론unclassified

Comparison of gene expression profiles of human dental pulp cells treated with mineral trioxide aggregate and calcium hydroxide

Kim

Shon

Lee

et al. 2011

“…그러나, 인간 치수세포의 분화에 대한 β -glycerophosphate와 dexamethasone의 적정 농도는 아 직 보고된 바가 없다. 직접 치수 복조에서 MTA가 수산화칼슘보다 더 생체 친 화적이며 6) , 세포 증식을 증가시키며 7) , 수중에서 장기간 용 해되면서 수산화칼슘을 방출하여 주변 조직의 석회화를 촉 진한다는 보고가 있다 8,9) . MTA의 주요 구성물은 Portland cement와 bismuth oxide이고 10) , bismuth oxide는 방사 선 불투과성을 증가시키기 위해 첨가된다.…”

unclassified

A bioactivity study of Portland cement mixed with β-glycerophosphosphate on human pulp cell

Jang

Cho

2009

The purpose of this study is to investigate the response of human pulp cell on Portland cement mixed with β -glycerophosphate. To investigate the effect of β -glycerophosphate and/or dexamethasone on human pulp cell, ALP activity on various concentration of β -glycerophosphate and dexamethasone was measured and mineral nodule of human pulp cell was stained with Alizarin red S. MTS assay and ALP activity of human pulp cell on Portland cement mixed with various concentration of β -glycerophosphate (10 mM, 100mM, 1M) was measured and the specimens were examined under SEM.Addition of β -glycerophosphate or dexamethasone alone had no effect however, the addition of 5 mM β -glycerophosphate and 100 nM dexamethasone had the largest increasement in ALP activity. There was no toxicity in all samples and the data showed that Portland cement mixed with 10 mM β -glycerophosphate had more increase in ALP activity compared with control.In conclusion, Portland cement mixed with β -glycerophosphate has no toxicity and promotes differentiation and mineralization of pulp cell compared with additive-free Portland cement. This implicated that application of Portland cement mixed with β -glycerophosphate might form more reparative dentin and in turn it would bring direct pulp capping to success. [J Kor Acad Cons Dent 34(5): [415][416][417][418][419][420][421][422][423] 2009]

“…8 In animal studies, MTA proved to be more biocompatible to pulp tissue than calcium hydroxide and calcium sulfate as pulp capping material. 9 MTA was also the only material studied that allowed cementum overgrowth. 10,11 In vitro studies of human osteoblasts showed that MTA stimulated cytokine release and interleukin production.…”

Section: Introductionmentioning

confidence: 99%

“…3 The biocompatibility of MTA has been assessed in many studies. [3][4][5][6][7][8][9][10][11][12][13][14] Calcium hydroxide which is biocompatible with tissue, is the main compound released by MTA in water. 3 The results of an in vitro study showed that MTA was less toxic than amalgam, SuperEBA and IRM.…”

Section: Introductionmentioning

confidence: 99%

Biocompatibility of experimental mixture of mineral trioxide aggregate and glass ionomer cement

Jeong

Bae

et al. 2010

Self Cite

Objectives:The purpose of the present in vitro study was to evaluate the biocompatibility of mineral trioxide aggregate (MTA) mixed with glass ionomer cement (GIC), and to compare it with that of MTA, GIC, IRM and SuperEBA. Materials and Methods: Experimental groups were divided into 3 groups such as 1 : 1, 2 : 1, and 1 : 2 groups depending on the mixing ratios of MTA powder and GIC powder. Instead of distilled water, GIC liquid was mixed with the powder. This study was carried out using MG-63 cells derived from human osteosarcoma. They were incubated for 1 day on the surfaces of disc samples and examined by scanning electron microscopy. To evaluate the cytotoxicity of test materials quantitatively, XTT assay was used. The cells were exposed to the extracts and incubated. Cell viability was recorded by measuring the optical density of each test well in reference to controls. Results: The SEM revealed that elongated, dense, and almost confluent cells were observed in the cultures of MTA mixed with GIC, MTA and GIC. On the contrary, cells on the surface of IRM or SuperEBA were round in shape. In XTT assay, cell viability of MTA mixed with GIC group was similar to that of MTA or GIC at all time points. IRM and SuperEBA showed significantly lower cell viability than other groups at all time points (p < 0.05).