Pulsatile pump perfusion of pancreata before human islet cell isolation

Leeser, David B.; Bingaman, Adam W.; Полякова, Л А; Shi, Qingqing; Gage, Fred; Bartlett, S; Farney, Alan C.

doi:10.1016/j.transproceed.2004.04.041

Cited by 36 publications

(29 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, biopsying the human pancreas following in vivo imaging is clinically unacceptable. A reference model might instead be whole human perfused pancreas obtained from brain-dead donors [45], although the few available pancreases are badly needed for treatment of disease via whole organ or islet transplantation. A more feasible approach is to study patients for whom resection of pancreatic tumour is planned.…”

Section: Next Steps For Clinical Validationmentioning

confidence: 99%

In vivo imaging of beta cells with radiotracers: state of the art, prospects and recommendations for development and use

et al. 2016

View full text Add to dashboard Cite

Radiotracer imaging is characterised by high in vivo sensitivity, with a detection limit in the lower picomolar range. Therefore, radiotracers represent a valuable tool for imaging pancreatic beta cells. High demands are made of radiotracers for in vivo imaging of beta cells. Beta cells represent only a small fraction of the volume of the pancreas (usually 1-3%) and are scattered in the tiny islets of Langerhans throughout the organ. In order to be able to measure a beta cell-specific signal, one has to rely on highly specific tracer molecules because current in vivo imaging technologies do not allow the resolution of single islets in humans non-invasively. Currently, a considerable amount of preclinical data are available for several radiotracers and three are under clinical evaluation. We summarise the current status of the evaluation of these tracer molecules and put forward recommendations for their further evaluation.

show abstract

Section: Next Steps For Clinical Validationmentioning

confidence: 99%

In vivo imaging of beta cells with radiotracers: state of the art, prospects and recommendations for development and use

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Earlier studies have demonstrated that pancreas hypothermic preservation by machine perfusion is feasible and can be safely extended to 24 and 48 hours [6;47;98;172;173]. Dedicated renal perfusion systems have been employed mostly [47;98;172;173] after appropriate modifications required to accommodate the physiologic low flow and pressure needs of the pancreas [6].…”

Section: Hmp Of Other Transplant Organs (Heart Liver and Pancreas)mentioning

confidence: 99%

“…Dedicated renal perfusion systems have been employed mostly [47;98;172;173] after appropriate modifications required to accommodate the physiologic low flow and pressure needs of the pancreas [6]. The latter helps avoid excessive organ edema that post-segmental transplantation and reperfusion has been documented to result in sub-capsular bleeding, hemorrhagic necrosis, venous congestion, and hemorrhagic pancreaticoduodenal secretions [172].…”

Section: Hmp Of Other Transplant Organs (Heart Liver and Pancreas)mentioning

confidence: 99%

Current state of hypothermic machine perfusion preservation of organs: The clinical perspective

2010

View full text Add to dashboard Cite

This review focuses on the application of hypothermic perfusion technology as a topic of current interest with the potential to have a salutary impact on the mounting clinical challenges to improve the quantity and quality of donor organs and the outcome of transplantation. The ex vivo perfusion of donor organs on a machine prior to transplant, as opposed to static cold storage on ice, is not a new idea but is being re-visited because of the prospects of making available more and better organs for transplantation. The rationale for pursuing perfusion technology will be discussed in relation to emerging data on clinical outcomes and economic benefits for kidney transplantation. Reference will also be made to on-going research using other organs with special emphasis on the pancreas for both segmental pancreas and isolated islet transplantation. Anticipated and emerging benefits of hypothermic machine perfusion of organs are: i) maintaining the patency of the vascular bed, ii) providing nutrients and low demand oxygen to support reduced energy demands, iii) removal of metabolic by-products and toxins, iv) provision of access for administration of cytoprotective agents and/or immunomodulatory drugs, v) increase of available assays for organ viability assessment and tissue matching, vi) facilitation of a change from emergency to elective scheduled surgery with reduced costs and improved outcomes, vii) improved clinical outcomes as demonstrated by reduced PNF and DGF parameters, viii) improved stabilization or rescue of ECD kidneys or organs from NHBD that increase the size of the donor pool, ix) significant economic benefit for the transplant centers and reduced health care costs, and x) provision of a technology for ex vivo use of non-transplanted human organs for pharmaceutical development research.

show abstract

“…The majority of studies aimed to optimize the preservation of pancreata in order to increase the islet yield after cold preservation [31,32]. In a very small human study, machine perfusion of pancreata resulted in better islet yield and viability compared with SCS [33]. The same devices currently available for kidney perfusion can be used and adapted for the perfusion of human pancreata.…”

Section: Machine Perfusion: Experience and Devices For Clinical Usementioning

confidence: 99%

Machine perfusion in organ transplantation

Raemdonck

Neyrinck²,

Rega³

et al. 2013

Current Opinion in Organ Transplantation

View full text Add to dashboard Cite

show abstract

Pulsatile pump perfusion of pancreata before human islet cell isolation

Cited by 36 publications

References 8 publications

In vivo imaging of beta cells with radiotracers: state of the art, prospects and recommendations for development and use

In vivo imaging of beta cells with radiotracers: state of the art, prospects and recommendations for development and use

Current state of hypothermic machine perfusion preservation of organs: The clinical perspective

Machine perfusion in organ transplantation

Contact Info

Product

Resources

About