In this paper, we have explored the pulsating flow of a magnetohydrodynamic (MHD) micropolar nanofluid in a vertical channel by applying Buongiorno’s nanofluid model with entropy analysis. The effects of Brownian motion, thermophoresis, Joule heating (Ohmic heating), and thermal radiation are taken into account. The specified concept is significant in the fields of polymer engineering, cancer therapy, and nano-drug delivery. The perturbation approach is applied to convert the governing partial differential equations (PDEs) into ordinary differential equations (ODEs) and cracked numerically by utilizing the shooting process via Runge-Kutta fourth-order method. The flow influences of velocity, microrotation, temperature, nanoparticle concentration, entropy generation, and Bejan number are deliberated by plotting graphs and analyzed in detail for the various values of emerging physical parameters. The heat and mass transfer rates are given in a table.