Pulse-field-gel-electrophoresis development for Salmonella species using PaeR7 I enzyme

Abstract

Pulse field gel electrophoreSIS (PFGE) currently has been preformed on 22 Salmonella spp. isolates using a new method that distinguishes between different spec1es of Salmonella and g1ves reproducible results using Pae R7 1 enzyme. This PFGE data, when using the Pae R7 1 enzyme, was compared to the Xba 1 restriction enzyme that is used by CDC (Center for Disease Control) for Salmonella spp 1solate compansons. PFGE results were analyzed usmg cluster analys1s and results were comparable between Pae R7 1 and Xba 1 enzymes for dist1ngu1shmg differences