Pulse generators for pulsed electric field exposure of biological cells and tissues

Behrend, Matthew R.; Kuthi, A.; Gu, Xianyue; Vernier, P. Thomas; Marcu, Laura; Craft, Cheryl M.; Gundersen, M.A.

doi:10.1109/tdei.2003.1237331

Cited by 71 publications

(33 citation statements)

References 4 publications

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“…These nanosecond pulsed electric fields (nsPEFs)have also shown great promise for treating cancer [31,32] by promoting calcium bursts [33], translocation of phosphatidylserine [33], and DNA damage [34], all of which are capable of triggering an apoptotic cascade. Typically, the pulse generator is based on a spark gap switched transmission line, and the resulting output closely resembles a unipolar pulse [35,36]. For unipolar pulses of this nature, cell death can be induced with a significant drop in muscle contractions as compared to unipolar microsecond-long pulses [23].…”

Section: Current State Of the Artmentioning

confidence: 99%

Advances in Therapeutic Electroporation to Mitigate Muscle Contractions

Arena¹,

Davalos²

2012

J Membra Sci Technol

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Section: Current State Of the Artmentioning

confidence: 99%

Advances in Therapeutic Electroporation to Mitigate Muscle Contractions

Arena¹,

Davalos²

2012

J Membra Sci Technol

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“…A different, more recently implemented architecture for both aeronautical combustion and biological applications uses resonant charging, magnetic compression, and a diode opening switch for pulse sharpening ( Figure 2). [17,31,40], intracellular calcium release [1], chromatin changes (Figure 3), and diagnostic indicators of apoptosis like caspase activation, PARP cleavage, nuclear condensation, and loss of mitochondrial membrane potential [37]. These phenomena have been observed in our laboratories in living cells during pulse exposures in microfabricated electrode chambers [39] and in electroporation cuvettes.…”

Section: Microfluidic Channels Separated By Pulse-gated Membranesmentioning

confidence: 69%

“…Ideally they will provide the operator with considerable flexibility in pulse amplitude, duration, and repetition rate. Pulse generators for biological investigations developed at USC include MOSFET-and fast recovery diode-based systems designed to drive cell suspensions in a microscope slide electrode microchamber for observations of living cells during pulse exposure with pulse durations from 3 ns to 30 ns and electric fields from I MV/m to 10 MV/m [37,38,39]. A different, more recently implemented architecture for both aeronautical combustion and biological applications uses resonant charging, magnetic compression, and a diode opening switch for pulse sharpening ( Figure 2).…”

Section: Microfluidic Channels Separated By Pulse-gated Membranesmentioning

confidence: 99%

High-Order Quadratures for the Solution of Scattering Problems in Two Dimensions

Duan¹,

Rokhlin²

2008

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“…2009.2027422 microseconds up to several milliseconds. Recently, a new type of electroporators named nanoporators was developed and used in biology, generating electric pulses of only a few nanoseconds duration [5], [6]. The design of a nanoporator is usually based on a Blumlein generator.…”

Section: Introductionmentioning

confidence: 99%

“…Blumlein generators operated by very fast switches are adequate for generating pulses having a short rise time, and consequently, for generating pulses of short duration. The duration of the pulse generated by means of a basic Blumlein generator depends on the length of the transmission line only and is, therefore, of a fixed duration [5], [6].…”

Section: Introductionmentioning

confidence: 99%

Blumlein Configuration for High-Repetition-Rate Pulse Generation of Variable Duration and Polarity Using Synchronized Switch Control

Reberšek

Kranjc

Pavliha

et al. 2009

IEEE Trans. Biomed. Eng.

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Abstract-Blumlein generators are used in different applications such as radars, lasers, and also recently in various biomedical studies, where the effects of high-voltage nanosecond pulses on biological cells are evaluated. In these studies, it was demonstrated that by applying high-voltage nanosecond pulses to cells, plasma membrane and cell organelles are permeabilized. As suggested in a recent publication, the repetition rate and polarity of nanosecond high-voltage pulses could have an important effect on the electropermeabilization process, and consequently, on the observed phenomena. Therefore, we designed a new Blumlein configuration that enables a higher repetition rate of variable duration of either bipolar or unipolar high-voltage pulses. We achieved a maximal pulse repetition rate of 1.1 MHz. However, theoretically, this rate could be even higher. We labeled endocytotic vesicles with lucifer yellow and added propidium iodide to a cell suspension for testing the cell plasma membrane integrity, so we were able to observe the permeabilization of endocytotic vesicles and the cell plasma membrane at the same time. The new design of pulse generator was built, verified, and also tested in experiments. The resulting flexibility and variability allow further in vitro experiments to determine the importance of the pulse repetition rate and pulse polarity on membrane permeabilization-both of the cell plasma membrane as well as of cell organelle membranes.

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Pulse generators for pulsed electric field exposure of biological cells and tissues

Cited by 71 publications

References 4 publications

Advances in Therapeutic Electroporation to Mitigate Muscle Contractions

Advances in Therapeutic Electroporation to Mitigate Muscle Contractions

High-Order Quadratures for the Solution of Scattering Problems in Two Dimensions

Blumlein Configuration for High-Repetition-Rate Pulse Generation of Variable Duration and Polarity Using Synchronized Switch Control

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