Pulse-Labeling Patterns of Microsomal Ribonucleic Acid Extracted with Lauryltrimethylammonium Chloride<sup>*</sup>

Kuff, Edward L.; Hymer, Wesley C.

doi:10.1021/bi00867a023

Cited by 27 publications

(2 citation statements)

References 28 publications

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“…The isotopicalIy labelled wheat-embryo RNA was prepared by imbibing wheat embryos, for 24 h, in a medium containing (")-labelled adenosine, guanosine, cytidine, and uridine (1 ' $1, and gel filtration of the NaCl-soluble fraction (2.5 M9 0") of wheat-embryo ( W I R N A was used to isolate electrophoreticalIy pure 5 S ("1-rRNA (I 8). Preparative sucrose density-gradient centrifugation ( 19,16 ) of the NaCI-insoluble fraction (2.5 M , 0 " ) of wheat-embryo WNA was used to prepare unlabelled 18 S and 26 S rRNA (11).…”

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confidence: 99%

A Possible Role for 5 S rRNA as a Bridge Between Ribosomal Subunits

Azad¹,

Lane²

1973

Can. J. Biochem.

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Toro!lt(p, Ontario M5S IA8 Received October 3, I973 M A D , A. A., and LANE, B. G. A possible role for 5 S rRNA as a bridge between ribosomal subunits. Can. J. Biochem. 51,1669-1 672 ( 1973 ).18 S rRNA is a high molecular weight polyribonucleotide found in the smnli subunit, and 26 S rRNA is a lllgh molecular weight polyribonucleotide found in the iarge subunit, whereas 5 S rRNA and 5.8 S rRNA are low molecular weight ("satellite") polyribonucleotides confined to the large subunit of wheat-embryo ribosomes. Under the same conditions in which 5.8 S rRNA is known to complex efficiently and preferentially with 26 S rRNA, it has been observed that 5 S rRNA complexes eficiently and preferentially with 18 S rRNA. Since 5 S rRNA is a component of the large ribosomal subunit, but it complexes preferentially with 18 S rRNA, which is a component of the small ribosomal subunit, it has been proposed that 5 S rRNA may serve as a "bridge" to mediate reversible association between the small and Barge ribosomal subunits. The possible role that a polycistronic precursor of rRNA might be visualized to play in the biogenesis and assembly of reversibly associating ribosomal subunits is alluded to in the present context. &AD, A. A., et LANE, B. G. A possible role for 5 S rRNA as a bridge between ribosomal subunits. Can. B. Biochem. 51,1669-1672 4 19773).Bans les ribosomes de l'embryon de bl6, le rRNA 18 S est un polyribornucltotide de poids mol6culaire e'levt contenu dans la petite sous-unit6 et le rRNA 26 S est un polyribonucl6otide de poids mol6culaire e'ievh corntenu dans la grande sous-unit6. Par contre, le rRNA 5 S et le rRNA 5.8 S sont des polyribonucltotides de fcrible poids moltculaire ("satellite") confinks B la grande sous-unit6. Bans les memes conditions oh le rRNA 5.8 S est reconnu pour former de f a~o n efficace des complexes de prkfirence avec le rRNA 26 S, on observe que le rRNA 5 S s'associe de prCf6rence et de faSon efficace avec le rRNA 18 S. Comme le rRNA 5 S est un constituant dle la grdande sous-unit6 ribosomique et qu'il s'associe de pi-ifhence avec le rRNA 18 S qui est un constituant de la petite sous-urnit6, on a propost que Ie rRNA 5 S pouvait &re le "pont" qui permettrait I'association rkversible entre la petite et la grande sous-unit6 ribosomique. Nous discutorns du r61e possible que pourrait joues un pr6canrseur polycistronique du RNA dans la biog6nbse et I'assernblage des sous-unitts ribosomiques s'associant de facon rtversible.[Traduit par le journal]

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confidence: 99%

A Possible Role for 5 S rRNA as a Bridge Between Ribosomal Subunits

Azad¹,

Lane²

1973

Can. J. Biochem.

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“…All sucrose solutions were pretreated with bentonite before use. RNA was analysed on gradients as described by KUFF and HYMER (1966) and polyribosornes were analysed as described previously (CAWAGNONI and MAHLER, 1967). RNA gradients were centrifuged at 4°C in the SW-25-1 rotor of the Spinco Model L-2 ultracentrifuge for 15 h at 25,000rev./min (63,50Og), or 20h at 23,000 rev./min (54,ooOg).…”

Section: Materials a N D Methodsmentioning

confidence: 99%

Fractionation of the Rna Components of Rat Brain Polysomes

Campagnoni¹,

Dutton²,

Mahler³

et al. 1971

Journal of Neurochemistry

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Abstract— The incorporation in vivo of [3H]uridine into the RNA isolated from the free polyribosomes of rat cerebral cortex was studied. Sedimentation in sucrose gradients showed that initially (at times less than 60 min after injection of precursor) the label was associated with a heterodisperse species, while at longer times there was an increased coincidence of label with stable rRNA. Further fractionation was accomplished by means of differential extraction with phenol and analysis on polyacrylamide‐agarose gels. Most of the rapidly labelled RNA was concentrated in a fraction obtained at pH 8‐3 and 40°C. The base composition of this fraction differed greatly from that of rRNA, preribosomal RNA and DNA. Analysis by electrophoresis on polyacrylamide‐agarose gels showed it to be composed of several distinct species in addition to residual 18 and 28S rRNA. Most of the latter was concentrated in a fraction extracted at pH 60 at 0°C.

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