Pulse length variation causing spectral distortions in OPO-based hyperspectral coherent Raman scattering microscopy

Pilger, Christian; Hachmeister, Henning; Greife, Paul; Weiss, A. H.; Wiebusch, Gerd; Huser, Thomas

doi:10.1364/oe.26.028312

Cited by 4 publications

(3 citation statements)

References 23 publications

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“…Another advantage is that the pulse‐width remains constant over the entire tuning range, so there is no need for additional pulse‐width control as in commercial OPO hyperspectral operation. [ 48 ] The low‐intensity fluctuations and the high power spectral density of both the pump and Stokes beams enable HS‐CRS microscopy, particularly SRS microscopy with excellent signal‐to‐noise ratio (SNR). We demonstrated that the SNR of the SRS images is at least similar to those obtained by a commercial OPO.…”

Section: Discussionmentioning

confidence: 99%

Hyperspectral Coherent Raman Scattering (CRS) Microscopy Based on a Rapidly Tunable and Environmentally Stable Fiber Laser

Kong,

Pilger,

Kunisch

et al. 2023

Laser & Photonics Reviews

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Coherent Raman scattering (CRS) microscopy is an attractive label‐free imaging method providing high chemical sensitivity, sub‐micron spatial resolution, and video‐rate imaging speed. When extended to hyperspectral CRS (HS‐CRS), it is capable of distinguishing molecules even in complex heterogeneous systems by probing multiple Raman resonances. Solid‐state lasers, associated with their high cost, large footprint, and slow tuning speed, currently limit the widespread application of HS‐CRS. Fiber lasers with comparable properties usually suffer from poor noise performance or instabilities during wavelength tuning. A tunable two‐color fiber laser is demonstrated that can be electronically tuned at rates of up to 1 kHz. The use of polarization‐maintaining fibers and an all‐optical synchronization mechanism guarantees its mode‐locking stability. The superior noise performance of this system is evaluated by the side‐by‐side comparison with data obtained by a commercial optical parametric oscillator (OPO)‐based laser. Large‐area multimodal imaging of liver tissue sections surgically resected from a patient suffering from a non‐neoplastic liver parenchyma with micronodular cirrhosis in combination with a multilocular hepatocellular carcinoma is demonstrated. By exploiting the fast‐hyperspectral scanning capability of the laser and multivariate statistical analysis, virtual staining of tissue sections and a comparison of the diagnostic properties of HS‐CRS to the classical histopathological approach are achieved.

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Section: Discussionmentioning

confidence: 99%

Hyperspectral Coherent Raman Scattering (CRS) Microscopy Based on a Rapidly Tunable and Environmentally Stable Fiber Laser

Kong,

Pilger,

Kunisch

et al. 2023

Laser & Photonics Reviews

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“…readily built into the popular scan control software ScanImage [19], early open access versions of which are still available and which we e.g. use to operate custom-build coherent Raman scattering microscopes [29]. For the acquisition of a single-raw image, the EOM first shutters the laser beam and the scanning mirrors are moved to the start of the first illuminated line of the overall pattern.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Super-resolution fluorescence microscopy by line-scanning with an unmodified two-photon microscope

Pilger

Pospíšil

Müller

et al. 2021

Phil. Trans. R. Soc. A.

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Fluorescence-based microscopy as one of the standard tools in biomedical research benefits more and more from super-resolution methods, which offer enhanced spatial resolution allowing insights into new biological processes. A typical drawback of using these methods is the need for new, complex optical set-ups. This becomes even more significant when using two-photon fluorescence excitation, which offers deep tissue imaging and excellent z-sectioning. We show that the generation of striped-illumination patterns in two-photon laser scanning microscopy can readily be exploited for achieving optical super-resolution and contrast enhancement using open-source image reconstruction software. The special appeal of this approach is that even in the case of a commercial two-photon laser scanning microscope no optomechanical modifications are required to achieve this modality. Modifying the scanning software with a custom-written macro to address the scanning mirrors in combination with rapid intensity switching by an electro-optic modulator is sufficient to accomplish the acquisition of two-photon striped-illumination patterns on an sCMOS camera. We demonstrate and analyse the resulting resolution improvement by applying different recently published image resolution evaluation procedures to the reconstructed filtered widefield and super-resolved images. This article is part of the Theo Murphy meeting issue ‘Super-resolution structured illumination microscopy (part 1)'.

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Tailoring the pulse characteristics of LiB₃O₅-based femtosecond optical parametric oscillators by temperature tuning

Qin,

Yu,

Yang

et al. 2024

Opt. Lett.

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We show that the phase mismatch in LiB3O5 (LBO) crystals is approximately proportional to the temperature deviation from the phase-matching temperature of crystals. Moreover, a fixed temperature deviation would result in approximately the same amount of phase mismatch over a large range of wavelengths. Based on this, we demonstrate that the pulse characteristics of LBO-based femtosecond optical parametric oscillators (OPOs) can be tailored by simply tuning the crystal temperature. Experimentally, at a temperature deviation of 10°C, nearly transform-limited optical pulses were obtained over a tuning range of 780–910 nm, and at a temperature deviation of −9°C, linearly chirped pulses with broadened spectra were obtained, which were de-chirped to be nearly transform-limited ones outside the cavity. The capability of generating femtosecond pulses with different pulse characteristics and at various wavelengths would benefit many applications, including spectroscopy and Raman microscopy.

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Pulse length variation causing spectral distortions in OPO-based hyperspectral coherent Raman scattering microscopy

Cited by 4 publications

References 23 publications

Hyperspectral Coherent Raman Scattering (CRS) Microscopy Based on a Rapidly Tunable and Environmentally Stable Fiber Laser

Hyperspectral Coherent Raman Scattering (CRS) Microscopy Based on a Rapidly Tunable and Environmentally Stable Fiber Laser

Super-resolution fluorescence microscopy by line-scanning with an unmodified two-photon microscope

Tailoring the pulse characteristics of LiB₃O₅-based femtosecond optical parametric oscillators by temperature tuning

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Pulse length variation causing spectral distortions in OPO-based hyperspectral coherent Raman scattering microscopy

Cited by 4 publications

References 23 publications

Hyperspectral Coherent Raman Scattering (CRS) Microscopy Based on a Rapidly Tunable and Environmentally Stable Fiber Laser

Hyperspectral Coherent Raman Scattering (CRS) Microscopy Based on a Rapidly Tunable and Environmentally Stable Fiber Laser

Super-resolution fluorescence microscopy by line-scanning with an unmodified two-photon microscope

Tailoring the pulse characteristics of LiB3O5-based femtosecond optical parametric oscillators by temperature tuning

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Product

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Tailoring the pulse characteristics of LiB₃O₅-based femtosecond optical parametric oscillators by temperature tuning