Pulse propagation by a capacitive mechanism drives embryonic blood flow

Anton, Halina; Harlepp, Sébastien; Ramspacher, Caroline; Wu, David; Monduc, Fabien; Bhat, Sandeep; Liebling, Michael; Paoletti, C; Charvin, Gilles; Freund, Jonathan B.; Vermot, Julien

doi:10.1242/dev.096768

Cited by 54 publications

(73 citation statements)

References 29 publications

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“…Another intriguing possibility is that particular patterns of blood pressure could stretch endocardial cells in a manner that provokes specific cellular responses. In future work, a combination of techniques that directly manipulate flow direction or induce stretch (Anton et al, 2013;Marjoram et al, 2016;Sidhwani & Yelon, 2019), together with controlled flow environments to house explanted hearts (Cao & Poss, 2016;Wong et al, 2016), could enable the dissection of potential influences of specific functional cues on OFT morphogenesis.…”

Section: Discussionmentioning

confidence: 99%

Cardiac function modulates endocardial cell dynamics to shape the cardiac outflow tract

Sidhwani¹,

Boezio

Yang³

et al. 2019

Preprint

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Physical forces are important participants in the cellular dynamics that shape developing organs. During heart formation, for example, contractility and blood flow generate biomechanical cues that influence patterns of cell behavior. Here, we address the interplay between function and form during the assembly of the cardiac outflow tract (OFT), a crucial connection between the heart and vasculature that develops while circulation is underway. In zebrafish, we find that the OFT expands via accrual of both endocardial and myocardial cells. However, when cardiac function is disrupted, OFT endocardial growth ceases, accompanied by reduced proliferation and reduced addition of cells from adjacent vessels. The TGFβ receptor Acvrl1 is required for addition of endocardial cells, but not for their proliferation, indicating distinct regulation of these essential cell behaviors. Together, our results suggest that cardiac function modulates OFT morphogenesis by triggering endocardial cell accumulation that induces OFT lumen expansion and shapes OFT dimensions.

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Section: Discussionmentioning

confidence: 99%

Cardiac function modulates endocardial cell dynamics to shape the cardiac outflow tract

Sidhwani¹,

Boezio

Yang³

et al. 2019

Preprint

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“…This optical technique has originally been used for in vitro measurement of viscoelastic properties of single molecules (Klajner et al, 2010;Smith et al, 1996) or single cells Dao et al, 2003). Recently, this technique has been applied in vivo for quantifying cell junction elasticity (Bambardekar et al, 2015;Sugimura et al, 2016) and proepicardial cell adhesion (Peralta et al, 2013), as well as for trapping circulating blood cells, thereby addressing haemodynamic forces in zebrafish (Anton et al, 2013) and mouse (Zhong et al, 2013). In atomic force microscopy (AFM), a cantilever is transiently brought in contact with the cell surface (Tartibi et al, 2015), cell nuclei (Lanzicher et al, 2015), or biological material such as cell-derived matrices (Tello et al, 2016).…”

Section: Optical Tweezers In Vivomentioning

confidence: 99%

Seeing is believing: multi-scale spatio-temporal imaging towards in vivo cell biology

Follain

Mercier

Osmani

et al. 2016

Journal of Cell Science

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Life is driven by a set of biological events that are naturally dynamic and tightly orchestrated from the single molecule to entire organisms. Although biochemistry and molecular biology have been essential in deciphering signaling at a cellular and organismal level, biological imaging has been instrumental for unraveling life processes across multiple scales. Imaging methods have considerably improved over the past decades and now allow to grasp the inner workings of proteins, organelles, cells, organs and whole organisms. Not only do they allow us to visualize these events in their most-relevant context but also to accurately quantify underlying biomechanical features and, so, provide essential information for their understanding. In this Commentary, we review a palette of imaging (and biophysical) methods that are available to the scientific community for elucidating a wide array of biological events. We cover the most-recent developments in intravital imaging, light-sheet microscopy, superresolution imaging, and correlative light and electron microscopy. In addition, we illustrate how these technologies have led to important insights in cell biology, from the molecular to the whole-organism resolution. Altogether, this review offers a snapshot of the current and state-of-the-art imaging methods that will contribute to the understanding of life and disease.

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“…In the developing cardiovascular system, biomechanics is key for modulating flow propagation (Anton et al, 2013). In the teleost heart, the OFT constitutes a specialized organ comprising the bulbus arteriosus (BA).…”

Section: Introductionmentioning

confidence: 99%

Mechanically activated Piezo channels control outflow tract valve development through Yap1 and Klf2-Notch signaling axis

Duchemin

Vignes

Vermot

2019

Preprint

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Mechanical forces are well known for modulating heart valve developmental programs. Yet, it is still unclear how genetic programs and mechanosensation interact during heart valve development. Here, we assessed the mechanosensitive pathways involved during zebrafish outflow tract (OFT) valve development in vivo. Our results show that the hippo effector Yap1, Klf2, and the Notch signaling pathway are all essential for OFT valve morphogenesis in response to mechanical forces, albeit active in different cell layers. Furthermore, we show that Piezo and TRP mechanosensitive channels are essential for regulating these pathways. In addition, live reporters reveal that piezo controls Klf2 and Notch activity in the endothelium and Yap1 expression in the smooth muscle progenitors to coordinate OFT valve morphogenesis. Together, this work identifies a unique morphogenetic program during OFT valve formation and places Piezo as a central modulator of the cell response to forces in this process.

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Pulse propagation by a capacitive mechanism drives embryonic blood flow

Cited by 54 publications

References 29 publications

Cardiac function modulates endocardial cell dynamics to shape the cardiac outflow tract

Cardiac function modulates endocardial cell dynamics to shape the cardiac outflow tract

Seeing is believing: multi-scale spatio-temporal imaging towards in vivo cell biology

Mechanically activated Piezo channels control outflow tract valve development through Yap1 and Klf2-Notch signaling axis

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