Pulse requirements for electron diffraction imaging of single biological molecules

Hau-Riege, S; London, R; Chapman, H

doi:10.2172/15014820

Cited by 2 publications

(1 citation statement)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The highlights of our research include: the development, improvement, and testing of a hydrodynamic model of the Coulomb explosion of particles (Hau-Riege, 2004b); the development of a model that estimates the required fluence to classify and average diffraction patterns ; the integration of those models to determine resolution limits (Hau-Riege, 2005); the development of models for X-ray-induced damage in solids; the introduction of a new algorithm for automatic reconstruction of an image from its diffraction pattern without the need for any additional low-resolution information ; successful X-ray diffraction imaging experiments carried out at the Advanced Light Source at LBNL (the world's first true lensless coherent diffraction image formed without a priori information, and the world's first full 3D coherent diffraction image) …”

Section: Research and Resultsmentioning

confidence: 99%

FY05 LDRD Final Report, A Revolution in Biological Imaging

Chapman¹,

Bajt²,

Balhorn³

et al. 2006

Self Cite

View full text Add to dashboard Cite

X-ray free-electron lasers (XFELs) are currently under development and will provide a peak brightness more than 10 orders of magnitude higher than modern synchrotrons. The goal of this project was to perform the fundamental research to evaluate the possibility of harnessing these unique x-ray sources to image single biological particles and molecules at atomic resolution. Using a combination of computational modelling and experimental verification where possible, we showed that it should indeed be possible to record coherent scattering patterns from single molecules with pulses that are shorter than the timescales for the degradation of the structure due to the interaction with those pulses. We used these models to determine the effectiveness of strategies to allow imaging using longer XFEL pulses and to design validation experiments to be carried out at interim ultrafast sources. We also developed and demonstrated methods to recover threedimensional (3D) images from coherent diffraction patterns, similar to those expected from XFELs. Our images of micron-sized test objects are the highest-resolution 3D images of any noncrystalline material ever formed with x rays. The project resulted in 14 publications in peer-reviewed journals and four records of invention.

show abstract

Section: Research and Resultsmentioning

confidence: 99%