Pulsed electric field-assisted extraction of carotenoids from fresh biomass of  Rhodotorula glutinis

Martínez, Juan Manuel; Delso, Carlota; Angulo, Javier; Álvarez, Ignacio; Raso, Javier

doi:10.1016/j.ifset.2018.04.012

Cited by 54 publications

(23 citation statements)

References 30 publications

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“…A small proportion of inactivated X. dendrorhous cells were impermeable to PI after application of mild intensity PEF treatments that caused low inactivation. This observation, previously reported by other authors, suggests that in these cells, the pore resealing is a physical process rather than a process with biosynthetic requirements (Martínez et al, 2018). Therefore, quantification of the proportion of inactivated cells is a suitable index for estimation of the number of irreversible electroporation cells when the inactivation is higher of a given value, that in the case of the strains of X. dendrorhous investigated was around 70%.…”

Section: Discussionsupporting

confidence: 55%

“…On the other hand, a higher percentage of inactivated cells compared to PI permeabilized cells were observed when the inactivation was below this value. The lack of correlation between the percentage of dead and permeabilized cells when the inactivation effect was low could indicate that a certain number of inactivated cells were able to recover the integrity of their membrane even after losing their viability (García et al, 2007;Luengo et al, 2014;Martínez et al, 2018). Therefore, this proportion of inactivated cells would behave just like those non-electroporated cells during the extraction of intracellular compounds.…”

Section: Sensitivity Of X Dendrorhous Cect 11028 and X Dendrorhous mentioning

confidence: 99%

“…Over the last years, several cell wall disruption processes such as chemical (acid extraction), enzymatic and physical or mechanical (ultrasound, microwave, high-pressure homogenization) methods have been evaluated to facilitate the recovery of intracellular carotenoids from X. dendrorhous (Choi et al, 2007;Ni et al, 2008;Michelon et al, 2012;Gogate and Nadar, 2015;Hasan et al, 2016;Urnau et al, 2018;Zhuang et al, 2020). However, those techniques present some disadvantages such as high implementation costs, long extraction time, excessive cell destruction and high cost of purification of the target molecule (Martínez et al, 2018). Furthermore, most carotenoids extraction techniques require the drying of biomass with subsequent extraction with a large amount of organic solvent.…”

Section: Introductionmentioning

confidence: 99%

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Enzymatic Processes Triggered by PEF for Astaxanthin Extraction From Xanthophyllomyces dendrorhous

Aguilar-Machado

Delso

Martínez

et al. 2020

Front. Bioeng. Biotechnol.

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The aim of this study was to evaluate the potential of pulsed electric fields (PEF) to improve the extraction of the lipid-soluble astaxanthin from fresh biomass of a wild-type (CECT 11028) and mutant (ATCC 74219) Xanthophyllomyces dendrorhous strain using ethanol as solvent. Inactivation and propidium uptake studies revealed that inactivation is a good index for estimated the proportion of irreversible permeabilized cells when inactivation is higher than 70% in the two strains. Ethanol was ineffective for extracting carotenoids from the PEF-treated cells (20 kV/cm, 135 µs) of the two strains. However, after aqueous incubation of PEF-treated X. dendrorhous ATCC 74219 cells for 12 h, up to 2.4 ± 0.05 mg/g dried weight (d.w.) of carotenoids were extracted in ethanol. From total carotenoid extracted, around 84% corresponded to all-trans astaxanthin. The detection and quantification of esterase activity in the supernatant and the relationship between the percentage of esterase activity quantified and the amount of carotenoids extracted indicate that the extraction of astaxanthin was mediated by enzymatic esterase activity triggered by PEF during incubation. On the other hand, the formation of a large lipid globule into the cytoplasm of PEF-treated X. dendrorhous CECT 11028 cells during aqueous incubation prevented carotenoid extraction. The process developed in this investigation represents a more sustainable and greener method that those previously used for extracting astaxanthin from yeast.

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Section: Discussionsupporting

confidence: 55%

Section: Sensitivity Of X Dendrorhous Cect 11028 and X Dendrorhous mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enzymatic Processes Triggered by PEF for Astaxanthin Extraction From Xanthophyllomyces dendrorhous

Aguilar-Machado

Delso

Martínez

et al. 2020

Front. Bioeng. Biotechnol.

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“…Flow cytometry measurements detected morphological changes in PEF‐treated R. glutinis cells during aqueous incubation caused by the autolysis triggering effect of electroporation. It was postulated that this improvement in the release of carotenoids was produced through the lysis of the bonds of carotenoids with other components of the cytoplasm by enzymes liberated from the organelles, as a result of the distressing osmotic conditions of the cytoplasm after electroporation (Martínez, Delso, Angulo, Álvarez, & Raso, ).…”

Section: Pulsed Electric Fields Technology For Enhancing Extraction Omentioning

confidence: 99%

Pulsed electric field‐assisted extraction of valuable compounds from microorganisms

Martínez

Delso

Álvarez

et al. 2020

Comp Rev Food Sci Food Safe

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137

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Microorganisms (bacteria, yeast, and microalgae) are a promising resource for products of high value such as nutrients, pigments, and enzymes. The majority of these compounds of interest remain inside the cell, thus making it necessary to extract and purify them before use. This review presents the challenges and opportunities in the production of these compounds, the microbial structure and the location of target compounds in the cells, the different procedures proposed for improving extraction of these compounds, and pulsed electric field (PEF)‐assisted extraction as alternative to these procedures. PEF is a nonthermal technology that produces a precise action on the cytoplasmic membrane improving the selective release of intracellular compounds while avoiding undesirable consequences of heating on the characteristics and purity of the extracts. PEF pretreatment with low energetic requirements allows for high extraction yields. However, PEF parameters should be tailored to each microbial cell, according to their structure, size, and other factors affecting efficiency. Furthermore, the recent discovery of the triggering effect of enzymatic activity during cell incubation after electroporation opens up the possibility of new implementations of PEF for the recovery of compounds that are bounded or assembled in structures. Similarly, PEF parameters and suspension storage conditions need to be optimized to reach the desired effect. PEF can be applied in continuous flow and is adaptable to industrial equipment, making it feasible for scale‐up to large processing capacities.

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“…The intake of chemically synthesized carotenoids is considered less safe than that of naturally occurring carotenoids. Therefore, natural carotenoids have the advantage of higher customer preference and are gaining more attention (Martínez, Delso, Angulo, Álvarez, & Raso, ; Rodriguez‐Amaya, ).…”

Section: Introductionmentioning

confidence: 99%

Metabolomic Profiling of Carotenoid Constituents in Physalis peruviana During Different Growth Stages by LC‐MS/MS Technology

Chen

Zheng

2019

Journal of Food Science

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With the current ongoing changes in global food demands, natural carotenoids are preferred by consumers and are gaining attention among food scientists and producers alike. Metabolomic profiling of carotenoid constituents in Physalis peruviana during distinct on-tree growth stages was performed with liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology. The results show that the β rings of β-carotene are hydroxylated with great efficiency, and there is a continual synthesis of zeaxanthin at half-ripe and full-ripe stages, which is confirmed by relating the zeaxanthin content to that of its precursor (β-carotene). Lutein was, in terms of mass intensity, the most abundant carotenoid constituent (64.61 µg/g at the half-ripe stage) observed in this study. In addition, γ -carotene, which is rare in dietary fruits and vegetables, was detected in the mature and breaker stages, albeit at a relatively low level. The results suggest that when we consider the variation in carotenoid content during different growth stages, Physalis peruviana can be considered a good source of natural carotenoids.

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Pulsed electric field-assisted extraction of carotenoids from fresh biomass of Rhodotorula glutinis

Cited by 54 publications

References 30 publications

Enzymatic Processes Triggered by PEF for Astaxanthin Extraction From Xanthophyllomyces dendrorhous

Enzymatic Processes Triggered by PEF for Astaxanthin Extraction From Xanthophyllomyces dendrorhous

Pulsed electric field‐assisted extraction of valuable compounds from microorganisms

Metabolomic Profiling of Carotenoid Constituents in Physalis peruviana During Different Growth Stages by LC‐MS/MS Technology

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