Pulsed electric field reduces the permeability of potato cell wall

Abstract

The effect of the application of pulsed electric fields to potato tissue on the diffusion of the fluorescent dye FM1-43 through the cell wall was studied. Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with one 1 ms rectangular pulse, before application of FM1-43 and microscopic examination. Our results show a slower diffusion of FM1-43 in the electropulsed tissue when compared with that in the non-pulsed tissue, suggesting that the electric field decreased the cell wall permeability. This is a fast response that is already detected within 30 s after the delivery of the electric field. This response was mimicked by exogenous H2O2 and blocked by sodium azide, an inhibitor of the production of H2O2 by peroxidases.

Effects of nanosecond pulsed electric field exposure on arabidopsis thaliana

“…Galindo et al observed a decrease in cell wall permeability of potato cells resulting from PEF treatment. They found evidence, that production of H 2 O 2 by cell wall associated peroxidases may be involved in that response [42]. H 2 O 2 is known to play a role in plant responses, involved in wound healing [43].…”

Effect of pulsed electrical fields on the structural properties that affect french fry texture during processing

“…It is well known that conductivity in the membrane increases immediately after the pulsed treatment (Angersbach, Heinz, & Knorr, 2000) and the membrane becomes more permeable as the conductivity increases (Pereira, Galindo, Vicente, & Dejmek, 2009;Gómez Galindo, Vernier, Dejmek, Vicente, & Gundersen, 2008;Bazhal, Lebovka, & Vorobiev, 2003). This increase in membrane permeability seems to re-establish the equilibrium between the electrochemical and electrical potential differences between the cell plasma and the extracellular surroundings (Raso-Pueyo & Heinz, 2010).…”

“…By increasing field strength, pulse duration and number of pulses, the density (number and size) of pores in the cell membrane and in the cell wall increases, and can cause rupture of the membrane (Asavasanti, Ristenpart, Stroeve, & Barrett, 2011;Pereira, Galindo, Vicente, & Dejmek, 2009;Gómez Galindo, Vernier, Dejmek, Vicente, & Gundersen, 2008;Arevalo, Ngadi, Bazhal, & Raghavan, 2004;Bazhal, Lebovka, & Vorobiev, 2003;Fincan & Dejmek, 2002;Knorr & Angersbach, 1998;Zimmermann, 1986). The cell may also be damaged by the overheating of the membrane surface, or chemical imbalances caused by transport out of the cell.…”

“…As the intensity of the field strength increased, the number of dead cells (not stained) increased, with the inner medulla and pith tissue being the most affected. The response of a cell membrane that was associated with induced permeabilization has also been studied (Gómez Galindo, Vernier, Dejmek, Vicente, & Gundersen, 2008). They M A N U S C R I P T…”

Effects of Pulsed Electric Field on the Viscoelastic Properties of Potato Tissue

“…This investigation as well as others in which changes on rheological properties of PEFtreated potatoes are addressed, 9,13,14 would deal with a tissue in which cell membranes permeability properties have been damaged with a consequent leakage of cell components. However, based on previous publications of our group, 15,16 it is expected that when mild electric field treatments are used and the cell membranes have the capacity to recover their functional properties, metabolic responses may create, in a time scale of seconds, a very dynamic cellular system in terms of variations of cell turgor as well as structural changes. Therefore, dynamic rheological measurements during the PEF treatment would provide valuable information on the behavior of tissues under PEF stress.…”