Pulsed electrochemical detection of orotic acid by an activated potential waveform at a gold working electrode following anion-exchange chromatography

Cataldi, Tommaso R. I.; Nardiello, Donatella; Ciriello, Rosanna; Guerrieri, Antonio

doi:10.1016/j.chroma.2005.12.049

Cited by 7 publications

(3 citation statements)

References 43 publications

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“…OA is abundant in serum and urine of patients with hereditary orotic aciduria or enzyme defects in the urea cycle. The development and optimization of sensitive techniques to measure orotate concentration in biological samples, without other compounds interference, was prompted by studies on dairy products, as well as the discovery of OA in human fluids caused by inborn errors of pyrimidine metabolism [22][23][24][25][26][27][28]. In studies on the development of HPLC methodology, earlier microbiological, enzymatic, polarographic and colorimetric experiments were carefully considered [29][30][31].…”

Section: Discussionmentioning

confidence: 99%

The Strange Case of Orotic Acid: The Different Expression of Pyrimidines Biosynthesis in Healthy Males and Females

Chiara,

Allegra,

Mula

et al. 2023

JPM

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Orotic acid (OA) is an intermediate metabolite of pyrimidine nucleotide biosynthesis and represents a minor diet constituent. The measurement of urinary orotic acid is useful in confirming the diagnosis of hereditary metabolic diseases. Moreover, it could be of interest to know how the physiological concentration of this metabolite changes in relation to different conditions of clinical normality. The purpose of this study was to determine the orotic acid concentration in the urine of healthy patients, to observe normal oroticuria and to evaluate if the expression of pyrimidine intermediate biosynthesis differs between healthy males and females. The orotic acid concentration in urine was performed via the ICH M10-validated analytical method. Unexpectedly, females showed a greater oroticuria than males in pediatric age (0–10); conversely, we did not find significant differences until 70 years of age. The LC-MS/MS method was suitable for use in the differential diagnosis of hereditary metabolic disease and metabolic monitoring of anticancer drug-induced toxicity. The analytical protocol was found to be rapid and ideal, and was used in the routine analysis of a clinical chemistry laboratory. The biochemical aspects related to the expression of pyrimidine biosynthesis should be further investigated in light of the obtained results.

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Section: Discussionmentioning

confidence: 99%

The Strange Case of Orotic Acid: The Different Expression of Pyrimidines Biosynthesis in Healthy Males and Females

Chiara,

Allegra,

Mula

et al. 2023

JPM

View full text Add to dashboard Cite

show abstract

“…The molecular structure of orotic acid is given in Figure 1. Recent experimental studies have demonstrated that it is involved in carcinogenesis, hepatic lipid storage and renal toxicity [14]. Several studies have shown that repeated dosing of orotic acid promotes the formation of tumors initiated by various known carcinogenic substances [15].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Different Anions in Milk Samples Using Ion Chromatography with Conductivity Detection

Destanoğlu

Yılmaz

2016

Journal of the Turkish Chemical Society, Section A: Chemistry

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Abstract:In this study, a simple method for simultaneous determination of chloride, nitrate, sulfate, iodide, phosphate, thiocyanate, perchlorate, and orotic acid in milk samples was investigated. The method involves the use of dialysis cassettes for matrix elimination, followed by ion chromatographic analysis of the anions on a high capacity anion exchange column with suppressed conductivity detection. The novelty of the proposed method was the use of dialysis process which did not need any chemical and organic solvent for elimination of macromolecules such as fat, carbohydrates and proteins from milk samples. External standard calibration curves for these analytes were linear with good correlation coefficients. The relative standard deviations of analyte concentrations were acceptable both inter-day and intra-day evaluations. Under optimized conditions, the limit of detection (Signal-to-Noise ratio = 3) for chloride, phosphate, thiocyanate, perchlorate, iodide, nitrate, sulfate, and orotate was found to be 0. 012, 0.112, 0.140, 0.280, 0.312, 0.516, 0.520, and 0.840 mg L −1 , respectively. Significant results were obtained for various spiked milk samples with % recovery in the range of 93.88 -109.75 %. The proposed method was successfully applied to milk samples collected from Istanbul markets. The advantages of the method described herein are reagent-free, simple, and reliable.

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Recent developments in electrochemical flow detections—A review

Trojanowicz

2011

Analytica Chimica Acta

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Pulsed electrochemical detection of orotic acid by an activated potential waveform at a gold working electrode following anion-exchange chromatography

Cited by 7 publications

References 43 publications

The Strange Case of Orotic Acid: The Different Expression of Pyrimidines Biosynthesis in Healthy Males and Females

The Strange Case of Orotic Acid: The Different Expression of Pyrimidines Biosynthesis in Healthy Males and Females

Simultaneous Determination of Different Anions in Milk Samples Using Ion Chromatography with Conductivity Detection

Recent developments in electrochemical flow detections—A review

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