Pulsed Electromagnetic Field Stimulation Promotes Anti-cell Proliferative Activity in Doxorubicin-treated Mouse Osteosarcoma Cells

Muramatsu, Yoshitaka; Matsui, Toshimitsu; Deie, Masataka; Sato, Keiji

doi:10.21873/invivo.11026

Cited by 11 publications

(9 citation statements)

References 30 publications

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“…TAK1, a common upstream regulator of NF-κB, prevents apoptosis via NF-κB-induced downregulation of IκBα (26,27). This mechanism may partially explain why LIPUS treatment significantly reduced the levels of p-TAK1 (P<0.0001) and enhanced the levels of IκBα (P=0.0001) as a result of the induction of apoptosis in LM8 cells.…”

Section: Discussionmentioning

confidence: 96%

Inhibitory effects of low-intensity pulsed ultrasound sonication on the proliferation of osteosarcoma cells

et al. 2017

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Abstract. To date, there is limited data on the biological effects of low-intensity pulsed ultrasound (LIPUS) on primary malignant bone tumors. The purpose of the present study was to investigate the antitumor effects of LIPUS on osteosarcoma cells. The effects of LIPUS on cell viability, induction of apoptosis, mitochondrial membrane potential and intracellular signaling molecules in the LM8 osteosarcoma cell line were investigated. LIPUS inhibited cell viability (P=0.0022) and mitochondrial membrane potential (P=0.0019) in LM8 cells. Flow cytometry analysis and terminal deoxynucleotidyl transferase dUTP nick end labeling staining revealed significantly higher numbers of apoptotic (P<0.0001) and necrotic cells (P=0.0091) compared with cells without treatment. LIPUS treatment significantly increased phosphorylated Akt (P<0.0001) and IκBα (P=0.0001) levels, and reduced phosphorylated mitogen-activated protein kinase 7 (P<0.0001) and phosphorylated checkpoint kinase 1 (P=0.0008) levels. These results suggest that LIPUS is a non-invasive adjuvant therapy that is able to inhibit cellular proliferation in osteosarcoma cells.

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Section: Discussionmentioning

confidence: 96%

Inhibitory effects of low-intensity pulsed ultrasound sonication on the proliferation of osteosarcoma cells

et al. 2017

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“…PEMF has been successfully applied to various diseases in several basic research experiments, most commonly including senile osteoporosis, osteoarthritis and cancer bone metastasis. However, few studies have reported the effects of PEMF on osteoclast differentiation and formation, and the associated signaling pathway mechanisms (31,32). A previous study demonstrated that PEMF exposure markedly inhibited RANKL-induced osteoclast formation from RAW264.7 macrophages, and that this effect resulted from suppression of intracellular Akt and mTOR protein phosphorylation.…”

Section: Discussionmentioning

confidence: 99%

Pulsed electromagnetic fields inhibit osteoclast differentiation in RAW264.7 macrophages via suppression of the protein kinase B/mammalian target of rapamycin signaling pathway

Lei

et al. 2018

Mol Med Report

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When bone resorption, aided by the activity of osteoclasts, exceeds bone formation induced by osteoblasts, bone metabolism loses equilibration, which results in the development of bone diseases, including osteoporosis. Pulsed electromagnetic fields (PEMFs) are known to be involved in various biological processes, including cell proliferation, differentiation and apoptosis. However, the exact mechanism of action of osteoclasts remains poorly understood. In the present study, the effects of PEMFs on osteoclast differentiation and associated signaling pathways were systematically investigated in RAW264.7 macrophages. RAW264.7 cells were induced by receptor activator of nuclear factor‑κB ligand (RANKL) to obtain osteoclasts in vitro. The results of the present study demonstrated that PEMF exposure decreased osteoclast formation, limited tartrate‑resistant acid phosphatase activity, contracted bone resorption area and inhibited osteoclastic specific gene and protein expression. Furthermore, western blot analysis indicated that PEMFs distinctly abolished the upregulation of phosphorylated‑protein kinase B (Akt), ‑mammalian target of rapamycin (mTOR) and ‑ribosome S6 protein kinase (p70S6K) induced by RANKL, which was consistent with the effects of pharmacological inhibitor perifosine and rapamycin. Therefore, the present study suggested that PEMFs reduced osteoclast formation from RAW264.7 macrophages via inhibition of the Akt/mTOR signaling pathway. These findings provided novel insight into the mechanisms through which PEMFs suppress osteoclast differentiation.

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“…NALM-6 cells were seeded at 1 9 10 5 cells/well in 96-well dishes and subcultured for 24 h, according to our previous report. [21] The cells were then cultured for 24, 48 and 72 h with 15 lM acrofolione A (1) or B (2), and they were then fixed in 70% ethanol and treated with 0.5% ribonuclease for 30 min. DNA was stained with 4 0 ,6-diamidino-2-phenylindole (DAPI, 1.2 lM) for 30 min at room temperature in the dark.…”

Section: Cell Cycle Analysismentioning

confidence: 99%

Acrofolione A and B, acetophenone dimers from Acronychia pendunculata, induce an apoptotic effect on human NALM-6 pre-B cell leukaemia cells

Matsui

Ito

Kato

et al. 2019

Journal of Pharmacy and Pharmacology

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Objectives We investigated the apoptotic activities of acrofolione A (1) and B (2) isolated from Acronychia pedunculata against a human pre‐B cell leukaemia cell line (NALM‐6) to explore the apoptosis‐related signalling molecules targeted by 1 and 2. Methods The apoptosis effects of 1 and 2 in NALM‐6 cells were investigated by TUNEL staining, annexin V, mitochondria membrane potential and caspase 3/7 activity. We carried out a protein array to explore the signalling molecules involved in apoptosis comprehensively. Key findings Acrofolione A (1) suppressed the growth of NALM‐6, K562 and HPB‐ALL cells (IC50 16.7 ± 1.9, 17.9 ± 0.3 and 10.1 ± 0.2 μm, respectively) more effectively than acrofolione B (2). Both compounds time‐dependently increased the number of NALM‐6 cells with abnormal nuclei, and increased the number of annexin V‐positive cells and decreased the mitochondrial membrane potential of NALM‐6 cells. Acrofolione A (1) markedly elevated caspase 3/7 activity and increased the number of TUNEL‐positive cells. Cells treated with either compound showed enhanced expression of cleaved PARP and cleaved caspase 3 and 7, and reduced survivin protein levels. Conclusions Acrofolione A (1) and B (2) may be useful in the treatment of various types of leukaemia.

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Pulsed Electromagnetic Field Stimulation Promotes Anti-cell Proliferative Activity in Doxorubicin-treated Mouse Osteosarcoma Cells

Cited by 11 publications

References 30 publications

Inhibitory effects of low-intensity pulsed ultrasound sonication on the proliferation of osteosarcoma cells

Inhibitory effects of low-intensity pulsed ultrasound sonication on the proliferation of osteosarcoma cells

Pulsed electromagnetic fields inhibit osteoclast differentiation in RAW264.7 macrophages via suppression of the protein kinase B/mammalian target of rapamycin signaling pathway

Acrofolione A and B, acetophenone dimers from Acronychia pendunculata, induce an apoptotic effect on human NALM-6 pre-B cell leukaemia cells

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