Pulsed-field gel electrophoresis for epidemiologic studies of Campylobacter hyointestinalis isolates

Salama, Sameeh M.; Tabor, Helen; Richter, Magdalena S.; Taylor, Diane E.

doi:10.1128/jcm.30.8.1982-1984.1992

Cited by 41 publications

(14 citation statements)

References 19 publications

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“…Pulsed-field gel electrophoresis has been used to compare chromosome restriction patterns in Acinetobacter calcoaceticus, Pseudomonas aeruginosa (1), Pseudomonas cepacia (2), Legionella pneumophila (25), Enterococcus faecalis (20), Mycoplasma hominis (13), Leptospira sp. (9), Campylobacter hyointestinalis (24), and Staphylococcus aureus (23,28). The technique is relatively simple, is applicable to a wide range of bacteria with little modification, and appears to have sufficient discriminatory power to be useful for epidemiological tracking.…”

Section: Discussionmentioning

confidence: 99%

DNA fingerprinting of Streptococcus pneumoniae strains by pulsed-field gel electrophoresis

Lefèvre¹,

Faucon²,

Sicard³

et al. 1993

J Clin Microbiol

146

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Pulsed-field gel electrophoresis of genomic DNA was carried out on Streptococcus pneumoniae strains to determine its value in the epidemiological survey of pneumococcal infections. Twenty-one clinical strains were chosen to cover a broad range of diversity according to geographic location, penicillin susceptibility, serotype, and multilocus enzyme electrophoresis (MLEE) pattern. The restriction endonucleases ApaI and SmaI were used to digest intact chromosomes, and the fragments were resolved by field inversion gel electrophoresis (FIGE). Each digest produced 10 to 19 fragments for comparison between strains. All the strains, including strains of the same serotype and strains with the same MLEE profile, had different FIGE patterns. In some cases, the restriction patterns differed by only a few fragment bands, and two isolates differed only in the location of a single DNA fragment. The polymorphism obtained with FIGE was greater than those obtained with serotyping and MLEE analysis. The stability of the FIGE profiles was established by testing of two independent clones derived from pneumococcus strain R36A. These results indicated that pulsed-field gel electrophoresis should be an effective tool for the typing of S. pneumoniae strains, capable of subdividing serotypes or MLEE types and of tracing the origin of pneumococcal strains.

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Section: Discussionmentioning

confidence: 99%

DNA fingerprinting of Streptococcus pneumoniae strains by pulsed-field gel electrophoresis

Lefèvre¹,

Faucon²,

Sicard³

et al. 1993

J Clin Microbiol

146

View full text Add to dashboard Cite

show abstract

“…Furthermore, Campylobacter fetus, Campylobacter hyointestinalis subsp. hyointestinalis, Campylobacter lari and Campylobacter upsaliensis have also been recognized as human pathogens, mainly causing gastroenteritis and/or septicaemia (Broczyk et al, 1987;Salama et al, 1992;Goossens et al, 1995;Moore et al, 2005;On, 2005). These four taxa would be underestimated because the isolation conditions for C. jejuni and C. coli (incubation at 42 u C on selective-ingredient-containing media) are inhibitory to certain strains of these four Campylobacter (Logan et al, 2001;Moore et al, 2005;On, 2005).…”

Section: Introductionmentioning

confidence: 99%

Development of a multiplex PCR assay for identification of Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalis subsp. hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis

Yamazaki

Taguchi

Seto

et al. 2007

Journal of Medical Microbiology

126

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A multiplex PCR assay has been developed for the identification of the six common Campylobacter taxa associated with human gastroenteritis and/or septicaemia, namely Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalis subsp. hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis. The assay was developed using a combination of newly designed and published primers. It provided a specific PCR product for each of the five Campylobacter species and the one subspecies, and each of the PCR products was sufficiently distinguished by a difference in size by agarose gel electrophoresis. On evaluation of efficacy with 142 Campylobacter strains, the assay correctly identified all strains as 1 of the 6 Campylobacter taxa. This multiplex PCR assay is a rapid, simple and practical tool for identification of the six Campylobacter taxa commonly associated with gastroenteritis and/or septicaemia in humans, and offers an effective alternative to conventional biochemical-based assays.

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“…Many different investigators have used PFGE successfully to subtype L. pneumophila (15,24,25), as well as Staphylococcus spp. (14), Klebsiella pneumoniae (21), Campylobacter hyointestinalis (22), and Enterococcus faecalis (9). We applied AP-PCR and PFGE to isolates collected from patients and environmental sites during investigations of seven unrelated outbreaks of Legionnaires' disease for which corresponding patient and environmental isolates as well as MAb subtyping information were available (3,4,13,16,27,29).…”

mentioning

confidence: 99%

Comparison of Legionella pneumophila isolates by arbitrarily primed PCR and pulsed-field gel electrophoresis: analysis from seven epidemic investigations

et al. 1995

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Arbitrarily primed PCR (AP-PCR) and pulsed-field gel electrophoresis (PFGE) subtyping were applied to clinical and environmental isolates from seven unrelated outbreaks of Legionnaires' disease. The patterns observed with each method matched patient isolates and the epidemiologically linked source of disease for each of the seven outbreaks. PFGE allowed more discrimination among various isolates, although AP-PCR usually gave comparable results. With both methods, certain patterns appeared to predominate in the comparison of the seven outbreaks. Of five clinical isolates not associated with the outbreaks, three gave profiles distinct from those observed in the outbreaks by both methods. This suggests that there are at least two predominant subtypes of Legionella pneumophila serogroup 1 associated with outbreaks. Investigations of outbreaks of legionellosis should employ either PFGE or AP-PCR in addition to monoclonal antibody analysis.

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Pulsed-field gel electrophoresis for epidemiologic studies of Campylobacter hyointestinalis isolates

Cited by 41 publications

References 19 publications

DNA fingerprinting of Streptococcus pneumoniae strains by pulsed-field gel electrophoresis

DNA fingerprinting of Streptococcus pneumoniae strains by pulsed-field gel electrophoresis

Development of a multiplex PCR assay for identification of Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalis subsp. hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis

Comparison of Legionella pneumophila isolates by arbitrarily primed PCR and pulsed-field gel electrophoresis: analysis from seven epidemic investigations

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